GC-MS Analysis and Bioactivity of Streptomyces sp. nkm1 Volatile Metabolites against some Phytopathogenic Fungi

Abstract

The main concern of today's agricultural production around the world is a cleaner and greener approach to crop production. Streptomyces species with distinct characteristics can be found in soil. The current research focuses on Streptomyces species isolated from marine sediment samples. To evaluate the morphology and biochemical properties of the isolated Streptomyces strain ukm1, it was grown in Starch Casein Nitrate (SCN) agar medium. Morphological, biochemical, and 16s rRNA analysis were performed on the strain. Streptomyces sp. was identified based on these findings. The production of bioactive metabolites by strain nkm1 was carried out in five different fermentative media. After 10 days of incubation, M6 medium was found to be efficient and was extracted with ethyl acetate. The MIC values revealed that the ethyl acetate extract inhibited the growth of plant pathogenic fungi such as Phytophthora palmivora (31.25 g/ml), Aspergillus flavus (15.62 g/ml), Rhizoctonia solani (31.25 g/ml), and Cladosporium herbarum (31.25 g/ml). The GC-MS analysis of the ethyl acetate extract revealed 26 components, the major volatile bioactive compound being nonadecane.

Keywords:
Streptomyces sp; ethyl acetate; plant pathogen; nonadecane

HIGHLIGHTS

• Streptomyces sp. nkm1 was isolated from a marine soil sample and identified using morphological and molecular techniques.

• Streptomyces sp. nkm1 had the highest antifungal activity against some plant pathogenic fungi on M6 medium.

• On Aspergillus flavus, the minimum inhibitor concentration of ethyl acetate Crude extract of Streptomyces sp. nkm1 was more effective.

• The GC-MS analysis of the ethyl acetate extract revealed 26 components, with nonadecane being the most important bioactive compound.