Bulls |
Streptomycin and penicillin |
100 to 1000 μg/mL or IU/mL of each |
Sodium citrate-egg yolk |
4,5°C |
20 days |
Almquist et al. (1949)Almquist JO, Glantz PJ, Shaffers HE. The effect of a combination of Penicillin and Streptomycin upon the livability and bacterial content of bovine semen. J Dairy Sci. 1949;32(2):183-90. http://dx.doi.org/10.3168/jds.S0022-0302(49)92025-1. http://dx.doi.org/10.3168/jds.S0022-0302...
|
Streptomycin and penicillin (alone or combination) |
1,000 mg/mL and 1,000 IU/mL |
Sodium citrate-egg yolk |
- |
- |
Almquist (1951)Almquist JO. A comparison of Penicillin, Streptomycin and Sulfanilamide for improving the fertility of semen from bulls of low fertility. J Dairy Sci. 1951;34(8):819-22. http://dx.doi.org/10.3168/jds.S0022-0302(51)91787-0. http://dx.doi.org/10.3168/jds.S0022-0302...
|
Gentamicin, tylosin, lincomycin and spectinomycin |
500 μg/mL, 100 μg/mL, 300 μg/mL and 600 μg/mL |
Tris-citric acid |
-196 °C |
1 day |
Andrabi et al. (2001)Andrabi SMH, Ahmad N, Abbas A, Anzar M. Effect of two different antibiotic combinations on fertility of frozen buffalo and Sahiwal bull semen. Pak Vet J. 2001;21(4):166-9.
|
Ceftiofur and tylosin |
200 μg/mL and 100 μg/mL |
Bioxcell CSS I and II |
-145 °C |
7 days |
Gloria et al. (2014)Gloria A, Contri A, Wegher L, Vignola G, Dellamaria D, Carluccio A. The effects of antibiotic additions to extenders on fresh and frozen-thawed bull semen. Anim Reprod Sci. 2014;150(1-2):15-23. http://dx.doi.org/10.1016/j.anireprosci.2014.08.012. PMid:25239841. http://dx.doi.org/10.1016/j.anireprosci....
|
Ofloxacin |
100 μg/mL |
Bioxcell CSS I and II |
-145 °C |
7 days |
Gloria et al. (2014)Gloria A, Contri A, Wegher L, Vignola G, Dellamaria D, Carluccio A. The effects of antibiotic additions to extenders on fresh and frozen-thawed bull semen. Anim Reprod Sci. 2014;150(1-2):15-23. http://dx.doi.org/10.1016/j.anireprosci.2014.08.012. PMid:25239841. http://dx.doi.org/10.1016/j.anireprosci....
|
Buffaloes |
Gentamicin, tylosin, lincomycin and spectinomycin |
500 μg/mL, 100 μg/mL, 300 μg/mL and 600 μg/mL |
Skimmed milk based |
5 °C |
3 days |
Akhter et al. (2008)Akhter S, Ansari MS, Andrabi S, Ullah N, Qayyum M. Effect of antibiotics in extender on bacterial and spermatozoal quality of cooled buffalo (Bubalus bubalis) bull semen. Reprod Domest Anim. 2008;43(3):272-8. http://dx.doi.org/10.1111/j.1439-0531.2007.00890.x. PMid:18042206. http://dx.doi.org/10.1111/j.1439-0531.20...
|
Streptomycin and penicillin G |
1,000 mg/mL and 1,000 IU/mL |
Skimmed milk based |
5 °C |
3 days |
Akhter et al. (2008)Akhter S, Ansari MS, Andrabi S, Ullah N, Qayyum M. Effect of antibiotics in extender on bacterial and spermatozoal quality of cooled buffalo (Bubalus bubalis) bull semen. Reprod Domest Anim. 2008;43(3):272-8. http://dx.doi.org/10.1111/j.1439-0531.2007.00890.x. PMid:18042206. http://dx.doi.org/10.1111/j.1439-0531.20...
|
Ciprofloxacin |
600 μg/mL |
Tris-citric acid |
-196 °C |
- |
Akhter et al. (2013)Akhter S, Ansari MS, Rakha BA, Andrabi SMH, Qadeer S, Iqbal R, Ullah N. Efficiency of ciprofloxacin for bacterial control, post-thaw quality, and in vivo fertility of buffalo spermatozoa. Theriogenology. 2013;80(4):378-83. http://dx.doi.org/10.1016/j.theriogenology.2013.05.001. PMid:23746693. http://dx.doi.org/10.1016/j.theriogenolo...
|
Gentamicin, tylosin, lincomycin and spectinomycin |
500 μg/mL, 100 μg/mL, 300 μg/mL and 600 μg/mL |
Tris-citric acid |
-196 °C |
1 day |
Andrabi et al. (2016)Andrabi SMH, Khan LA, Shahab M. Isolation of bacteria in semen and evaluation of antibiotics in extender for cryopreservation of buffalo (Bubalus bubalis) bull spermatozoa. Andrologia. 2016;48(10):1166-74. http://dx.doi.org/10.1111/and.12555. PMid:26892806. http://dx.doi.org/10.1111/and.12555...
|
Rams |
Streptomycin and penicillin G |
1,000 mg/mL and 1,000 IU/mL |
Tris-glucose |
-196 °C |
- |
Moustacas et al. (2010)Moustacas VS, Xavier MN, Carvalho-Júnior CA, Costa EA, Henry M, Santos RL. Effect of extender supplementation with various antimicrobial agents on viability of Brucella ovis and Actinobacillus seminis in cryopreserved ovine semen. Theriogenology. 2010;74(8):1476-81. http://dx.doi.org/10.1016/j.theriogenology.2010.06.019. PMid:20708246. http://dx.doi.org/10.1016/j.theriogenolo...
|
Gentamicin |
250 µg/mL |
Tris-glucose |
-196 °C |
- |
Moustacas et al. (2010)Moustacas VS, Xavier MN, Carvalho-Júnior CA, Costa EA, Henry M, Santos RL. Effect of extender supplementation with various antimicrobial agents on viability of Brucella ovis and Actinobacillus seminis in cryopreserved ovine semen. Theriogenology. 2010;74(8):1476-81. http://dx.doi.org/10.1016/j.theriogenology.2010.06.019. PMid:20708246. http://dx.doi.org/10.1016/j.theriogenolo...
|
Cefdinir |
1.0 mg/mL |
Sodium citrate-fructose-egg yolk |
5 °C |
4 days |
Azawi and Ismaeel (2012)Azawi OI, Ismaeel MA. Influence of addition of different antibiotics in semen diluent on viable bacterial count and spermatozoal viability of Awassi ram semen. Vet World. 2012;5(2):75-9. http://dx.doi.org/10.5455/vetworld.2012.75-79. http://dx.doi.org/10.5455/vetworld.2012....
|
Cefoperazone sodium |
1.0 mg/mL |
Sodium citrate-fructose-egg yolk |
5 °C |
4 days |
Azawi and Ismaeel (2012)Azawi OI, Ismaeel MA. Influence of addition of different antibiotics in semen diluent on viable bacterial count and spermatozoal viability of Awassi ram semen. Vet World. 2012;5(2):75-9. http://dx.doi.org/10.5455/vetworld.2012.75-79. http://dx.doi.org/10.5455/vetworld.2012....
|
Boars |
Gentamicin |
- |
- |
18°C |
3 days |
Mazurová and Vinter (1991)Mazurová J, Vinter P. The effect of selected antibiotics on microorganisms contaminating boar ejaculate. Vet Med (Praha). 1991;36(4):213-23. PMid:1771724.
|
Gentamicin and florfenicol |
100 μg/mL and 100 μg/mL |
Biosolwens Plus |
15 °C |
10 days |
Bryła and Trzcinska (2015)Bryła M, Trzcinska M. Quality and fertilizing capacity of boar spermatozoa during liquid storage in extender supplemented with different antibiotics. Anim Reprod Sci. 2015;163:157-63. http://dx.doi.org/10.1016/j.anireprosci.2015.11.005. PMid:26586542. http://dx.doi.org/10.1016/j.anireprosci....
|
Gentamicin |
250 µg/mL |
Beltsvile Thowing Solution |
17 °C |
3 days |
Waberski et al. (2019)Waberski D, Luther A-M, Grünther B, Jäkel H, Henning H, Vogel C, Peralta W, Weitze KF. Sperm function in vitro and fertility after antibiotic-free, hypothermic storage of liquid preserved boar semen. Sci Rep. 2019;9(1):1-10. http://dx.doi.org/10.1038/s41598-019-51319-1. PMid:31611589. http://dx.doi.org/10.1038/s41598-019-513...
|
Gentamicin |
250 µg/mL |
Androstar Premium |
17 °C |
3 days |
Waberski et al. (2019)Waberski D, Luther A-M, Grünther B, Jäkel H, Henning H, Vogel C, Peralta W, Weitze KF. Sperm function in vitro and fertility after antibiotic-free, hypothermic storage of liquid preserved boar semen. Sci Rep. 2019;9(1):1-10. http://dx.doi.org/10.1038/s41598-019-51319-1. PMid:31611589. http://dx.doi.org/10.1038/s41598-019-513...
|
- |
- |
Androstar Premium
|
5 °C |
3 days |
Waberski et al. (2019)Waberski D, Luther A-M, Grünther B, Jäkel H, Henning H, Vogel C, Peralta W, Weitze KF. Sperm function in vitro and fertility after antibiotic-free, hypothermic storage of liquid preserved boar semen. Sci Rep. 2019;9(1):1-10. http://dx.doi.org/10.1038/s41598-019-51319-1. PMid:31611589. http://dx.doi.org/10.1038/s41598-019-513...
|
Stallions |
Amikacin |
2,500 µg/mL |
Tris-egg yolk |
4 °C |
7 days |
Arriola and Foote (1982)Arriola RH, Foote AJ. Effects of amikacin sulfate on the motility of stallion and bull spermatozoa at different temperatures and intervals of storage. J Anim Sci. 1982;54(6):1105-10. http://dx.doi.org/10.2527/jas1982.5461105x. PMid:7107529. http://dx.doi.org/10.2527/jas1982.546110...
|
Amikacin or Ticarcillin |
2,000 µg/mL |
Skim milk-glucose |
23 °C and 5 °C, respectively |
1h and 2 days, respectively |
Jasko et al. (1993)Jasko DJ, Bedford SJ, Cook NL, Mumford EL, Squires EL, Pickett BW. Effect of antibiotics on motion characteristics of cooled stallion spermatozoa. Theriogenology. 1993;40(5):885-93. http://dx.doi.org/10.1016/0093-691X(93)90356-A. http://dx.doi.org/10.1016/0093-691X(93)9...
|
Gentamicin or polimixin B |
100 µg/mL or 1,000 IU/mL, respectively |
Skim-milk glucose |
20 °C and 5 °C, respectively |
8h and 2 days, respectively |
Vaillancourt et al. (1993)Vaillancourt D, Guay P, Higgins R. The effectiveness of gentamicin or polymyxin B for the control of bacterial growth in equine semen stored at 20 degrees C or 5 degrees C for up to forty-eight hours. Can J Vet Res. 1993;57(4):277-80. PMid:8269366.
|
Penicillin G and amikacin or Ticarcillin-clavulanic acid or Ceftiofur |
1,000 IU/mL and 1,000 µg/mL or 1,000 µg/mL or 1,000 µg/mL |
Skim-milk glucose |
5 °C |
1 day |
Varner et al. (1998)Varner DD, Scanlan CM, Thompson JA, Brumbaugh GW, Blanchard TL, Carlton CM, Johnson L. Bacteriology of preserved stallion semen and antibiotics in semen extenders. Theriogenology. 1998;50(4):559-73. http://dx.doi.org/10.1016/S0093-691X(98)00161-7. PMid:10732147. http://dx.doi.org/10.1016/S0093-691X(98)...
|
Gentamicin |
250 µg/mL |
EquiPro©
|
15 °C |
4 days |
Price et al. (2008)Price S, Aurich J, Davies-Morel M, Aurich C. Effects of oxygen exposure and gentamicin on stallion semen stored at 5 and 15 ºC. Reprod Domest Anim. 2008;43(3):261-6. http://dx.doi.org/10.1111/j.1439-0531.2007.00888.x. PMid:18042208. http://dx.doi.org/10.1111/j.1439-0531.20...
|
- |
- |
EquiPro© * |
5 °C |
4 days |
Price et al. (2008)Price S, Aurich J, Davies-Morel M, Aurich C. Effects of oxygen exposure and gentamicin on stallion semen stored at 5 and 15 ºC. Reprod Domest Anim. 2008;43(3):261-6. http://dx.doi.org/10.1111/j.1439-0531.2007.00888.x. PMid:18042208. http://dx.doi.org/10.1111/j.1439-0531.20...
|
Cefquinome |
0.99 mg/mL |
EquiPro©
|
5 °C |
2 days |
Parlevliet et al. (2011)Parlevliet JM, Lynn JW, Paccamonti DL. The use of Cefquinome in equine semen extender. J Equine Vet Sci. 2011;31(3):139-42. http://dx.doi.org/10.1016/j.jevs.2010.12.015. http://dx.doi.org/10.1016/j.jevs.2010.12...
|
Ticarcillin-clavulanic acid |
0.5, 1.0 and 1.5 mg/mL |
INRA 96® ** |
5 °C |
3 days |
Olivieri et al. (2011)Olivieri BT, Love BC, Rezabek GB, Lamm CG, Varner DD, Payton ME, Holyoak GR. Effect of Antibiotic-containing Extenders on Taylorella equigenitalis Contaminated Semen. J Equine Vet Sci. 2011;31(11):655-60. http://dx.doi.org/10.1016/j.jevs.2011.04.002. http://dx.doi.org/10.1016/j.jevs.2011.04...
|
Penicillin G and amikacin |
As supplied by the manufacturer |
VDMZ |
5 °C |
3 days |
Olivieri et al. (2011)Olivieri BT, Love BC, Rezabek GB, Lamm CG, Varner DD, Payton ME, Holyoak GR. Effect of Antibiotic-containing Extenders on Taylorella equigenitalis Contaminated Semen. J Equine Vet Sci. 2011;31(11):655-60. http://dx.doi.org/10.1016/j.jevs.2011.04.002. http://dx.doi.org/10.1016/j.jevs.2011.04...
|
Penicillin G and gentamicin |
1,000 IU/mL and 1,000 mg/mL |
BotuSemen®
|
5 and 15 °C |
1 day |
Ramires Neto et al. (2015) |
Penicillin G and amikacin |
1,000 IU/mL and 1,000 mg/mL |
TAMU |
Fresh and 5 °C |
1 day |
Hernández-Avilés et al. (2018)Hernández-Avilés C, Serafini R, Love CC, Teague SR, LaCaze KA, Lawhon SD, Wu J, Blanchard TL, Varner DD. The effects of antibiotic type and extender storage method on sperm quality and antibacterial effectiveness in fresh and cooled-stored stallion semen. Theriogenology. 2018;122:23-9. http://dx.doi.org/10.1016/j.theriogenology.2018.08.022. PMid:30219312. http://dx.doi.org/10.1016/j.theriogenolo...
|
Meropenem |
1,000 mg/mL |
TAMU |
Fresh and 5 °C |
1 day |
Hernández-Avilés et al. (2018)Hernández-Avilés C, Serafini R, Love CC, Teague SR, LaCaze KA, Lawhon SD, Wu J, Blanchard TL, Varner DD. The effects of antibiotic type and extender storage method on sperm quality and antibacterial effectiveness in fresh and cooled-stored stallion semen. Theriogenology. 2018;122:23-9. http://dx.doi.org/10.1016/j.theriogenology.2018.08.022. PMid:30219312. http://dx.doi.org/10.1016/j.theriogenolo...
|
Penicillin G and amikacin |
1,000 IU/mL and 1,000 mg/mL |
INRA 96®
|
Fresh and 5 °C |
1 day |
Hernández-Avilés et al. (2019)Hernández-Avilés C, Love CC, Serafini R, Teague SR, Varner DD. Supplemental antibiotics in a commercial extender for stallion semen. J Equine Vet Sci. 2019;80:33-5. http://dx.doi.org/10.1016/j.jevs.2019.06.014. PMid:31443830. http://dx.doi.org/10.1016/j.jevs.2019.06...
|
Dogs |
Gentamicin, tylosin, lincomycin and spectinomycin |
250, 50, 150 and 300 μg/ml; 500, 100, 300 and 600 μg/ml; and 1000, 200, 600 and 1200 μg/ml |
Tris-citric acid-fructose-egg yolk |
5 °C |
3 days |
Becher et al. (2013)Becher AC, Spergser J, Aurich C, Zottler E, Aurich JE, Schäfer-Somi S. Cooled storage of canine semen: in vitro effects of different concentrations of an antibiotic combination on growth of mollicutes. Reprod Domest Anim. 2013;48(6):961-6. http://dx.doi.org/10.1111/rda.12194. PMid:23808480. http://dx.doi.org/10.1111/rda.12194...
|
Koala (Phascolarctos cinereus) |
Penicillin G and gentamicin |
1,000 IU/mL and 100 µg/mL |
PBS |
16 °C |
1 day |
Johnston et al. (1998)Johnston SD, O’Boyle D, Frost AJ, McGowan MR, Tribe A, Higgins D. Antibiotics for the preservation of koala (Phascolarctos cinereus) semen. Aust Vet J. 1998;76(5):335-8. http://dx.doi.org/10.1111/j.1751-0813.1998.tb12362.x. PMid:9631702. http://dx.doi.org/10.1111/j.1751-0813.19...
|
Collared peccaries (Pecari tajacu) |
Streptomycin and penicillin G |
1,000 mg/mL and 1,000 IU/mL |
Tris-citric acid-fructose-egg yolk |
5 °C |
1,5 days |
Santos et al. (2019b)Santos CS, Dantas MRT, Rebouças MCG, Silva AM, Praxedes ECG, Campos LB, Oliveira MF, Silva AR. Efeito da inclusão de penicilina-estreptomicina no diluente para refrigeração do sêmen de catetos (Pecari tajacu). In: Abstracts of the 23th Brazilian Congress of Animal Reproduction; 2019 May 15-17; Gramado, Brazil. Gramado: CBRA; 2019b. p. 708.
|
Gentamicin |
70 µg/mL |
Tris-citric acid-fructose-egg yolk |
5 °C |
1,5 days |
Santos et al. (2019a)Santos CS, Dantas MRT, Rebouças MCG, Silva AM, Praxedes ECG, Campos LB, Oliveira MF, Silva AR. Adição de gentamicina ao diluente reduz a carga bacteriana e não afeta a qualidade do sêmen refrigerado de catetos (Pecari tajacu). In: Abstracts of the 23th Congresso Brasileiro de Reprodução Animal; 2019 May 15-17; Gramado, Brazil. Gramado: CBRA; 2019a. p. 701.
|