A description is given of Dentostomina besnardii, new species, from Banco de São Tomé (Lat. 22º 08' S - Long 40º 39' W) and Trindade (Lat. 20º 33' S - Long. 29º 22' W), coast of Brazil. The species is similar to D. guraboensis Bermudez, 1949, differing from it by the position of the bifid tooth and the size of the grains which form the test.