Down-Regulation of NFkB, Bax,TGF-β, Smad-2mRNA expression in the Livers of Carbon Tetrachloride Treated Rats using Different Natural Antioxidants

Al-Rasheed, Nouf Mohamed; Fadda, Laila Mohamed; Al-Rasheed, Nawal Mohamed; Ali, Hanaa Mahmoud; Yacoub, Hazar Ibrahim

doi:10.1590/1678-4324-2016150553

Acessibilidade / Reportar erro

Brasil

Brazilian Archives of Biology and Technology

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Biological and Applied Sciences • Braz. arch. biol. technol. 59 • 2016 • https://doi.org/10.1590/1678-4324-2016150553 copy

Down-Regulation of NFkB, Bax,TGF-β, Smad-2mRNA expression in the Livers of Carbon Tetrachloride Treated Rats using Different Natural Antioxidants

Authorship SCIMAGO INSTITUTIONS RANKINGS

The objective of this study is to examine whether silymarin alone or in combination with chlorogenic acid and/ or melatonin plays a modulatory role against apoptotic damage in rats liver induced by of CCl₄. The present work revealed that CCl₄ induced elevation of in Bax, Smad, TGF-β and NFkBhepatic mRNA expression, administration of silymarin alone down regulates these expressions. Treatment with chlorogenic acid and/ or melatonin along with silymarin produced best results in this concern. Bcl-2 expression was down regulated by CCl₄ whereas concurrent treatment of chlorogenic acid and/ or melatonin along with silymarin increased this expression. On conclusion, the use of chlorogenic acid and/ or melatonin potentiates the anti-apoptotic action of silymarin.

CCl₄; Silymarin; Chlorogenic acid; Melatonin; mRNA expression

Gene name	Primer sequence	Primer size (bp)
Refer-actin	Forward 5′ GAGACCTTCAACACCCCAGC 3′ Reverse 5′ ATGTCACGCACGATTTCCC 3′	263
NFkB	Forward 5′ CATGAAGAGAAGACACTGACCATGGAAA3′ Reverse 5′ TGGATAGAGGCTAAGTGT AGACACG 3′	329
Bax	Forward 5′ GTTGCCCTCTTCTACTTTG 3′ Reverse 5′ AGCCACCCTGGTCTTG 3′	194
Bcl-2	Forward 5′CGGGAGAACAGGGTATGA 3′ Reverse 5′ CAGGCTGGAAGGAGAAGAT 3′	224
TGF-β1	Forward 5′ TGCTAATGGTGGACCGCAA 3′ Reverse 5′ CACTGCTTCCCGAATGTCTGA 3′	329
Smad-2	Forward 5′ TCTCCGGCTGAACTGTCTCCTA 3′ Reverse 5′ GCGATTGAACACCAGAATGCA 3′	267

	Serum	Hepatic mRNA expression
	ALT (U\L)	NFkB	Bax	Bcl2	TGF-β	Smad-2
Control	48.29 ± 3.12	1.07± 0.1	1.11± 0.08	1.26± 0.15	1.09± 0.09	1.4± 0.51
CCl₄	107 ± 16.29 ^***a	12.2± 1.17 ^***a	14.56± 0.68 ^***a	0.23± 0.2 ^***a	11.6± 0.91 ^***a	11.93± 1.45 ^***a
Sil	74.5 ± 7.80 ^*b/a	7.73±0.37 ^***ab	9.4± 0.55 ^***ab	0.34± .0.03^***a	9.03± 0.2 ^***ab	7.8± 0.5 ^***ab
Sil + CGA	68 ± 9.41 ^**b/a	5.54± 0.46 ^***ab	6.36± 1.06 ^***ab	0.7± 0.13 ^***ab	7.14±1.1 ^***ab	5.97± 0.15 ^***ab
Sil + Mel	65± 5.81 ^**b/a	3.8± 0.4 ^***ab	5.2± 0.26 ^***ab	0.67± 0.02 ^***ab	5.23± 0.24 ^***ab	4.16±0.35 ^***ab
Sil+ CGA+ Mel	55.4± 5.09 ^***b	1.9±0.34 ^***b	2± 0.2 ^***b	0.95± 0.07 ^*b/a	3.26± 0.21 ^***ab	2.37± 0.57 ^***b

Instituto de Tecnologia do Paraná - Tecpar Rua Prof. Algacyr Munhoz Mader, 3775 - CIC, 81350-010 Curitiba PR Brazil, Tel.: +55 41 3316-3052/3054, Fax: +55 41 3346-2872 - Curitiba - PR - Brazil
E-mail: babt@tecpar.br

Acompanhe os números deste periódico no seu leitor de RSS

[TFN01] F: Forward primer sequence R: Reverse primer sequence