Seasonality effect on the allelopathy of cerrado species

Efeito da sazonalidade sobre a alelopatia de espécies de cerrado

Abstracts

The Brazilian cerrado presents strong climate seasonality. During the dry season, plants may be exposed to stressful situations, such as a soil surface water deficit, that stimulate their chemical defenses. However, the seasonality effect on the production of allelopathic compounds of cerrado plant species is poorly understood. In this study, the phytotoxic activities of common native cerrado plants were evaluated during rainy and dry seasons. Crude leaves extracts (10% concentration: weight/volume, with dry leaves and distilled water) from eleven species were tested on lettuce and sesame germination. The negative effects on germination percentages, rates and informational entropies of the target species were higher when submitted to plant extracts from the dry season, where the germination rate was the most sensible parameter. The higher sensibility of lettuce and the germination rate parameter showed this difference. Only two exceptions had higher effects for rainy season extracts; one species showed higher negative effects on germination informational entropy of lettuce and another species on the germination rate of sesame. Thus, increases in the allelopathic activity were seen in the majority of the studied cerrado plant species during the dry season. These distinct responses to stressful situations in a complex environment such as the Brazilian cerrado may support the establishment and survival of some species.

chemical ecology; phytotoxicity; secondary metabolites

O cerrado brasileiro apresenta forte sazonalidade climática. Durante a estação seca as plantas podem estar expostas a situações estressantes, tais como um déficit de umidade nas camadas superficiais do solo, que estimulam suas defesas químicas. No entanto, o efeito da sazonalidade na produção de compostos alelopáticos de espécies vegetais de cerrado é pouco conhecido. Neste estudo, as atividades fitotóxicas de plantas nativas comuns de cerrado foram avaliadas durante as estações chuvosa e seca. Extratos brutos de folhas (concentração 10%: peso/volume, com folhas secas e água destilada) de onze espécies foram testados sobre a germinação de alface e gergelim. Os efeitos negativos sobre as porcentagens de germinação, velocidades e entropias informacionais das espécies-alvo foram maiores quando submetidas a extratos vegetais da estação seca. A maior sensibilidade da alface e do parâmetro velocidade de germinação mostraram essa diferença. Apenas duas exceções tiveram maiores efeitos para extratos da estação chuvosa; uma espécie mostrou maiores efeitos negativos sobre a entropia informacional de germinação de alface e outra espécie sobre a velocidade de germinação de gergelim. Assim, aumentos na atividade alelopática foram vistos na maioria das espécies vegetais do cerrado estudadas durante a estação seca. Estas respostas distintas a situações estressantes em um ambiente complexo como o cerrado brasileiro podem auxiliar no estabelecimento e na sobrevivência de algumas espécies.

ecologia química; fitotoxicidade; metabólitos secundários

1.

Introduction

Climate conditions can regulate plant interactions (Kikvidze et al., 2011). Precipitation regime, temperature, humidity, radiation and soil moisture can affect plant secondary metabolism (Gobbo-Neto and Lopes, 2007; Taiz and Zeiger, 2010) and may relate to plant chemical interactions.

The Brazilian cerrado, the largest savanna in South America, once covered 22% of the country's land surface (22 million ha), primarily in its Central Plateau. It has a seasonal climate, with a dry season from April to September and a wet season from October to March (Gottsberger and Silberbauer-Gottsberger, 2006). During the former, the soil presents a surface water deficit (Haridasan, 2001) that may result in a stressful situation for plants in this environment (Sarmiento, 1996).

Abiotic and biotic stress conditions seemingly improve plant allelopathic activity (Gross, 2003). Allelopathy is defined by the International Allelopathy Society (IAS, 2013) as any process involving secondary metabolites produced by plants, algae, bacteria and fungi that influences the growth and development of agricultural and biological systems. Allelopathy is strongly related to plant-environment interactions (Blanco, 2007). Allelochemicals can benefit or prejudice certain species, influencing the qualitative and quantitative floristic composition in time and space (Durigan and Almeida, 1993). Furthermore, allelopathy may influence forest succession process (Peng et al., 2004) and spatial patterns of species distribution (Inderjit and Callaway, 2003). Allelopathic influence can be expressed on seed germination and/or establishment and development of neighbouring individuals (Ferreira, 2005).

Although seasonal allelopathic variation has been reported, few studies consider this issue for native cerrado species (Oliveira, 2009; Orlandi et al., 2010). It is necessary to examine seasonal allelopathic variation from several species. In this study, the allelopathic potential of eleven cerrado species from nine different families and two different collection periods were assessed to determine the existence and extent of seasonal variation.

2.

Material and Methods

2.1.

Collection, study area and climate

Mature plant leaves of eleven cerrado species were collected in a cerrado sensu stricto area of the Universidade Federal de São Carlos (21°8′5″S and 47°53′12″W), São Carlos, São Paulo, Brazil, on 30/03/2006 and 27/07/2006. This area is classified as Cwa (according to Köeppen (1948)) with warm climate and dry winter. The average monthly pluviometry and temperature were 207 mm and 23°C during the rainy season (October 2005 - March 2006) and 18 mm and 20°C during the dry season (April - September 2006) (Embrapa, 2012).

The species studied were Anadenanthera falcata(Benth.) Speg. (Fabaceae), Davilla elliptica A.St.-Hil. (Dilleniaceae), Diospyros hispida A.DC. (Ebenaceae), Kielmeyera coriacea Mart. & Zucc. (Clusiaceae), Miconia albicans (Sw.) Steud. (Melastomataceae), Piptocarpha rotundifolia Baker (Asteraceae), Schefflera vinosa (Cham. & Schltdl.) Frodin & Fiaschi (Araliaceae), Senna rugosa(G.Don) H.S.Irwin & Barneby (Fabaceae), Siparuna guianensis Aubl. (Monimiaceae), Stryphnodendron polyphyllum Mart. (Fabaceae), and Xylopia aromatica Mart. (Annonaceae) (Ipni, 2012). These species present different habits, shrub or tree (Table 1), and the collection of the leaves was unsystematic.

Table 1.
Osmotic potential values (MPa) of the cerrado species crude leaves extracts (10% concentration: weight/volume, with dry leaves and distilled water) from rainy and dry seasons.

Species	Habit	Osmotic potential (MPa)
		Rainy season	Dry season
Anadenanthera falcata(Benth.) Speg.	Tree	−0.141	−0.092
Davilla ellipticaA.St.-Hil.	Shrub	−0.181	−0.132
Diospyros hispida A.DC.	Shrub or tree	−0.104	−0.100
Kielmeyera coriacea Mart. & Zucc.	Shrub or tree	−0.195	−0.201
Miconia albicans(Sw.) Steud.	Shrub or tree	−0.128	−0.050
Piptocarpha rotundifoliaBaker	Shrub	−0.129	−0.020
Schefflera vinosa(Cham. & Schltdl.) Frodin & Fiaschi	Shrub or tree	−0.183	−0.065
Senna rugosa(G.Don) H.S.Irwin & Barneby	Shrub	−0.138	−0.110
Siparuna guianensisAubl.	Shrub or tree	−0.207	−0.192
Stryphnodendron polyphyllumMart.	Tree	−0.128	−0.080
Xylopia aromatica Mart.	Shrub or tree	−0.138	−0.115

2.2.

Extract preparation

The leaves were dried at 55°C for 72 hours and stored in plastic bags at ambient temperature until use. Crude extracts (10% concentration: weight/volume) with dry leaves and distilled water were prepared and ground with an industrial blender for approximately one minute. The extracts were stored at an ambient temperature for three hours, filtered using a vacuum pump coupled to a Büchner funnel covered with filter paper (3 µm), and tested on the germination of lettuce (Lactuca sativa L.) and sesame (Sesamum indicumL.).

2.3.

Germination bioassay

Thirty cypselas/seeds of the target species (lettuce/sesame) were placed in Petri dishes (9 cm diameter) containing a double layer of filter paper (3 µm) moistened with 5 mL of extract or distilled water (witness). All Petri dishes were sealed with PVC film. The lids were closed, and the Petri dishes were placed in a BOD incubator (light-dark cycle of 12 h-12 h, 20±1°C for lettuce, and 28±2°C for sesame). The germinated cypselas/seeds were counted at 12 h intervals for one week and at 24 h intervals until the tenth day of sowing. Seeds with 2 mm radicular protrusion were considered to be germinated and removed to avoid recounting. At the end of the tenth day, the germination percentage, rate and informational entropy were calculated (Santana and Ranal, 2004) as follows:

1. G(%) = (N/A).100, where: G = germination percentage; N = number of germinated seeds; and A = total number of sowed seeds.

2. R = 1/t, where: R = the germination rate (days^–1); t = average time of germination (t = Σ(n_i.t_i)/Σn_i, where: t_i = time of incubation, and n_i = number of seeds germinated between two consecutive times of observation, (t_i - 1) and (t_i)).

3. E = −Σ[f_i.log₂(f_i)], where E = informational entropy of germination, f_i= relative germination frequency, and f_i = n_i/Σn_i, n_i = number of seeds germinated between two consecutive times of observation, (t_i - 1) and (t_i).

2.4.

Osmotic potential

The osmotic potentials of the extracts were measured (Micro-Osmette, model: 5004 automatic osmometer). A germination bioassay was set with the target species subjected to treatments of polyethylene glycol 6000 in concentrations of corresponding obtained osmotic potentials.

2.5.

Data analysis

The experimental design was completely randomised with four repetitions. The data were compared by the Mann-Whitney test (α=0.05), using PAST version 2.5 software (Hammer et al., 2001).

3.

Results

The plant extracts presented osmotic potential values between -0.020 and -0.207 MPa (Table 1). The tested PEG-6000 solutions (−0.1 and −0.2 MPa) had no influence on lettuce and sesame germination percentages, rates and informational entropies.

Lettuce experiment results are shown in Table 2. Germinability reductions in relation to the witness were stronger under the effects of D. elliptica and M. albicans extracts from the dry season. All species extracts from both seasons (rainy and dry) delayed the germination in relation to the witness. However, the germination rate was lower for dry season extracts of six species (D. elliptica, D. hispida, K. coriacea, M. albicans, P. rotundifolia and S. vinosa). Germination entropy was higher for dry season extracts of four species (D. hispida, K. coriacea, P. rotundifolia and S. vinosa). D. elliptica was the only species that presented rainy season extract with higher negative effects on entropy than the dry season extract.

Table 2.
Effects on lettuce (Lactuca sativa L.) germination by leaf crude extracts (10% concentration: weight/volume, with dry leaves and distilled water) from cerrado species (Anadenanthera falcata(Benth.) Speg., Davilla elliptica A.St.-Hil., Diospyros hispida A.DC., Kielmeyera coriacea Mart. & Zucc., Miconia albicans (Sw.) Steud., Piptocarpha rotundifolia Baker, Schefflera vinosa(Cham. & Schltdl.) Frodin & Fiaschi, Senna rugosa (G.Don) H.S.Irwin & Barneby, Siparuna guianensis Aubl., Stryphnodendron polyphyllum Mart., and Xylopia aromaticaMart.), after 10 days of bioassay.

Species	G(%)		R(days–1)		E
	Rainy	Dry	Rainy	Dry	Rainy	Dry
A. falcata	96.7±2.7	95.8±5.0	0.50±0.09	0.46±0.02	1.83±0.33	1.94±0.15
D. elliptica	97.5±1.6	24.2±19.7*	0.17±0.01	0.11±0.00*	2.83±0.31*	1.60±0.56
D. hispida	93.3±5.4	83.3±8.2	0.48±0.07	0.15±0.02*	1.68±0.45	3.10±0.15*
K. coriacea	93.3±6.1	90.8±7.4	0.46±0.04	0.32±0.07*	1.79±0.37	2.62±0.30*
M. albicans	88.3±4.3	36.7±16.8*	0.28±0.03	0.13±0.01*	3.21±0.25	1.90±0.87
P. rotundifolia	93.3±6.1	91.7±4.3	0.50±0.03	0.27±0.06*	1.71±0.22	2.71±0.32*
S. vinosa	90.8±5.7	94.2±7.4	0.42±0.05	0.17±0.05*	2.29±0.24	2.97±0.08*
S. rugosa	90.8±3.2	95.0±1.9	0.49±0.02	0.44±0.05	1.86±0.17	1.99±0.23
S. guianensis	92.5±3.2	95.0±6.4	0.22±0.02	0.20±0.02	2.91±0.09	2.84±0.34
S. polyphyllum	95.8±4.2	93.3±7.7	0.48±0.05	0.54±0.02	1.72±0.30	1.87±0.17
X. aromatica	93.3±6.1	93.3±7.2	0.30±0.03	0.30±0.04	2.33±0.23	2.51±0.56
Witness	99.2±1.7	97.5±3.2	0.74±0.13	0.72±0.14	1.26±0.49	1.21±0.25

• Mean±standard deviation. G(%): germinability; R: Germination rate; E: informational entropy of germination; Rainy: rainy season; Dry: dry season. Values±standard deviation. Difference from witness (italic bold numbers) and between rainy/dry seasons (*) by Mann-Whitney test (α=0.05).

Sesame experiment results are shown in Table 3. No differences in germinability were observed in any treatments. The germination rate was decreased in 10 out of 11 species extracts. Three species (D. elliptica, P. rotundifoliaand S. vinosa) presented higher inhibitions when collected in the dry season, compared with just one species (A. falcata) in the rainy season. Few differences in the germination entropy for this target species were observed. Four species increased entropy values, but only two species collected in the dry season had higher effects.

Table 3.
Effects on sesame (Sesamum indicum L.) germination by leaf crude extracts (10% concentration: weight/volume, with dry leaves and distilled water) from cerrado species (Anadenanthera falcata(Benth.) Speg., Davilla elliptica A.St.-Hil., Diospyros hispida A.DC., Kielmeyera coriacea Mart. & Zucc., Miconia albicans (Sw.) Steud., Piptocarpha rotundifolia Baker, Schefflera vinosa(Cham. & Schltdl.) Frodin & Fiaschi, Senna rugosa (G.Don) H.S.Irwin & Barneby, Siparuna guianensis Aubl., Stryphnodendron polyphyllum Mart., and Xylopia aromaticaMart.), after 10 days of bioassay.

Species	G(%)		R(days–1)		Entropy
	Rainy	Dry	Rainy	Dry	Rainy	Dry
A. falcata	98.3±3.3	100.0±0.0	0.55±0.03*	0.65±0.05	1.40±0.35	1.10±0.51
D. elliptica	94.2±4.2	96.7±4.7	0.54±0.06	0.39±0.05*	1.42±0.43	2.14±0.28
D. hispida	100.0±0.0	100.0±0.0	0.68±0.03	0.63±0.05	0.74±0.41	1.31±0.25
K. coriacea	97.5±3.2	99.2±1.7	0.66±0.00	0.62±0.12	0.20±0.28	1.10±0.27
M. albicans	96.7±3.8	98.3±1.9	0.65±0.02	0.61±0.13	0.85±0.09	1.55±0.20*
P. rotundifolia	99.2±1.7	97.5±3.2	0.76±0.03	0.60±0.06*	0.95±0.15	1.06±0.43
S. vinosa	98.3±1.9	100.0±0.0	0.64±0.05	0.42±0.07*	0.76±0.70	2.27±0.42*
S. rugosa	98.3±3.3	98.3±1.9	0.67±0.02	0.69±0.03	0.35±0.09	0.80±0.23
S. guianensis	95.8±4.2	98.3±1.9	0.31±0.04	0.32±0.06	2.61±0.29	2.51±0.21
S. polyphyllum	98.3±3.3	100.0±0.0	0.64±0.02	0.67±0.04	0.35±0.17	0.85±0.24
X. aromatica	95.0±1.9	100.0±0.0	0.58±0.09	0.60±0.07	1.33±0.71	1.19±0.27
Witness	95.0±3.3	100.0±0.0	0.84±0.15	0.82±0.02	0.83±0.62	1.08±0.13

• Mean±standard deviation. G(%): germinability; R: Germination rate; E: informational entropy of germination; Rainy: rainy season; Dry: dry season. Values±standard deviation. Difference from witness (italic bold numbers) and between rainy/dry seasons (*) by Mann-Whitney test (α=0.05).

4.

Discussion

The osmotic potential of an extract can present a negative effect on germination (Ferreira and Aquila, 2000). Some solutes alter water osmotic potential and uptake by the plant (Villela et al., 1991). According to the osmolarity control experiment, the studied cerrado species extracts did not show osmotic potential values (from −0.020 to −0.207 MPa) that could influence lettuce and sesame germination processes.

The germination of lettuce and sesame were more impacted by dry season extracts. Decreases in the percentages and rates were more evident in target species subjected to dry season extracts. Stronger inhibitions occurred on the germination rate rather than the percentage. Higher germination entropy values and imbibition time distributions were verified, characterising lower germination synchrony. In addition to germinability, germination rate, homogeneity and synchrony are factors that show the level of organisation or disorder in the chemical reactions of the germination process (Ferreira, 2004; Santana et al., 2006). Changes in germination parameters can result from the effects of several physiological processes: membrane permeability, DNA transcription and translation, second messengers function, respiration, enzymes and receptors conformation (Ferreira, 2004; Rizvi and Rizvi, 1992). Slower germination plants can present reduced size (Jefferson and Pennacchio, 2003), leading to greater susceptibility to stress and less chances in resource competition. The species' sensibility to phytotoxins under laboratory conditions is dependent of biochemical/physiological characteristics (Kobayashi, 2004), seed size and structure.

Because phytotoxic effects on the germination processes can be species dependent (Oliveira, 2009), two target species (lettuce and sesame) were selected. Dependency on both donor and receptor species was observed. Donor species differed in their effects; some species presented inhibitions on none, some or all of the germination parameters. The receptor species sesame was less sensible compared to lettuce but presented similar results of higher negative effect when subjected to dry season extracts. Lettuce has been considered to be too sensitive for allelopathic bioassays, and this study detected more differences in phytotoxic activities between the extracts from the rainy and dry season collection periods.

Cerrado presents a dry season that lasts up to six months (Gottsberger and Silberbauer-Gottsberger, 2006). The soil is subjected to a water deficit at the surface layers due to increasing atmospheric evaporation demand and solar incidence (Franco, 2002; Haridasan, 2001) that represents a potential stressful time for plants (Sarmiento, 1996). Biotic and abiotic stressful conditions alter the production of allelochemicals. Climatic parameters can affect the production of flavonoids (Chaves and Escudero, 1999) and increase cumarine concentration in some plants (Blanco, 2007). Flavonoids are found in nearly all vascular plants, and some flavonoids can affect germination (Iwashina, 2003). In addition, plants are rarely exposed to only one stress (Einhellig, 1999). Other factors, which possibly interact in this environment, include competition processes, microorganism activity, vegetation burning and leaves deciduousness influenced by seasonality.

Environments in stressful conditions may restrict plant growth, photosynthetic ratio and accumulate nonstructural carbohydrates. This may be one of the reasons contributing to increased production of carbon based defensive substances, which are secondary metabolism derivatives (Chaves and Escudero, 1999). The carbon/nutrient balance is confirmed because species growing in low nutrient/water availability environments produce high levels of tannins and phenols (Bryant et al., 1983; Waring et al., 1985).

Allelopathy can present an important function in arid environments. Allelochemicals in arid environments remain in the soil longer compared with humid environments, and their effects can be intensified when combined with the impact of low soil moisture (Einhellig, 1999). The production of these compounds can increase in environments where burning is common, most likely due to physical damage to plants. Increased production can provide a higher inhibition on competitor/receptor plants and characterise an important plant defense mechanism (Einhellig, 1999; Einhellig, 2004).

The difference observed in the results can be due to species exposure to climate alterations or other seasonal stresses that induced greater allelochemical production. The allelopathic activity on both receptor species increased during the dry season for the majority of the studied cerrado plant species. Lettuce was the most sensible receptor species, and the germination rate was the most sensible parameter that showed this difference. These distinct responses to stressful situations in a complex environment, such as the Brazilian cerrado, may support species establishment and survival. Therefore, studies of other species are necessary to better understand this topic.

Acknowledgements

We are grateful to CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) for supporting the research for the first author and for funding the Research Productivity Grant for the last author.

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Publication Dates

• Publication in this collection
  Aug 2014

History

• Received
  2 Oct 2012
• Accepted
  17 June 2013
• Reviewed
  30 Nov 2014