A freeze-fracture study on the developing satellite cells of spinal ganglia in the chick embryo

Carvalho, Claudio A. Ferraz de; Silva, Ciro F. da

doi:10.1590/S0004-282X1988000100003

Abstracts

A freeze-fracture analysis of the satellite cells of spinal ganglia of the chick embryo was performed in 8 successive stages of development, from the 5th incubation day to hatching. The characteristic laminar disposition of the cells were first observed on the 7th day. Tight junctions were found at the 20th incubation day. Small groups or irregular aggregates of particles, but not gap junctions, were described on the 7th and 8th days. Pinocytotic vesicles were pointed out in the different stages considered.

Foi feita análise pelo método da crio-fratura de células satélites em 8 diferentes fases do desenvolvimento do embrião de galinha, do quinto dia à eclosão. A disposição laminar característica das células foi observada inicialmente no sétimo dia. Foram encontradas junções do tipo «tight» no 20º dia de incubação. Pequenos grupos ou agregados de partículas foram descritos no sétimo e oitavo dias, mas não típicas junções «gap». Vesículas de pinocitose foram apontadas nos diferentes estágios considerados.

Claudio A. Ferraz de Carvalho^I; Ciro F. da Silva^II

^IFull Professor - Department of Anatomy, Institute of Biomedical Science, University of São Paulo

^IIAssistant Professor - Department of Anatomy, Institute of Biomedical Science, University of São Paulo

SUMMARY

A freeze-fracture analysis of the satellite cells of spinal ganglia of the chick embryo was performed in 8 successive stages of development, from the 5th incubation day to hatching. The characteristic laminar disposition of the cells were first observed on the 7th day. Tight junctions were found at the 20th incubation day. Small groups or irregular aggregates of particles, but not gap junctions, were described on the 7th and 8th days. Pinocytotic vesicles were pointed out in the different stages considered.

RESUMO

Foi feita análise pelo método da crio-fratura de células satélites em 8 diferentes fases do desenvolvimento do embrião de galinha, do quinto dia à eclosão. A disposição laminar característica das células foi observada inicialmente no sétimo dia. Foram encontradas junções do tipo «tight» no 20º dia de incubação. Pequenos grupos ou agregados de partículas foram descritos no sétimo e oitavo dias, mas não típicas junções «gap». Vesículas de pinocitose foram apontadas nos diferentes estágios considerados.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

This work was performed in the Department of Anatomy, Institute of Biomedical Science, University of São Paulo and supported by a grant from FAPESP (PB-86/3336-0) to C.A.F.C.

We thank Prof. Antonio Sesso for the preparation of the freeze-fracture replicas

Departamento de Anatomia, Instituto de Ciências Biomédicas, Universidade de São Paulo - Caixa Postal 4365 - 01051 - São Paulo, SP - Brasil.

1. Bennet MVL, Trinkaus JP - Electrical coupling between embryonic cells by way of extracellular space and specialized junctions. J Cell Biol 44:592, 1970.
2. Brightman M, Reese TS - Junctions between intimately apposed cell membranes in the vertebrate brain. J Cell Biol 40: 648, 1969.
3. Decker RS, Friend DS - Assembly of gap junctions during amphibian neurulation. J Cell Biol 62: 32, 1974.
4. Pannese E - The histogenesis of the spinal ganglia. Adv Anat Embryol Cell Biol 47: 6, 1974.
5. Pannese E, Luciano L, Iurato S, Reale E - Intercellular junctions and other membrane specializations in developing spinal ganglia: a freeze-fracture study. J Ultrastruct Res 60: 169, 1977.
6. Pannese E, Luciano L, Reale L - Intercellular junctions in developing spinal ganglia. Zoon 6:129, 1978.
7. Pannese E - The satellite cells of the sensory ganglia. Adv Anat Embryol Cell Biol 65:1, 1981.
8. Revel JP, Karnovsky MJ - Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. J Cell Biol 33:7, 1967.
9. Revel JP, Yip P, Chang LL - Cell junctions in the early chick embryo: a freeze-etch study. Dev Biol 35: 302, 1973.

A freeze-fracture study on the developing satellite cells of spinal ganglia in the chick embryo

Estudo com a técnica da crio-fratura das células satélites dos gânglios sensitivos do embrião de galinha

Publication Dates

Publication in this collection
22 June 2011
Date of issue
Mar 1988

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. Bennet MVL, Trinkaus JP - Electrical coupling between embryonic cells by way of extracellular space and specialized junctions. J Cell Biol 44:592, 1970.

[2] 2. Brightman M, Reese TS - Junctions between intimately apposed cell membranes in the vertebrate brain. J Cell Biol 40: 648, 1969.

[3] 3. Decker RS, Friend DS - Assembly of gap junctions during amphibian neurulation. J Cell Biol 62: 32, 1974.

[4] 4. Pannese E - The histogenesis of the spinal ganglia. Adv Anat Embryol Cell Biol 47: 6, 1974.

[5] 5. Pannese E, Luciano L, Iurato S, Reale E - Intercellular junctions and other membrane specializations in developing spinal ganglia: a freeze-fracture study. J Ultrastruct Res 60: 169, 1977.

[6] 6. Pannese E, Luciano L, Reale L - Intercellular junctions in developing spinal ganglia. Zoon 6:129, 1978.

[7] 7. Pannese E - The satellite cells of the sensory ganglia. Adv Anat Embryol Cell Biol 65:1, 1981.

[8] 8. Revel JP, Karnovsky MJ - Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. J Cell Biol 33:7, 1967.

[9] 9. Revel JP, Yip P, Chang LL - Cell junctions in the early chick embryo: a freeze-etch study. Dev Biol 35: 302, 1973.