CTFR-Bovine Embryonic Stem cells |
Bovine |
Day 7 blastocyst (in vitro) or isolated ICMs |
Primed state |
OCT4 and SOX2 |
Custom-made base medium, BSA, FGF2 and IWR1 |
2D colonies |
Teratomas |
- |
- |
Bogliotti et al. (2018)Bogliotti YS, Wu J, Vilarino M, Okamura D, Soto DA, Zhong C, Sakurai M, Sampaio RV, Suzuki K, Izpisua Belmonte JC, Ross PJ. Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts. Proc Natl Acad Sci USA. 2018;115(9):2090-5. http://doi.org/10.1073/pnas.1716161115. PMid:29440377. http://doi.org/10.1073/pnas.1716161115...
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NBFR-Bovine Embryonic Stem cells |
Day 7 blastocysts (in vitro) |
N2B27, BSA, FGF2 and IWR1 |
- |
Soto et al. (2021)Soto DA, Navarro M, Zheng C, Halstead MM, Zhou C, Guiltinan C, Wu J, Ross PJ. Simplification of culture conditions and feeder-free expansion of bovine embryonic stem cells. Sci Rep. 2021;11(1):11045. http://doi.org/10.1038/s41598-021-90422-0. PMid:34040070. http://doi.org/10.1038/s41598-021-90422-...
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Bovine Embryonic Stem Cells |
Bovine |
Isolated ICMs from day 5 embryos (in vitro) incubated with C3 and PD0325901 |
Primed state |
OCT4, SOX2, NANOG, CD9, SSEA4 and CD49f/ITGA6 |
mTeSR1 and IWR1. Once established, media was changed to NBFR |
2D colonies |
- |
Yes |
BMP4, SCF, EGF, LIF, CHIR990221 and Y-27632 |
Shirasawa et al. (2024)Shirasawa A, Hayashi M, Shono M, Ideta A, Yoshino T, Hayashi K. Efficient derivation of embryonic stem cells and primordial germ cell-like cells in cattle. J Reprod Dev. 2024;70(2):82-7. http://doi.org/10.1262/jrd.2023-087. PMid:38355134. http://doi.org/10.1262/jrd.2023-087...
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Pig Embryonic Stem Cells |
Porcine |
Hatched blastocysts (in vitro) |
Primed state |
OCT4, SOX2, NANOG, SSEA1, SSEA4, TRA-1-60, and TRA-1-81 |
DMEM, KSR, a lipid concentrate, FGF2, ActA, CHIR99021 and IWR1 |
2D colonies |
Teratomas |
- |
- |
Choi et al. (2019)Choi K-H, Lee D-K, Kim SW, Woo S-H, Kim D-Y, Lee C-K. Chemically defined media can maintain pig pluripotency network in vitro. Stem Cell Reports. 2019;13(1):221-34. http://doi.org/10.1016/j.stemcr.2019.05.028. PMid:31257130. http://doi.org/10.1016/j.stemcr.2019.05....
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Expanded Potential Stem Cells (EPSC) |
Bovine |
Early day 5 blastocyst (in vivo) |
Expanded potential state |
OCT4, SOX2, NANOG, and SALL4 |
mTESR1, IWR1 (or XAV-939), CHIR99021, WH-4-023 or A419259, Vitamin C, ActA and LIF |
3D colonies |
Chimeras and teratomas |
- |
- |
Zhao et al. (2021)Zhao L, Gao X, Zheng Y, Wang Z, Zhao G, Ren J, Zhang J, Wu J, Wu B, Chen Y, Sun W, Li Y, Su J, Ding Y, Gao Y, Liu M, Bai X, Sun L, Cao G, Tang F, Bao S, Liu P, Li X. Establishment of bovine expanded potential stem cells. Proc Natl Acad Sci USA. 2021;118(15):e2018505118. http://doi.org/10.1073/pnas.2018505118. PMid:33833056. http://doi.org/10.1073/pnas.2018505118...
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Porcine |
OCT4 and NANOG |
N2B27, FBS, CHIR99021, WH-4-023, XAV-939 (or IWR1), Vitamin C, ActA and LIF |
Yes |
BMP2, LIF, SCF and EGF |
Gao et al. (2019)Gao X, Nowak-Imialek M, Chen X, Chen D, Herrmann D, Ruan D, Chen ACH, Eckersley-Maslin MA, Ahmad S, Lee YL, Kobayashi T, Ryan D, Zhong J, Zhu J, Wu J, Lan G, Petkov S, Yang J, Antunes L, Campos LS, Fu B, Wang S, Yong Y, Wang X, Xue SG, Ge L, Liu Z, Huang Y, Nie T, Li P, Wu D, Pei D, Zhang Y, Lu L, Yang F, Kimber SJ, Reik W, Zou X, Shang Z, Lai L, Surani A, Tam PPL, Ahmed A, Yeung WSB, Teichmann SA, Niemann H, Liu P. Establishment of porcine and human expanded potential stem cells. Nat Cell Biol. 2019;21(6):687-99. http://doi.org/10.1038/s41556-019-0333-2. PMid:31160711. http://doi.org/10.1038/s41556-019-0333-2...
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Embryonic Disc Stem Cells (EDSC) |
Bovine |
Isolated ICMs from day 7-9 blastocysts (in vitro) |
Primed state |
OCT4, SOX2 and NANOG |
N2B27, ActA, FGF2 and XAV-939 |
2D colonies |
Teratomas |
- |
- |
Kinoshita et al. (2021)Kinoshita M, Kobayashi T, Planells B, Klisch D, Spindlow D, Masaki H, Bornelöv S, Stirparo GG, Matsunari H, Uchikura A, Lamas-Toranzo I, Nichols J, Nakauchi H, Nagashima H, Alberio R, Smith A. Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species. Development. 2021;148(23):dev199901. http://doi.org/10.1242/dev.199901. PMid:34874452. http://doi.org/10.1242/dev.199901...
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Porcine |
Epiblasts from bilaminar discs from day 11 embryos (in vivo) |
- |
Ovine |
Day 7 blastocysts (in vitro) |
- |
Pre-gastrulation Epiblast Stem Cells (pgEpiSC) |
Porcine |
Epiblasts from day 10 embryos (in vivo) |
Formative pluripotency |
OCT4, SOX2, NANOG, SSEA1, SSEA4, TRA-1-81, and TRA-1-60 |
N2B27, KSR, Vitamin C, CHIR99021, IWR1, WH-4-023, LIF, ActA and FGF2 |
3D colonies |
Teratomas |
- |
- |
Zhi et al. (2022)Zhi M, Zhang J, Tang Q, Yu D, Gao S, Gao D, Liu P, Guo J, Hai T, Gao J, Cao S, Zhao Z, Li C, Weng X, He M, Chen T, Wang Y, Long K, Jiao D, Li G, Zhang J, Liu Y, Lin Y, Pang D, Zhu Q, Chen N, Huang J, Chen X, Yao Y, Yang J, Xie Z, Huang X, Liu M, Zhang R, Li Q, Miao Y, Tian J, Huang X, Ouyang H, Liu B, Xie W, Zhou Q, Wei H, Liu Z, Zheng C, Li M, Han J. Generation and characterization of stable pig pregastrulation epiblast stem cell lines. Cell Res. 2022;32(4):383-400. http://doi.org/10.1038/s41422-021-00592-9. PMid:34848870. http://doi.org/10.1038/s41422-021-00592-...
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Sheep Embryonic Stem Cells |
Ovine |
ICMs from day 7 blastocyst (in vitro) |
Primed state |
OCT4, SOX2 and NANOG |
Custom-made base medium, BSA, FGF2 and IWR1 |
2D colonies |
Teratomas |
- |
- |
Vilarino et al. (2020)Vilarino M, Soto DA, Bogliotti YS, Yu L, Zhang Y, Wang C, Paulson E, Zhong C, Jin M, Belmonte JCI, Wu J, Ross PJ. Derivation of sheep embryonic stem cells under optimized conditions. Reproduction. 2020;160(5):761-72. http://doi.org/10.1530/REP-19-0606. PMid:33065542. http://doi.org/10.1530/REP-19-0606...
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Chimera and Primordial Germ Cell Dual-Competent Pluripotent Stem Cells (XPSC) |
Equine |
Zona-free day 7 blastocyst (in vitro) |
Formative pluripotency |
OCT4 and SOX2 |
N2B27, FGF2, ActA and CHIR99021 |
2D colonies |
Chimeras and teratomas |
Yes |
BMP4, LIF, SCF and EGF |
Yu et al. (2021)Yu L, Wei Y, Sun HX, Mahdi AK, Pinzon Arteaga CA, Sakurai M, Schmitz DA, Zheng C, Ballard ED, Li J, Tanaka N, Kohara A, Okamura D, Mutto AA, Gu Y, Ross PJ, Wu J. Derivation of intermediate pluripotent stem cells amenable to primordial germ cell specification. Cell Stem Cell. 2021;28(3):550-67.e12. http://doi.org/10.1016/j.stem.2020.11.003. PMid:33271070. http://doi.org/10.1016/j.stem.2020.11.00...
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Rabbit Pluripotent Stem Cells |
Rabbit |
Day 6 epiblast, isolated day 4 ICMs or whole day 4 blastocysts (in vivo) |
Primed state |
OCT4, SOX2 and NANOG |
Essential 8, IWP2 and XAV-939 |
2D colonies |
Teratomas |
Yes |
B27, BMP4, LIF, SCF and EGF |
Kobayashi et al. (2021)Kobayashi T, Castillo-Venzor A, Penfold CA, Morgan M, Mizuno N, Tang WWC, Osada Y, Hirao M, Yoshida F, Sato H, Nakauchi H, Hirabayashi M, Surani MA. Tracing the emergence of primordial germ cells from bilaminar disc rabbit embryos and pluripotent stem cells. Cell Rep. 2021;37(2):109812. http://doi.org/10.1016/j.celrep.2021.109812. PMid:34644585. http://doi.org/10.1016/j.celrep.2021.109...
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