The antifungal activity of Penicillium steckii (MF1) against pathogenic fungi of rose causing verticillium wilt was evaluated under differential nutrient condition. A suitable and modified medium was obtained in which test fungus exhibited enhanced antifungal activity and produced larger inhibition zone against the verticillium fungus. The study carried out to modify the medium components at level of nitrogen, carbon source and their concentration on agar plates. Addition of amino acids, metals and plant growth hormones did not show positive effect but glycerol enhanced the antifungal activity very much.
antifungal; Penicillium; nutrient; rose; Verticillium wilt
