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Nota • Bol. Inst. Oceanogr. 38 (1) • 1990 • https://doi.org/10.1590/S0373-55241990000100009   copiar

Sistemática bioquímica de Menticirrhus americanus e Menticirrhus littoralis (Teleostei: Perciformes: Sciaenidae)

Biochemical systematics of Menticirrhus americanus and Menticirrhus littoralis (Teleostei Perciformes: Sciaenidae)

Autoria SCIMAGO INSTITUTIONS RANKINGS

Resumos

Populações de duas espécies congenéricas de peixes cienídeos do Atlântico Sul-Oriental, Menticirrhus americanus e Menticirrhus littoralis, analisados eletroforeticamente para detectar a variabilidade isoenzimática, mostraram muito pouca variação genética e uma moderadamente alta similaridade em sua composição alélica. Um total de 22 alelos constituídos por 17 loci foram observados. Variabilidade interespecífica foi encontrada para os loci G6pdh-A, G6pdh-B, Hbdh-B, Mdh-B e Sod-A. Variação intraespecífica foi observada somente para Mdh-B em Aí. littoralis. A similaridade genética entre M. americanus e M. littoralis (INEI = 0,76) é congruente com o alto nível de similaridade morfológica mostrado por estas duas espécies congenéricas.

Sistemática bioquímica; Peixes; Menticirrhus americanus; Menticirrhus littoralis; Sciaenidae; Eletroforese; Isoenzimas; Costa sul

Populations of two congeneric species of southwestern Atlantic sciaenid fish, Menticirrhus americanus and Menticirrhus littoralis, analysed electrophoretically to detect isoenzyme variability, showed very little genetic variation and a moderately high similarity in their allelic composition. A total of 22 alleles encoded by 17 loci were observed. Interspecific variability were found for the loci G6pdh-A, G6pdh-B, Hbdh-B, Mdh-B and Sod-A. Intraspecific variation was observed only for Mdh-B in M. littoralis. The genetic similarity between M. americanus and M. littoralis (INEI+0.76) is congruent with the high level of morphological similarity showed by these two congeneric species.

Biochemical systematics; Fishes; Menticirrhus americanus; Menticirrhus littoralis; Sciaenidae; Electrophoresis; Isoenzymes; South coast

NOTA

Sistemática bioquímica de Menticirrhus americanus e Menticirrhus littoralis (Teleostei: Perciformes: Sciaenidae)

Biochemical systematics of Menticirrhus americanus and Menticirrhus littoralis (Teleostei Perciformes: Sciaenidae)

Vicente P. F. Cassano* (* ) Bolsista do CNPq. Department of Biology, University of California at Los Angeles, 405 Hilgard Avenue. Los Angeles, California, 90024 -1606, U.S.A ; José A. Levy

Laboratório de Bioquímica Marinha, Departamento de Química Fundação Universidade do Rio Grande (Caixa Postal 474, 96.200 Rio Grande, RS)

RESUMO

Populações de duas espécies congenéricas de peixes cienídeos do Atlântico Sul-Oriental, Menticirrhus americanus e Menticirrhus littoralis, analisados eletroforeticamente para detectar a variabilidade isoenzimática, mostraram muito pouca variação genética e uma moderadamente alta similaridade em sua composição alélica. Um total de 22 alelos constituídos por 17 loci foram observados. Variabilidade interespecífica foi encontrada para os loci G6pdh-A, G6pdh-B, Hbdh-B, Mdh-B e Sod-A. Variação intraespecífica foi observada somente para Mdh-B em Aí. littoralis. A similaridade genética entre M. americanus e M. littoralis (INEI = 0,76) é congruente com o alto nível de similaridade morfológica mostrado por estas duas espécies congenéricas.

Descritores: Sistemática bioquímica, Peixes, Menticirrhus americanus, Menticirrhus littoralis, Sciaenidae, Eletroforese, Isoenzimas, Costa sul: Brasil.

ABSTRACT

Populations of two congeneric species of southwestern Atlantic sciaenid fish, Menticirrhus americanus and Menticirrhus littoralis, analysed electrophoretically to detect isoenzyme variability, showed very little genetic variation and a moderately high similarity in their allelic composition. A total of 22 alleles encoded by 17 loci were observed. Interspecific variability were found for the loci G6pdh-A, G6pdh-B, Hbdh-B, Mdh-B and Sod-A. Intraspecific variation was observed only for Mdh-B in M. littoralis. The genetic similarity between M. americanus and M. littoralis (INEI+0.76) is congruent with the high level of morphological similarity showed by these two congeneric species.

Descriptors: Biochemical systematics, Fishes, Menticirrhus americanus, Menticirrhus littoralis, Sciaenidae, Electrophoresis, Isoenzymes, South coast: Brazil.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

Agradecimentos

Agradecemos a Leo Lacerda e Maria Regina Casartelli pela colaboração e à Dra Anna Kmília A. de M. Vazzoler e Dr. Antonio Sole-Cava pela leitura crítica e sugestões apresentadas. Este trabalho contou com o auxílio do CNPq (Proc. 40.5654/83) e FURG.

Referências bibliográficas

ALLENDORF, F. W. & UTTER, F. M. 1979. Population genetics. In: Hoar, S. et al, eds Fish physiology. New York, Academic Press, v.8, p.407-454.

AVISE, J. C 1977. Genetic differentiation during speciation. In: Ayala, F. J., ed. Molecular evolution. Sunderland, Sinauer Assoc p.106-122.

AYALA, F.J. 1983. Enzymes as taxonomic characteres. In: Oxford, G. S. & Rollinson, D., eds Protein polymorphism: adaptative and taxonomic significance. London, Academic Press. (Systematics Association Special Volume nº 24).

BREWER, G. J. 1970. An introduction to isozymes techniques. New York, Academic Press.

BUCKLIN, A. & HEDGECOCK, D. 1982. Biochemical genetic evidence for a third species of Metridium (Coelenterata: Actiniaria). Mar. Biol., 66:1-7.

BUTH, D. G. 1983. Duplicate isozyme loci in fishes: origins, distribution, phyletic consequences, and locus nomenclature. In: Isozymes. New York, Alan R. Liss. p. 381-400.

CHAO, L. N. 1978. A basis for classifying western Atlantic Sciaenidae (Teleostei: Perciformes). Tech. Rept natn. mar. Fish. Serv. U.S., Circ, (415): 1-64.

DOBZHANSKY, T. G.; AYALA, F. J.; STEBBINS, G. L. & VALENTINE, J. W. 1977. Evolution. San Francisco, W. H. Freeman.

FAO/UNEP. 1981. Conservation of the genetic resources of fish: problems and recommendations. Report of the Expert Consultation on the Genetic Resources of Fish. Rome, 9-13 June 1980. FAO Fish. tech. Pap., (217):1-43.

FERGUSON, A. & MASON, F. M. 1981. Allozyme evidence for reproductively isolated sympatric populations of brown trout Salmo trutta L. in Louch Melvin, Ireland. J. Fish Biol., 18:629-642.

GORMAN, G. C. & RENZI, J. 1979. Genetic distance and heterozygosity estimates in electrophoretic studies: effects of sample size. Copeia, (2):242-249.

GRANT, W.S. 1985. Biochemical genetic stock structure of the southern African enchovy, Engraulis capensis Gilchrist. J. Fish Biol., 27:23-29.

GYLLENSTEN, U. 1985. The genetic structure of fish: differences in the intraspecific distribution of biochemical genetic variation between marine, anadromous, and freshwater species. J. Fish Biol., 26:691-699.

HADFIELD, A. J.; IVANTSOFF, V. & JOHNSTON, P. G. 1979. Clinal variation in electrophoretic and morphological characters between two nominal species of the genus Pseudomugfl (Pisces: Atheriniformes: Pseudomugilidae). Aust. J. mar. Freshw. Res., 30:375-386.

HUDON, J. & GUDERLEY, H. 1984. An electrophoretic study of the phylogenetic relationships among four species of sticklebacks (Pisces: Gasterosteidae). Can. J. Zool., 62:2313-2316.

JARDIM, L. F. A. 1988. Sinopse das espécies de Menticirrhus Gill, 1861 (Osteichthyes, Sciaenidae) do Atlântico Ocidental. Revta bras. Zool., 5(2):179-187.

LUCOTTE, G. & LEFEBVRE, J. 1980. Distances electrophorétiques entre les cinq espèces de babouins du genre Papio basées sur les mobilités des protéines et enzimes seriques. Biochem. Syst. Ecol., 5:61-63.

MAYR E. 1970. Populations, species and evolution: an abridgement of animal species and evolution. Cambridge, Harvard University Press. 453p.

McANDREW, B. J.; WARD, R. D. & BEARDMORE, J. A. 1982. Lack of relationship between morphological variance and enzyme heterozygosity in the plaice Pieuronectes piatessa. Heredity, 48:117-125.

MENEZES, N. A. & FIGUEIREDO, J. L. 1980. Manual de peixes marinhos do sudeste do Brasil. IV. Teleostei. (3). São Paulo, Museu de Zoologia da Universidade de São Paulo. 96p.

NEI, M. 1972. Genetic distance between populations. Am. Naturalist, 106:283-291.

______ 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89:583-590.

POULIK, M. D. 1957. Starch gel electrophoresis in a discontinuous system of buffers. Nature, Lond., 180:1477-1479.

SHAW, C. R. & PRASAD, R. 1970. Starch gel electrophoresis of enzymes -a compilation of recipes. Biochem. Genetics, 4:297-320.

SOLE-CAVA, A. M.; VOOREN, C. M. & LEVY, J. A. 1983. Isozyme differentiation of two sibling species of Squatina (Chondrichthyes) in south Brazil. Comp. Biochem. Physiol, 75B:355-358.

THORPE, J. P. 1979. Enzyme variation and taxonomy: the estimation of sampling errors in measurements of interspecific genetic similarity. Biol. J. Linn. Soc., 11:369-386.

(Recebido em 02-02-89; aceito em 19-02-90)

  • ALLENDORF, F. W. & UTTER, F. M. 1979. Population genetics. In: Hoar, S. et al, eds Fish physiology. New York, Academic Press, v.8, p.407-454.
  • AVISE, J. C 1977. Genetic differentiation during speciation. In: Ayala, F. J., ed. Molecular evolution. Sunderland, Sinauer Assoc p.106-122.
  • AYALA, F.J. 1983. Enzymes as taxonomic characteres. In: Oxford, G. S. & Rollinson, D., eds Protein polymorphism: adaptative and taxonomic significance. London, Academic Press. (Systematics Association Special Volume nş 24).
  • BREWER, G. J. 1970. An introduction to isozymes techniques. New York, Academic Press.
  • BUCKLIN, A. & HEDGECOCK, D. 1982. Biochemical genetic evidence for a third species of Metridium (Coelenterata: Actiniaria). Mar. Biol., 66:1-7.
  • BUTH, D. G. 1983. Duplicate isozyme loci in fishes: origins, distribution, phyletic consequences, and locus nomenclature. In: Isozymes. New York, Alan R. Liss. p. 381-400.
  • CHAO, L. N. 1978. A basis for classifying western Atlantic Sciaenidae (Teleostei: Perciformes). Tech. Rept natn. mar. Fish. Serv. U.S., Circ, (415): 1-64.
  • DOBZHANSKY, T. G.; AYALA, F. J.; STEBBINS, G. L. & VALENTINE, J. W. 1977. Evolution. San Francisco, W. H. Freeman.
  • FAO/UNEP. 1981. Conservation of the genetic resources of fish: problems and recommendations. Report of the Expert Consultation on the Genetic Resources of Fish. Rome, 9-13 June 1980.
  • FAO Fish. tech. Pap., (217):1-43.
  • FERGUSON, A. & MASON, F. M. 1981. Allozyme evidence for reproductively isolated sympatric populations of brown trout Salmo trutta L. in Louch Melvin, Ireland. J. Fish Biol., 18:629-642.
  • GORMAN, G. C. & RENZI, J. 1979. Genetic distance and heterozygosity estimates in electrophoretic studies: effects of sample size. Copeia, (2):242-249.
  • GRANT, W.S. 1985. Biochemical genetic stock structure of the southern African enchovy, Engraulis capensis Gilchrist. J. Fish Biol., 27:23-29.
  • GYLLENSTEN, U. 1985. The genetic structure of fish: differences in the intraspecific distribution of biochemical genetic variation between marine, anadromous, and freshwater species. J. Fish Biol., 26:691-699.
  • HADFIELD, A. J.; IVANTSOFF, V. & JOHNSTON, P. G. 1979. Clinal variation in electrophoretic and morphological characters between two nominal species of the genus Pseudomugfl (Pisces: Atheriniformes: Pseudomugilidae). Aust. J. mar. Freshw. Res., 30:375-386.
  • HUDON, J. & GUDERLEY, H. 1984. An electrophoretic study of the phylogenetic relationships among four species of sticklebacks (Pisces: Gasterosteidae). Can. J. Zool., 62:2313-2316.
  • JARDIM, L. F. A. 1988. Sinopse das espécies de Menticirrhus Gill, 1861 (Osteichthyes, Sciaenidae) do Atlântico Ocidental. Revta bras. Zool., 5(2):179-187.
  • LUCOTTE, G. & LEFEBVRE, J. 1980. Distances electrophorétiques entre les cinq espèces de babouins du genre Papio basées sur les mobilités des protéines et enzimes seriques. Biochem. Syst. Ecol., 5:61-63.
  • MAYR E. 1970. Populations, species and evolution: an abridgement of animal species and evolution. Cambridge, Harvard University Press. 453p.
  • McANDREW, B. J.; WARD, R. D. & BEARDMORE, J. A. 1982. Lack of relationship between morphological variance and enzyme heterozygosity in the plaice Pieuronectes piatessa. Heredity, 48:117-125.
  • MENEZES, N. A. & FIGUEIREDO, J. L. 1980. Manual de peixes marinhos do sudeste do Brasil. IV. Teleostei. (3). São Paulo, Museu de Zoologia da Universidade de São Paulo. 96p.
  • NEI, M. 1972. Genetic distance between populations. Am. Naturalist, 106:283-291.
  • ______ 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89:583-590.
  • POULIK, M. D. 1957. Starch gel electrophoresis in a discontinuous system of buffers. Nature, Lond., 180:1477-1479.
  • SHAW, C. R. & PRASAD, R. 1970. Starch gel electrophoresis of enzymes -a compilation of recipes. Biochem. Genetics, 4:297-320.
  • SOLE-CAVA, A. M.; VOOREN, C. M. & LEVY, J. A. 1983. Isozyme differentiation of two sibling species of Squatina (Chondrichthyes) in south Brazil. Comp. Biochem. Physiol, 75B:355-358.
  • THORPE, J. P. 1979. Enzyme variation and taxonomy: the estimation of sampling errors in measurements of interspecific genetic similarity. Biol. J. Linn. Soc., 11:369-386.
  • (*
    ) Bolsista do CNPq. Department of Biology, University of California at Los Angeles, 405 Hilgard Avenue. Los Angeles, California, 90024 -1606, U.S.A

    • Datas de Publicação

    • Publicação nesta coleção
      18 Maio 2012
    • Data do Fascículo
      1990

    Histórico

    • Aceito
      19 Fev 1990
    • Recebido
      02 Fev 1989
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