Demineralized human dentin matrix for alveolar ridge preservation using a volumetric and histologic analyses in rats

The aim of this study was to evaluate a Demineralized Human Dentine Matrix (DHDM) as viable biomaterial for alveolar ridge preservation in a rat model. Wistar rats were submitted to the extraction of maxillary first molars bilaterally. Sockets were filled with biomaterials and divided into 4 experimental groups (n=5): blood clot, autogenous bone, bovine-derived xenograft (BDX) and DHDM. Animals were sacrificed at 7, 14 e 28 days. Microtomography (uCT) volumetric evaluation and qualitative histological analyses were performed. Results obtained through the uCT showed similar values between the DHDM and the other experimental groups. The histological evaluation demonstrated DHDM with an unspecific inflammatory process and bone neoformation with slow reabsorption of the material. This result indicates that DHDM implanted in rat sockets is biocompatible and reduces the alveolar ridge volume loss after tooth extraction.

Key Words:
Demineralized human dentin matrix; biomaterial; biocompatibility; alveolar ridge preservation; bone substitutes

Authorship

Gabriela Fracasso Moraes

Post graduate Program in Dentistry, Department of Stomatology, Universidade Federal do Paraná, UFPR, Curitiba, BrazilUniversidade Federal do ParanáBrazilCuritiba, Brazil Post graduate Program in Dentistry, Department of Stomatology, Universidade Federal do Paraná, UFPR, Curitiba, Brazil

https://orcid.org/0000-0001-5588-8623

Rodrigo de Oliveira Caetano

https://orcid.org/0000-0001-6486-9344

Fernanda Harumi Oku Prochnow

https://orcid.org/0000-0003-1496-4495

Yasmine Mendes Pupo

Post graduate Program in Dentistry, Department of Restorative Dentistry, Universidade Federal do Paraná, UFPR, Curitiba, BrazilUniversidade Federal do ParanáBrazilCuritiba, Brazil Post graduate Program in Dentistry, Department of Restorative Dentistry, Universidade Federal do Paraná, UFPR, Curitiba, Brazil

https://orcid.org/0000-0003-4755-7191

Juliana Lucena Schussel

https://orcid.org/0000-0001-5204-0782

Humberto Osvaldo Schwartz-Filho

https://orcid.org/0000-0002-3873-4945

Correspondence: Professor Humberto Osvaldo Schwartz-Filho, Department of Stomatology, Universidade Federal do Paraná, UFPR; Rua Prefeito Lothário Meissner 632, 80210-170 Jardim Botânico,Curitiba - PR; Brazil; Phone: + 55 41 99804 0003 E-mail: betoschwartz@ufpr.br

SCIMAGO INSTITUTIONS RANKINGS

Figures

Figures (9)

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Figure 1
Scanning Electron Microscopy images of the Demineralized Human Dentine Matrix (DHDM). A) panoramic view of the DHDM particles (50x magnification) showing size homogeneity; B) approximate view of the particles (300x magnification), showing their irregular shape; C) photomicrograph of a DHDM particle (500x magnification). Red arrows show the transversely exposed dentinal tubules exposed transversely and yellow arrows show dentinal tubules exposed longitudinally; D) photomicrograph of a DHDM particle (1000x magnification). Red arrows show the transversely exposed dentinal tubules and yellow arrows show dentinal tubules exposed longitudinally and E) photomicrograph of the DHDM surface at magnification of 5000x.

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Figure 2
Bone volume (BV) (mm³) for all groups (blood clot, autogenous bone, bovine-derived xenograft [BDX] and Demineralized Human Dentine Matrix [DHDM]) evaluated at 07, 14 and 28 days. The bars represent the mean ± standard deviation of BV. p> 0.05 and 95% significance level (Kruskal-Wallis, Dunn's multiple comparison post-test, p <0.05). * Represent statistically significant differences between groups.

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Figure 3
Percentage of bone volume (BV/TV) (%) for all groups (blood clot, autogenous bone, bovine-derived xenograft [BDX] and Demineralized Human Dentine Matrix [DHDM]) evaluated at 07, 14 and 28 days. The bars represent the mean ± standard deviation of BV/TV. p> 0.05 and 95% significance level (Kruskal-Wallis, Dunn's multiple comparison post-test, p <0.05). * Represent statistically significant differences between groups.

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Figure 4
Bone surface (BS) (mm²) (%) for all groups (blood clot, autogenous bone, bovine-derived xenograft [BDX] and Demineralized Human Dentine Matrix [DHDM]) evaluated at 07, 14 and 28 days. The bars represent the mean ± standard deviation of BS. p> 0.05 and 95% significance level (Kruskal-Wallis, Dunn's multiple comparison post-test, p <0.05). No statistically significant results for the evaluated groups.

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Figure 5
Histological sections of socket repair after extraction for the period of 7 days. A) blood clot; B) autogenous bone; C) bovine-derived xenograft (BDX) and D) Demineralized Human Dentine Matrix (DHDM).

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Figure 6
Histological section of the Demineralized Human Dentine Matrix (DHDM) for the period of 7 days. It is possible to observe mineralized dental tissue in the DHDM (Arrow).

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Figure 7
Histological sections of socket repair after extraction for the period of 14 days. A) blood clot; B) autogenous bone; C) bovine-derived xenograft (BDX) and D) Demineralized Human Dentine Matrix (DHDM).

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Figure 8
Histological sections of socket repair after extraction for the period of 28 days. A) blood clot, B) autogenous bone, C) bovine-derived xenograft (BDX) and D) Demineralized Human Dentine Matrix (DHDM).

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Figure 9
Histological sections of socket repair after extraction for the period of 28 days. A) Moderate inflammatory infiltrate in bovine-derived xenograft (BDX) within 28 days (Arrow); B) Dentin remnants in Demineralized Human Dentine Matrix (DHDM) (Arrow).

Figure 1 Scanning Electron Microscopy images of the Demineralized Human Dentine Matrix (DHDM). A) panoramic view of the DHDM particles (50x magnification) showing size homogeneity; B) approximate view of the particles (300x magnification), showing their irregular shape; C) photomicrograph of a DHDM particle (500x magnification). Red arrows show the transversely exposed dentinal tubules exposed transversely and yellow arrows show dentinal tubules exposed longitudinally; D) photomicrograph of a DHDM particle (1000x magnification). Red arrows show the transversely exposed dentinal tubules and yellow arrows show dentinal tubules exposed longitudinally and E) photomicrograph of the DHDM surface at magnification of 5000x.

Figure 2 Bone volume (BV) (mm³) for all groups (blood clot, autogenous bone, bovine-derived xenograft [BDX] and Demineralized Human Dentine Matrix [DHDM]) evaluated at 07, 14 and 28 days. The bars represent the mean ± standard deviation of BV. p> 0.05 and 95% significance level (Kruskal-Wallis, Dunn's multiple comparison post-test, p <0.05). * Represent statistically significant differences between groups.

Figure 3 Percentage of bone volume (BV/TV) (%) for all groups (blood clot, autogenous bone, bovine-derived xenograft [BDX] and Demineralized Human Dentine Matrix [DHDM]) evaluated at 07, 14 and 28 days. The bars represent the mean ± standard deviation of BV/TV. p> 0.05 and 95% significance level (Kruskal-Wallis, Dunn's multiple comparison post-test, p <0.05). * Represent statistically significant differences between groups.

Figure 4 Bone surface (BS) (mm²) (%) for all groups (blood clot, autogenous bone, bovine-derived xenograft [BDX] and Demineralized Human Dentine Matrix [DHDM]) evaluated at 07, 14 and 28 days. The bars represent the mean ± standard deviation of BS. p> 0.05 and 95% significance level (Kruskal-Wallis, Dunn's multiple comparison post-test, p <0.05). No statistically significant results for the evaluated groups.

Figure 5 Histological sections of socket repair after extraction for the period of 7 days. A) blood clot; B) autogenous bone; C) bovine-derived xenograft (BDX) and D) Demineralized Human Dentine Matrix (DHDM).

Figure 6 Histological section of the Demineralized Human Dentine Matrix (DHDM) for the period of 7 days. It is possible to observe mineralized dental tissue in the DHDM (Arrow).

Figure 7 Histological sections of socket repair after extraction for the period of 14 days. A) blood clot; B) autogenous bone; C) bovine-derived xenograft (BDX) and D) Demineralized Human Dentine Matrix (DHDM).

Figure 8 Histological sections of socket repair after extraction for the period of 28 days. A) blood clot, B) autogenous bone, C) bovine-derived xenograft (BDX) and D) Demineralized Human Dentine Matrix (DHDM).

Figure 9 Histological sections of socket repair after extraction for the period of 28 days. A) Moderate inflammatory infiltrate in bovine-derived xenograft (BDX) within 28 days (Arrow); B) Dentin remnants in Demineralized Human Dentine Matrix (DHDM) (Arrow).

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Demineralized human dentin matrix for alveolar ridge preservation using a volumetric and histologic analyses in rats

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[1] Correspondence: Professor Humberto Osvaldo Schwartz-Filho, Department of Stomatology, Universidade Federal do Paraná, UFPR; Rua Prefeito Lothário Meissner 632, 80210-170 Jardim Botânico,Curitiba - PR; Brazil; Phone: + 55 41 99804 0003 E-mail: betoschwartz@ufpr.br