Physiological and biochemical changes induced by Qiangdi nano-863 biological assistant growth apparatus during rice seed priming under temperature stress

Younas, A.; Xiukang, W.; Yousaf, Z.; Fiaz, S.; Riaz, A.; Hussain, S.; Huang, S.

doi:10.1590/1519-6984.245206

Brasil

home Sumário navigate_before Anterior Atual Seguinte navigate_next

home Sumário

Original Article • Braz. J. Biol. 83 • 2023 • https://doi.org/10.1590/1519-6984.245206 linkcopy

Physiological and biochemical changes induced by Qiangdi nano-863 biological assistant growth apparatus during rice seed priming under temperature stress

Alterações fisiológicas e bioquímicas induzidas pelo aparelho de crescimento assistente biológico Qiangdi nano-863 durante o priming de sementes de arroz sob estresse de temperatura

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

A huge amount of rice cultivation and consumption occur in Asia particularly in Pakistan and China. However, multiple abiotic stresses especially high and low-temperature proved to be a substantial threat for rice production ultimately risks for food security. To overcome various types of abiotic stress; seed priming is among the effective approaches to improve the rice seed germination and growth vigor. Therefore, the present study was planned to evaluate physiological and biochemical modifications in Chinese and Pakistani rice varieties by Qiangdi 863 biological assistant growth apparatus nano treated water (NTW), Osmopriming Calcium chloride (CaCl₂), redox priming hydrogen peroxide (H₂O₂) and hormonal priming by Salicylic acid (SA) under temperature stress conditions. The experiment was performed with completely randomize design conditions. Five rice varieties, nomenclature as Zhongzoa 39, (Chinese rice variety) KSK 133, KS 282, Super basmati and PK 1121 aromatic (Pakistani rice variety) were sown under low temperature (LT) (17ºC), optimal temperature (OT) 27ºC and high temperature (HT) 37ºC conditions. The present study indicated that nanopriming were the most effective treatments increased Germination Energy Percentage (GEP) (96.1, 100, 100%), Speed of Germination (SG) (27.2, 35.45, 37.1), Final Germination Percentage (FGP) (98.2, 99.1, 99.4%), Seedling Dry Weight Biomass (DWB) (0.1, 0.137, 0.14g), Total Chlorophyll Content (0.502, 13.74, 15.21), antioxidant enzymes Superoxide Dismutase (SOD)(3145, 2559, 3345 µg^-1FWh^-1), Catalase (CAT) (300, 366, 3243 µg^-1FWh^-1) and decreased Malondialdehyde (MDA) (6.5, 12.2, 6.5 µmol g^-1 FW) for Zhongzao 39 and KSK 133 rice varieties under low (LT+NTW), optimal temperature (OP+NTW) and high temperature (HT+NTW) stress., Therefore, nano-priming is recommended to cope with the high and low-temperature stress conditions along with improved productivity of rice.

Keywords: antioxidative enzymes; high temperature; low temperature; seed priming

Rice varieties	Parentage/Pedigree	Year of Release	Breeding Center	Province/Country
Zhongzao 39	Jiayu 253/Zhongzu no.3	2012	CNRRI, Hangzhou	Zhejiang, China
KSK 133	KS 282× 4321	2006	KSK, Lahore	Punjab, Pakistan
KS 282	Basmati 370×IR95	1982	KSK, Lahore	Punjab, Pakistan
Super Basmati	Basmati×10486	1996	KSK, Lahore	Punjab, Pakistan
PK 1121 aromatic	Selection from farmer’s fields for extra-long grain and high elongation ratio	2013	KSK, Lahore	Punjab, Pakistan

Rice Varieties	Treatment	GEP (%)			SG (seeds/day)			FGP (%)
Rice Varieties	Treatment	17^oC	27^oC	37^oC	17^oC	27^oC	37^oC	17^oC	27^oC	37^oC
Zhongzao 39	Control	65.0±1.8	66.0±5.7	85.0±5.9	19.7±7.4	30.2±4.4	19.0±14	75.0±7	60.0±3	90.0±5.1
	Nano	96.8±1.6*	98.4±2.8*	97.3±1.2*	27.2±1.7*	35.4±2.1*	37.1±7.7*	96.6±2.1*	97.5±2.1*	98.5±1.5*
	CaCl₂	94.6±5.7	94.0±7.5	83.3±5.7	23.6±1.9	34.1±2.5	24.6±8.7	92.0±5.7	96.2±2.8	94.1±2.8
	H₂O₂	95.1±2.8	93.0± 8.6	87.3±10.0	26.8±2.0	32.4±1.9	29.1±6.4	95.0±2.8	94.6±5.7	96.3±5.7
	SA	93.3±7.6	87.0±11.0	86.0±1.5	22.9±7.0	31.9±2.9	22.3±3.2	86.67±2.3	93.3±11	91.0±8.6
KSK 133	Control	71.7±28.0	86.7±2.8	68.3±28.0	5.7±5.6	23.2±9.2	23.1±11.0	75.0±15.0	87.3±9.0	67.0 ±3.0
	Nano	96.1±5.1*	100.0±0.0	100.0±0.0*	35.0±1.4*	36.1±0.3*	38.6±1.8*	98.2±2.8*	99.1±2.1*	99.4±2.8*
	CaCl₂	91.7±14.4	99.0±1.3*	84.2±1.1	31.2±6.1	36.1±0.7	31.2±1.0	94.4±1.0	96.2±3.2	92.7±5.7
	H₂O₂	93.3±7.6	98.3±2.8	87.0±2.8	33.4±2.9	34.4 ±0.4	33.4±1.0	96.6±5.7	94.6±5.7	96.8±6.1
	SA	80.0±10	78.3±37.0	80.0±5.9	25.9±10.0	27.7±14.0	30.04 ±1.1	87.0±10.0	40.0±15.0	83.34±10.0
KS 282	Control	43.3±27.0	93.3±11.0	53.3±40.0	16.4±3.2	20.8±4.0	19.0±14.0	86.7±23.0	90.0±10.0	88.0±11.5
	Nano	94.0±1.4*	98.3±2.8*	99.0±0.5*	33.0±0.8*	38.5±0.21*	37.2±1.0*	98.5±2.3*	98.0±1.3*	98.8±1.5*
	CaCl₂	85.0±10.0	97.2±3.0	96.3±2.8	25.3±1.0	35.8±0.8	34.7±2.3	96.0±5.7	98.7±5.7	93.3±2.8
	H₂O₂	91.7±2.8	96.6±5.7	98.7±5.7	31.6±1	33.2 ±1.7	26.4±2.8	98.0±2.8	96.3±2.8	95.0±8.6
	SA	83.3±11.0	70.0±27.0	93.0±4.3	17.8±1.7	23.5±9.2	31.6±1.6	93.0±7.6	68.3±10.0	91.3±5.7
Super Basmati	Control	50.0±32.0	73.3±10.0	80.0±17.3	16.6± 3.0	14.9±3.2	10.4±0.7	90.0±8.6	66.7±2.8	61.7±10.0
	Nano	95.0±3.2*	98.3±2.8*	98.3±0.7*	30.0±1.0*	38.1±0.7*	35.2±1.3*	98.1±2.3*	98.2±1.2*	98.88±1.5*
	CaCl₂	73.3±10.0	94.3±3.1	93.7±7.6	20.9±1.2	36.0±1.1	26.6±2.8	96.0±5.7	98.3±2.8	86.67±2.3
	H₂O₂	93.3±5.7	92.6±5.7	96.7±5.7	27.4±3.4	31.4±4.9	30.5±1.9	98.0±2.8	96.7±5.7	98.3±2.8
	SA	65.0±10.0	85.0±10.0	91.0±5.0	18.5±9.3	18.0±4.0	23.1±2.9	93.3±5.0	93.3±11.0	83.7±5.7
PK 1121 aromatic	Control	1.7±2.8	56.7±14.0	30.0±5.0	1.3±0.7	17.6±3.1	9.0±0.8	10.0±5.0	61.7±10.0	36.7±7.6
	Nano	33±1*	68.3±3.2*	69.3±6.5*	6.5±1.4^* * Values were mean standard error ± (n=3) with different treatments. Mean values are mentioned. P-Value < 0.05.	25.5±2.5*	26.5±1.1*	42.2±2.3*	77.0±1.3*	78.0±3.2*
	CaCl₂	13±4	65.4±5.7	50.0±8.6	4.9±1.9	22.2±4	16.9±1.2	28.34±20.0	73.6±2.8	63.7±5.7
	H₂O₂	21.67±1.0	63.0±5.0	52.3±7.6	6.0±4.1	21.1±3.0	20.3±1.5	38.4±2.6	66.3±14.0	71.3±7.6
	SA	10±5	31.6±15.0	35.0±5.0	3.53±0.9	9.5 ±5.7	10.7±3.0	26.7±7.6	36.67±10.0	60.7±10.0

Treatments	Germination			Chlorophyll
Treatments	DF	F-Value	P-Value	DF	F-Value	P-Value
Variety	4	6715	***	4	27.00	***
Temperature	2	117	***	2	270.00	***
Priming	4	2052	***	4	5.05	***
Variety*temperature	8	358	***	8	7.08	***
Variety*priming agent	12	273	***	12	4.47	***
Temperature*priming agent	6	456	***	6	5.46	***
Varietytemperaturepriming agent	24	227	***	24	1.53	**
Treatments	DW			CAT
	DF	F-Value	P-Value	DF	F-Value	P-Value
Variety	4	6.28	***	4	0.524	Non-sig
Temperature	1	28.8	***	2	32.70	***
Priming	4	1.508	Non-sig	4	3.04	**
Variety*temperature	4	10.46	***	7	0.40	Non-sig
Variety*priming agent	12	0.758	Non-sig	12	1.48	Non-sig
Temperature*priming agent	3	0.603	Non-sig	6	2.86	**
Varietytemperaturepriming agent	12	2.537	***	19	1.73	**
	SOD			MDA
Treatments	DF	F-Value	P-Value	DF	F-Value	P-Value
Variety	4	2.605	**	4	4.01	***
Temperature	2	7.021	Non-sig	2	7.70	***
Priming	4	5.57	**	4	14.70	***
Variety*temperature	8	1.86	Non-sig	8	10.12	***
Variety*priming agent	12	1.46	Non-sig	12	4.33	***
Temperature*priming agent	6	2.11	**	6	3.47	***
Varietytemperaturepriming agent	24	1.31	**	24	3.47	***

Rice varieties	Treatment	Chlorophyll a			Chlorophyll b			Total chlorophyll
Rice varieties	Treatment	17^oC	27^oC	37^oC	17^oC	27^oC	37^oC	17^oC	27^oC	37^oC
Zhongzao 39	Control	0.02	1.62	8.04	0.04	0.52	2.34	0.06	2.14	10.38
	Nano	0.20*	8.81*	11.41*	0.30*	4.93*	3.80*	0.50*	13.74*	15.21*
	CaCl₂	0.10	8.22	9.25	0.16	2.76	3.50	0.26	10.98	12.75
	H₂O₂	0.15	2.90	10.14	0.27	1.03	3.19	0.42	3.94	13.33
	SA	0.07	0.47	7.06	0.04	0.27	3.92	0.11	0.88	10.97
KSK 133	Control	0.04	1.07	3.25	9.15	0.42	0.99	9.19	1.49	4.24
	Nano	0.13*	8.93*	15.00*	20.10*	3.21*	4.50*	24.01*	23.86*	22.01*
	CaCl₂	0.08	8.45	8.37	17.93	3.01	2.36	23.1	11.47	10.73
	H₂O₂	0.11	6.53	14.64	15.60	2.41	4.31	23.71	8.94	18.95
	SA	0.06	3.26	7.46	9.76	1.54	2.07	10.81	4.80	9.53
KS 282	Control	0.01	1.62	8.04	0.02	0.22	2.34	0.03	1.83	10.38
	Nano	0.09*	10.32*	13.52*	0.048*	2.94*	4.53*	0.14*	13.65*	17.77*
	CaCl₂	0.04	8.22	12.24	0.04	1.04	3.24	0.08	9.26	15.48
	H₂O₂	0.08	9.90	13.15	0.04	2.73	4.30	0.13	12.6	17.45
	SA	0.02	0.47	10.05	0.03	0.54	2.87	0.05	1.01	12.92
Super Basmati	Control	0.03	0.68	2.26	0.05	0.23	0.62	0.08	0.91	2.89
	Nano	0.07*	4.42*	9.11*	0.12*	2.54*	2.88*	0.19*	7.00*	11.98*
	CaCl₂	0.06	4.21	2.44	0.06	2.33	1.15	0.12	6.54	3.59
	H₂O₂	0.06	3.56	8.86	0.07	2.24	2.53	0.13	5.79	11.39
	SA	0.04	1.13	6.46	0.059	0.79	1.80	0.10	1.92	8.27
PK 1121 aromatic	Control	0.01	0.04	0.10	0.01	0.65	1.73	0.02	0.69	1.83
	Nano	0.05*	1.95*	10.64*	0.10^* * Mean values are mentioned. P-Value < 0.05.	1.12*	5.01*	0.15*	3.10*	15.65*
	CaCl₂	0.04	1.44	10.07	0.06	1.25	4.76	0.09	2.69	14.83
	H₂O₂	0.04	1.76	6.33	0.08	1.10	3.87	0.13	2.87	10.20
	SA	0.035	1.39	4.71	0.04	0.05	2.77	0.08	1.45	7.47

vertical_align_top file_download show_chart

more_horizclose
- image
- translate
- link
- article
- vertical_align_top
- file_download
- show_chart
- image
- translate
- link
- article

location_on

Instituto Internacional de Ecologia R. Bento Carlos, 750, 13560-660 São Carlos SP - Brasil, Tel. e Fax: (55 16) 3362-5400 - São Carlos - SP - Brazil
E-mail: bjb@bjb.com.br

rss_feed Acompanhe os números deste periódico no seu leitor de RSS

Acessibilidade / Reportar erro

[TFN1] ^*
Values were mean standard error ± (n=3) with different treatments_. Mean values are mentioned. P-Value < 0.05.