The effects of feeding graded levels of whole cottonseed on semen characteristics and testicular profiles of Red Sokoto Bucks

Itodo, Joy Iyojo; Ibrahim, Rekwot Peter; Rwuaan, Joseph Sankey; Aluwong, Tanga; Shiradiyi, Bugau John; Owoicho, Abah Kenneth; Azubuike, Ubah Simon; Agbi, Kuje Althea

doi:10.4025/actascianimsci.v43i1.50990

ABSTRACT.

The present work evaluated the effects of feeding graded levels of whole cottonseed on reproductive parameters of Red Sokoto bucks. Twenty Red Sokoto bucks were used for the experiment. After a 14-day pre-treatment period, bucks were assigned for 90 days to one of four isonitrogeneous treatments: control (diet A); 0 mg kg^-1 of total gossypol, (diet B); 15% mg kg^-1 of total gossypol, (diet C); 30% mg kg^-1 of total gossypol and (diet D); 45% mg kg^-1 of total gossypol. The mean percentage sperm gross motility was significantly (p < 0.05) lower in group D (45% WCS) compared to groups C (30% WCS) and A (control) at days 60, 75 and 90. The mean semen pH and reaction time were not significantly (p > 0.05) different among treatment groups. Semen colour of the bucks in the control group was majorly creamy, in group C (30% WCS) and B (15% WCS) creamy to milky and group D (45% WCS) colourless. The mean semen volume was significantly (p < 0.05) higher in groups C and A compared to group D (45% WCS) at days 60, 75 and 90. The mean semen concentration was significantly (p < 0.05) lower in group D (45% WCS) when compared to group A (control) at days 30, 45, 60, 75 and 90. Group A (control) bucks had significantly (p < 0.05) higher percentage live sperm compared to those in group D (45% WCS) at days 45, 60, 75 and 90. Mean Sperm morphological abnormalities including detached head, free tail curved tail and midpiece droplets were significantly (p < 0.05) higher in group D (45%) than in group A (Control) at day 15 (for free tails) and day 90 (for all). Testicular and epididymal sperm reserves were higher in animals supplemented with up to 30% whole cottonseed. In conclusion, feeding bucks above 30% WCS resulted in more deleterious effects on the semen characteristics and testicular profile.

Keywords:
bucks; gossypol; histology; semen; sperm reserves; testosterone concentration

[1] * Author for correspondence. E-mail: iyojojoy@gmail.com

Parameter	Day 0				Day 15
	A	B	C	D	A	B	C	D
Motility	82. 00± 4.64	76. 00±9.93	82.50±3.23	67. 00±3.74	91.00±1.81	81.0±6.2	78.0±1.23	66.0±10.89
Ph	6.4±0.24	6.8±0.37	7.8±0.37	7.2±0.37	6.8±0.20	6.8±0.20	6.60±0.24	7.0±0.45
Semen colour	Creamy	Creamy	Creamy	Creamy	Creamy	Creamy	Milky	Milky
Semen Volume	0.58±0.06	0.50±0.07	0.46±0.05	0.56±0.07	0.54±0.02	0.50±0.03	0.52±0.07	0.57±0.03
Concentration	437.5±65.24	403.8±117.0	505.0±90.0	363.3±108.5	525.8±66.49	544.7±171.3	510.8±32.78	657.3±17.6
Reaction Time	51.80± 16.91	58.20 ±12.14	51.00±8.58	61.60±10.11	32.25±6.09	39.60±11.10	26.50±5.95	36.40±2.1
Live sperm %	81.00±4.00	88.00±2.00	85.00±4.47	84.00±4.0	82.00±3.74	84.0±4.30	83.0±4.36	80.0±3.16
Dead sperm %	16.25±3.75	12.00±2.00	15.00±4.47	16.10±4.0	18.00±2.89	16.0±4.30	17.0±4.36	20.0±3.16
Detached head	15.67±3.38	12.80±2.48	16.00±3.11	17.00±1.87	13.60±2.11	14.50±3.75	15.20±4.07	16.40±1.97
Free Tail	10.60±2.04	9.60±2.36	7.4±1.17	13.0±0.84	3.25±1.11^a	8.0±2.03	4.80±0.9	10.00±1.82^a
Curve Tail	2.60±0.60	5.60±0.75	4.40±1.44	4.40±1.33	1.75±0.75	4.25±1.11	1.67±0.33	4.60±0.87
Bent Tail	3.75±0.48	8.00±1.08	6.00±1.73	6.80±1.32	4.40±1.47	5.80±2.04	6.25±1.70	7.50±2.63
Mid -piece	0 ± 0	0 ± 0	0 ± 0	0 ± 0	0.40±0.24	0.60±0.40	0.40±0.24	0.0±0.0
	Day 30				Day 45
Motility	65.50±16.01	58.75±13.90	78.33±6.0	42.50±2.50	82.50±7.50	60.00±11.73	68.33±4.41	41.67±13.02
pH	8.0±0.58	8.20±0.80	7.67±0.33	8.40±0.40	6.5±0.64	7.6±0.40	7.0±0.58	7.0±0.32
Semen Colour	Creamy	Creamy	Milky	Milky	Creamy	Milky	Milky	Watery
Semen Volume	0.50±0.040	0.46±0.02	0.17±0.033	0.30±0.0	0.85±0.41	0.35±0.15	0.33±0.12	0.33±0.10
Concentration	373±9.0^a	321.5±87.5^b	192.3±29.42	31.0±9.00^ab	373.0±9.0^a	321.5±87.50^b	192.3±29.42	31.0±9.00^ab
Reaction Time	78.25±17.57	83.20±28.02	83.33±6.67	142.5±23.27	44.50±13.77	20.60±7.42	45.00±14.00	36.40±5.49
Live sperm %	85.00±7.64	77.50±12.50	71.67±6.0	42.5±22.5	90.0±2.04^a	85.00±4.74^b	93.33±1.67^c	30.00±7.15^abc
Dead sperm %	15.00±10.93	22.50±12.50	30.00±4.56	57.5±22.50	10.00±2.04^a	15.00±4.74^b	16.67±4.41^c	70.00±7.15^abc
Detached head	14.75±9.67	15.67±8.0	10.50±5.24	15.00±2.89	10.33±4.41	19.20±7.07	7.33±3.71	19.20±7.20
Free Tail	3.75±2.50	3.67±1.76	5.25±2. 06	8.50±1.50	3.75±0.95	2.40±0.75	2.67±0.88	4.75±1.65
Curve Tail	2.0±0.82	2.0±0.82	3.40±1.40	7.50±2.50	3.50±0.65	3.20±1.28	2.67±0.67	5.80±1.59
Bent Tail	2.50±1.66	4.25±0.25	4.00±1.70	4.33±0.88	2.25±0.85	2.40±0.75	2.0±1.15	3.60±0.81
Mid-piece	0.0±0.0	0.0 ± 0.0	0.0 ± 0.0	0.2 ± 0.2	0.0±0.0	0.0 ± 0.0	0.0 ± 0.0	1.2 ± 0.58
	Day 60				Day 75
Motility	78.75±2.39^a	88.00±2.55^b	73.33±6.0^c	37.00±6.63^abc	72.75±1.09^a	69.00±2.56^b	65.33±6.1^c	32.00±7.12^abc
Ph	6.50±0.65	7.60±0.40	7.0±0.58	7.0±0.32	6.80±0.65	7.60±0.40	7.2±0.58	7.2±0.32
Semen Colour	Creamy	Creamy	Creamy	Watery	Creamy	Creamy	Milky	Watery
Semen Volume	0.40±0.04^a	0.66±0.06^b	0.50±0.15^c	0.12±0.04^abc	0.80±0.06^a	0.56±0.01^b	0.60±0.16^c	0.14±0.05^abc
Concentration	219.7±49.36	380.0±77.8^a	245.3±52.26^b	15.00±4.12^ab	380.7±76.04 ^a	245.0±82.10^b	291.3±42.56^c	18.30±4.32^abc
Reaction Time	59.0±12.20	76.80±15.39	84.67±35.88	74.80±11.51	59.0±12.20	76.80±15.39	74.67±35.88	84.80±11.51
Live sperm %	81.67±4.41^a	91.60±2.25^b	84.33±4.70	36.40±4.74^abc	90.67±4.41^a	82.60±2.25^b	70.33±4.70^c	36.40±4.74^abc
Dead sperm %	18.33±4.41^a	8.40±2.25^b	15.67±4.70^c	63.60±4.74^abc	10.33±4.41^a	18.40±2.25^b	30.67±4.70^c	64.60±4.74^abc
Detached head	7.75±1.55	8.20±3.31	6.33±1.45	12.80±2.85	7.75±1.55	8.20±3.31	6.33±1.45	12.80±2.85
Free Tail	3.50±0.96	5.60±2.11	4.0±1.15	5.7±0.88	3.50±0.96	5.60±2.11	4.0±1.15	6.7±0.88
Curve Tail	5.67±2.03	3.75±1.70	8.0 ± 0.0	7.0±1.52	3.67±2.03	3.75±1.70	7.0±0.0	7.0±1.52
Bent Tail	0.75±0.25^a	0.60±0.40^b	0.50±0.50	5.80±1.83^ab	0.45±0.15^a	0.50±0.80^b	0.55±0.50	6.20±1.^53ab
Mid-piece	0.0±0.0	0.20 ± 0.20	0.67 ± 0.67	2.0 ± 0.71	0.0±0.0	0.20 ± 0.20	0.47 ± 0.67	2.0 ± 0.71
Day 45	Day 90
Motility	80.00±2.04^a	65.00±2.24^b	55.02.±2.89^c	37.00±6.63^a
Ph	6.75±0.75	7.60±0.40	7.0±0.58	7.00±0.32
Semen Colour	Milky	Milky	Milky	Watery
Semen Volume	0.70±0.04^ab	0.66±0.06^cd	0.33±0.033^ac	0.14±0.02^bd
Concentration	125.6±9.8^ade	219.7±30.61^abc	54.67±16.37^bd	14.40±4.62^ce
Reaction Time	59.0±12.20	76.80±15.39	84.67±35.88	78.80±11.51
Live %	76.25±6.25^a	91.60±2.25^bc	65.00±2.89^bd	36.40±4.74^acd
Dead %	23.75±6.25^a	8.40±2.25^bc	35.00±2.89^bd	63.60±4.74^ac
Detached head	7.75±1.55^a	6.40±1.63^b	18.0±3.06	30.80±4.68^ab
Free Tail	3.50±0.96^a	5.60±2.11^b	12.67±4.41	17.50±1.04^ab
Curve Tail	3.25±0.75^ab	8.40±1.03	14.33±1.86^a	15.00±2.88^b
Bent Tail	0.75±0.25^a	0.60±0.40^b	0.50±0.50^c	7.80±1.59^abc
Mid-piece	0.0±0.0^a	0.20 ± 0.20^b	1.00 ± 0.58	2.20 ± 0.58^ab

Brasil

Brasil

The effects of feeding graded levels of whole cottonseed on semen characteristics and testicular profiles of Red Sokoto Bucks

ABSTRACT.