Some observations on marine phytoplankton kinetics: 2. The effect of nitrate and ammonium concentrations on the growth and uptake rates of the natural population of Ubatuba region, SP (23ºS, 045ºW)

Schmidt, Gilda

doi:10.1590/S0373-55241983000100009

Abstracts

The phytoplankton Ks anã Vmax as a function of ammonium and nitrate concentrations were determined. The growth rate was estimated from measurements on synthesized chlorophyll-α and the cell number in the. culture media. The uptake rate was determined as to the consumption of ammonium and nitrate after the nutrients depletion from culture media.

Phytopiankton; Kinetics; Nitrates; Ammonium; Growth; Chlorophylls; Nutrients (mineral); Limiting factors; Cell counting; Fluorescence; Phaeodactylum tricornutum; Ubatuba; SP

Foram determinadas a Ks e a Vmax do fi toplancton natural, em função da concen tração de amonia e nitrato. A velocida de de crescimento foi determinada como clorofila-a sintetizada e numero de cé lulas no meio de cultura. A velocidade de assimilação foi determinada como o consumo de amonia e nitrato depois do esgotamento desses nutrientes do meio de cultura.

Fitoplâncton; Cinética; Nitratos; Amonio; Crescimento; Clorofilas; Nutrientes minerais; Fatores limitantes; Contagem de célula; Fluorescencia; Phaeodactylum tricornutum; Ubatuba; SP

ARTIGOS

Some observations on marine phytoplankton kinetics. 2. The effect of nitrate and ammonium concentrations on the growth and uptake rates of the natural population of Ubatuba region, SP (23ºS, 045ºW)

Gilda Schmidt

Instituto Oceanográfico da Universidade de São Paulo

SYNOPSIS

The phytoplankton Ks anã Vmax as a function of ammonium and nitrate concentrations were determined. The growth rate was estimated from measurements on synthesized chlorophyll-α and the cell number in the. culture media. The uptake rate was determined as to the consumption of ammonium and nitrate after the nutrients depletion from culture media.

Descriptors: Phytopiankton, Kinetics, Nitrates, Ammonium, Growth, Chlorophylls, Nutrients (mineral), Limiting factors, Cell counting, Fluorescence, Phaeodactylum tricornutum, Ubatuba, SP.

RESUMO

Foram determinadas a Ks e a Vmax do fi toplancton natural, em função da concen tração de amonia e nitrato. A velocida de de crescimento foi determinada como clorofila-a sintetizada e numero de cé lulas no meio de cultura. A velocidade de assimilação foi determinada como o consumo de amonia e nitrato depois do esgotamento desses nutrientes do meio de cultura.

Descritores: Fitoplâncton, Cinética, Nitratos, Amonio, Crescimento, Clorofilas, Nutrientes minerais, Fatores limitantes, Contagem de célula, Fluorescencia, Phaeodactylum tricornutum, Ubatuba, SP.

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(Received 14-Dec-1981; accepted 06-Oct-1983)

DROOP, M. R. 1974. The nutrient status of algal cells in continuous culture. J. mar. biol. Ass. U.K., 54:825-855.
DUGDALE, R. C. 1967. Nutrient limitation in the sea: dynamics, identification and significance. Limnol. Oceanogr., 12:685-695.
______ & GOERING, J. J. 1967. Uptake of new and regenerated forms of nitrogen in primary productivity. Limnol. Oceanogr., 72:196-206.
EPPLEY, R. W. & THOMAS, W. H. 1969. Comparison of half-saturation constants for growth and nitrate uptake of marine phytoplankton. J. Phycol., 5(4):375-379.
GOLDMAN, J. C; McCARTHY, J. J. & PEAVEY, D. G. 1979. Growth rate influence on the chemical compo sition of phytoplankton in oceanic waters. Nature, Lond., 279(5710): 210-215.
HASLE, R. G. 1978. Identification problems: general recommendations. In: Sournia, A. ed. - Phytoplankton manual. Monogr. Oceanogr. Methodol., (6):125-128.
McALLISTER, C. D.; SHAH, N. & STRICKLAND, J. D. H. 1964. Marine phytoplankton photosynthesis as a function of ligth intensity: a comparison of methods. J. Fish. Res. Bd Can., 21(1):159-181.
MacCARTHY, J. J.; TAYLOR, W. R. & TAFT, L. 1977. Nitrogenous nutrition of the plankton in the Chesapeake Bay. I. Nutrient availability and phytoplankton preferences. Limnol., Oceanogr., 22(6):996-1011.
MULLIN, J. B. & RILEY, J. P. 1955. The espectrophometric determination of nitrate in natural waters, with particular reference to sea-water. Analytica chim. Acta, 12:646-480.
PARSONS, T. R.; HARRISON, P.J. & WATERS, R. 1978. An experimental simulation of changes diatom and flagellates blooms. J. exp. mar. Biol. Ecol., 32:285-294.
SASSI, R. 1978. Variação sazonal do fitoplâncton e fatores ecológicos básicos da região do Saco da Ribeira (Lat. 23ş30'S, Long. 45ş07'W). Ubatuba, Brasil. Dissertação de mestrado. Universidade de São Paulo, Instituto Oceanográfico, 147p.
SCHMIDT, G. 1982. Algumas observações sobre a cinética do fitoplâncton marinho. 1. Influencia da concentração de nitrato e amónia na velocidade de crescimento e de assimilação desses nutrientes na diatomácea Phaedactylum tricornutum (Bohlin). Bolm Inst, oceanogr., S Paulo, 31(2): 13-27.
SOLORZANO, L. 1969, Determination of ammonia in natural waters by the phenolhypochlorite method. Limnol., Oceanogr., 14:799-801.
STRICKLAND, J. D. H. & PARSONS, T. R. 1968. A practical handbook of seawater analysis. Bull. Fish. Res. Bd Can., (167):1-311.
TEIXEIRA, C. & KUTNER, M. B. B. 1962. Plankton studies in a mangrove environment. I. First assessment of standing - stock and principal ecological factors. Bolm Inst, oceanogr., S Paulo, 12:101-124.
______ & TUNDISI, J. 1967. Primary production and phytoplankton in equatorial waters. Bull. "mar. Sci., 17(4):884-891.
THOMAS, H. W. 1970. On nitrogen deficiency in tropical Pacific oceanic phytoplankton: photosynthesis parameters in poor and rich water. Limnol. Oceanogr., 15(3):380-385.
______ & DODSON, A. N. 1972. Eutrophication in coastal waters: nitrogen as a controlling factor. Final Rep. U.S. Environm. Prot. Ag., Inst. mar. Resour., Univ. Cal. 67 p.
______ 1974. Effect of interactions between temperature and nitrate supply on the cell-division rates of two marine phytoflagellates. Mar. Biol., 24:213-217.

Publication Dates

Publication in this collection
06 June 2012
Date of issue
1983

History

Received
14 Dec 1981
Accepted
06 Oct 1983

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] DROOP, M. R. 1974. The nutrient status of algal cells in continuous culture. J. mar. biol. Ass. U.K., 54:825-855.

[2] DUGDALE, R. C. 1967. Nutrient limitation in the sea: dynamics, identification and significance. Limnol. Oceanogr., 12:685-695.

[3] ______ & GOERING, J. J. 1967. Uptake of new and regenerated forms of nitrogen in primary productivity. Limnol. Oceanogr., 72:196-206.

[4] EPPLEY, R. W. & THOMAS, W. H. 1969. Comparison of half-saturation constants for growth and nitrate uptake of marine phytoplankton. J. Phycol., 5(4):375-379.

[5] GOLDMAN, J. C; McCARTHY, J. J. & PEAVEY, D. G. 1979. Growth rate influence on the chemical compo sition of phytoplankton in oceanic waters. Nature, Lond., 279(5710): 210-215.

[6] HASLE, R. G. 1978. Identification problems: general recommendations. In: Sournia, A. ed. - Phytoplankton manual. Monogr. Oceanogr. Methodol., (6):125-128.

[7] McALLISTER, C. D.; SHAH, N. & STRICKLAND, J. D. H. 1964. Marine phytoplankton photosynthesis as a function of ligth intensity: a comparison of methods. J. Fish. Res. Bd Can., 21(1):159-181.

[8] MacCARTHY, J. J.; TAYLOR, W. R. & TAFT, L. 1977. Nitrogenous nutrition of the plankton in the Chesapeake Bay. I. Nutrient availability and phytoplankton preferences. Limnol., Oceanogr., 22(6):996-1011.

[9] MULLIN, J. B. & RILEY, J. P. 1955. The espectrophometric determination of nitrate in natural waters, with particular reference to sea-water. Analytica chim. Acta, 12:646-480.

[10] PARSONS, T. R.; HARRISON, P.J. & WATERS, R. 1978. An experimental simulation of changes diatom and flagellates blooms. J. exp. mar. Biol. Ecol., 32:285-294.

[11] SASSI, R. 1978. Variação sazonal do fitoplâncton e fatores ecológicos básicos da região do Saco da Ribeira (Lat. 23ş30'S, Long. 45ş07'W). Ubatuba, Brasil. Dissertação de mestrado. Universidade de São Paulo, Instituto Oceanográfico, 147p.

[12] SCHMIDT, G. 1982. Algumas observações sobre a cinética do fitoplâncton marinho. 1. Influencia da concentração de nitrato e amónia na velocidade de crescimento e de assimilação desses nutrientes na diatomácea Phaedactylum tricornutum (Bohlin). Bolm Inst, oceanogr., S Paulo, 31(2): 13-27.

[13] SOLORZANO, L. 1969, Determination of ammonia in natural waters by the phenolhypochlorite method. Limnol., Oceanogr., 14:799-801.

[14] STRICKLAND, J. D. H. & PARSONS, T. R. 1968. A practical handbook of seawater analysis. Bull. Fish. Res. Bd Can., (167):1-311.

[15] TEIXEIRA, C. & KUTNER, M. B. B. 1962. Plankton studies in a mangrove environment. I. First assessment of standing - stock and principal ecological factors. Bolm Inst, oceanogr., S Paulo, 12:101-124.

[16] ______ & TUNDISI, J. 1967. Primary production and phytoplankton in equatorial waters. Bull. "mar. Sci., 17(4):884-891.

[17] THOMAS, H. W. 1970. On nitrogen deficiency in tropical Pacific oceanic phytoplankton: photosynthesis parameters in poor and rich water. Limnol. Oceanogr., 15(3):380-385.

[18] ______ & DODSON, A. N. 1972. Eutrophication in coastal waters: nitrogen as a controlling factor. Final Rep. U.S. Environm. Prot. Ag., Inst. mar. Resour., Univ. Cal. 67 p.

[19] ______ 1974. Effect of interactions between temperature and nitrate supply on the cell-division rates of two marine phytoflagellates. Mar. Biol., 24:213-217.

Brasil

Brasil

Some observations on marine phytoplankton kinetics: 2. The effect of nitrate and ammonium concentrations on the growth and uptake rates of the natural population of Ubatuba region, SP (23ºS, 045ºW)

Abstracts

Publication Dates

History