Perfil molecular da resistência à ferrugem bacteriana em cultivares de arroz da Malásia

Javed, M. A.; Ali, S. W.; Ashfaq, M.; Tabassam, J.; Ali, M.; IhsanUllah, M.; Nayab, S. F.; Kaya, Y.; Khalili, E.; Ali, Q.; Yau, T. E.

M. A. Javed ^**Corresponding author: majaved.iags@pu.edu.pk

University of the Punjab, Faculty of Agricultural Sciences, Department of Plant Breeding and Genetics, Lahore, Pakistan

Universiti Teknologi Malaysia - UTM Skudai, Faculty of Science, Department of Biosciences, Johor Bahru, Malaysia

https://orcid.org/0000-0001-9629-4215

S. W. Ali

University of the Punjab, Department of Food Science and Technology, Faculty of Agricultural Sciences, Lahore, Pakistan

M. Ashfaq

University of the Punjab, Faculty of Agricultural Sciences, Department of Plant Breeding and Genetics, Lahore, Pakistan

J. Tabassam

University of the Punjab, Faculty of Agricultural Sciences, Department of Plant Breeding and Genetics, Lahore, Pakistan

M. Ali

University of the Punjab, Faculty of Agricultural Sciences, Department of Entomology, Lahore, Pakistan

M. IhsanUllah

Cotton Research Institute Multan, Multan, Pakistan

S. F. Nayab

Sorghum Research Sub Station, Dera Ghazi Khan, Pakistan

Y. Kaya

Ondokuz Mayis University, Faculty of Agriculture, Department of Agricultural Biotechnology, Samsun, Turkey

E. Khalili

Tarbiat Modarres University, Faculty of Science, Department of Plant Science, Tehran, Iran

Q. Ali

University of the Punjab, Faculty of Agricultural Sciences, Department of Plant Breeding and Genetics, Lahore, Pakistan

https://orcid.org/0000-0002-3160-4830

T. E. Yau

Universiti Teknologi Malaysia - UTM Skudai, Faculty of Science, Department of Biosciences, Johor Bahru, Malaysia

*Corresponding author: majaved.iags@pu.edu.pk

No.	Rice variety	Accession No.	Year of release	No.	Rice variety	Accession No.	Year of release
1	Seberang	04559	1985	20	Pulut Hitam 9	08363	1991
2	Ria	01478	1973	21	Mahsuri	00826	1966
3	MR220	11634	2003	22	Bahagia	00167	1964
4	MR81	04630	1986	23	MR84	04633	1988
5	Manik	04556	1984	24	Sri Malaysia II	02672	1974
6	MR232	12047	2006	25	MR263	-	2010
7	Murni	01041	1972	26	Sekembang	04553	1979
8	MR123	07488	1990	27	Maswangi (MRQ74)	11787	2005
9	MR159	08638	1995	28	MR219	11633	2001
10	MR106	07487	1990	29	Jaya (Malaysia)	00581	1965
11	MR167	08646	1997	30	Pulut Siding	04555	1981
12	Sri Malaysia I	02125	1974	31	MR127	07489	1991
13	MRM 16 (Padi Merah)	-	2010	32	Kadaria	04554	1981
14	Muda	04557	1984	33	Malinja	00839	1968
15	Sekencang	04552	1979	34	MR253	12095	2010
16	Setanjung	04551	1979	35	Masria	00860	1972
17	MR185	08455	1995	36	Puteri (MRQ50)	09345	1999
18	Makmur	04558	1984	37	MR103	07486	1990
19	Pulut Malaysia I	02123	1974	38	MR211	1629	1999

No.	Marker (Gene)	Location ^*	Primer sequence	References
1	RM122 (xa5)	5	F: GAGTCGATGTAATGTCATCAGTGC	Shanti et al. (2010)SHANTI, M.L., SHENOY, V.V., LALITHA, D.G., MOHAN, K.V., PREMALATHA, P., NAVEEN, K.G., SHASHIDHAR, H.E., ZEHR, U.B. and FREEMAN, W.H., 2010. Marker-assisted breeding for resistance to bacterial leaf blight in popular cultivar and parental lines of hybrid rice. Journal of Plant Pathology, vol. 1, no. 3, pp. 495-501. http://dx.doi.org/10.4454/jpp.v92i2.194. http://dx.doi.org/10.4454/jpp.v92i2.194...
1	RM122 (xa5)	5	R: GAAGGAGGTATCGCTTTGTTGGAC
2	RM21 (xa5)	11	F:ACAGTATTCCGTAGGCACGG	Pha and Lan (2004)PHA, N.T. and LAN, N.T., 2004. Marker assisted selection in rice breeding for bacterial leaf blight. Omon Rice, vol. 12, pp. 19-26.
2	RM21 (xa5)	11	R:GCTCCATGAGGGTGGTAGAG
3	RM206 (xa10)	11	F:CCCATGCGTTTAACTATTCT	Chen et al. (1997)CHEN, S., TEMNYKH, S., XU, Y., CHO, Y.G. and MCCOUCH, S.R., 1997. Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.). Theoretical and Applied Genetics, vol. 95, no. 4, pp. 553-567. http://dx.doi.org/10.1007/s001220050596. http://dx.doi.org/10.1007/s001220050596...
3	RM206 (xa10)	11	R:CGTTCCATCGATCCGTATGG
4	RM159 (xa5)	5	F:GGGGCACTGGCAAGGGTGAAGG	Pinta et al. (2013)PINTA, W., TOOJINDA, T., THUMMABENJAPONE, P. and SANITCHON, J., 2013. Pyramiding of blast and bacterial leaf blight resistance genes into rice cultivar RD6 using marker assisted selection. African Journal of Biotechnology, vol. 12, no. 28, pp. 4432-4438. http://dx.doi.org/10.5897/AJB12.2028. http://dx.doi.org/10.5897/AJB12.2028...
4	RM159 (xa5)	5	R:GCTTGTGCTTCTCTCTCTCTCTCTCTCTC
5	xa13 promoter (xa13)	8	F:TCCCAGAAAGCTACTACAGC	Hajira et al. (2016)HAJIRA, S.K., SUNDARAM, R.M., LAHA, G.S., YUGANDER, A., BALACHANDRAN, S.M., VIRAKTAMATH, B.C., SUJATHA, K., BALACHIRANJEEVI, C.H., PRANATHI, K., ANILA, M., BHASKAR, S., ABHILASH, V., MAHADEVASWAMY, H.K., KOUSIK, M., KUMAR, T.D., HARIKA, G. and REKHA, G., 2016. A single-tube, functional markerbased multiplex PCR assay for simultaneous detection of major bacterial blight resistance genes xa21, xa13 and xa5 in Rice. Rice Science, vol. 23, no. 3, pp. 144-151. http://dx.doi.org/10.1016/j.rsci.2015.11.004. http://dx.doi.org/10.1016/j.rsci.2015.11...
5	xa13 promoter (xa13)	8	R:GCAGACTCCAGTTTGACTTC
6	RM224 (xa4)	11	F: ATCGATCGATCTTCACGAGG	Chen et al. (1997)CHEN, S., TEMNYKH, S., XU, Y., CHO, Y.G. and MCCOUCH, S.R., 1997. Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.). Theoretical and Applied Genetics, vol. 95, no. 4, pp. 553-567. http://dx.doi.org/10.1007/s001220050596. http://dx.doi.org/10.1007/s001220050596...
6	RM224 (xa4)	11	R: TGCTATAAAAGGCATTCGGG
7	pTA 248 (xa21)	11	F:AGACGCGGGAAGGGTGGTTCCCGGA	Davierwala et al. (2001)DAVIERWALA, A.P., REDDY, A.P.K., LAGU, M.D., RANJEKAR, P.K. and GUPTA, V.S., 2001. Marker assisted selection of bacterial blight resistance genes in rice. Biochemical Genetics, vol. 39, no. 7-8, pp. 261-278. http://dx.doi.org/10.1023/A:1010282732444. PMid:11590832. http://dx.doi.org/10.1023/A:101028273244...
7	pTA 248 (xa21)	11	R:AGACGCGGGTAATCGAAAGATGAAA
8	RM251 (xa7/8)	3	F: GAATGGCAATGGCGCTAG	Chen et al. (1997)CHEN, S., TEMNYKH, S., XU, Y., CHO, Y.G. and MCCOUCH, S.R., 1997. Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.). Theoretical and Applied Genetics, vol. 95, no. 4, pp. 553-567. http://dx.doi.org/10.1007/s001220050596. http://dx.doi.org/10.1007/s001220050596...
8	RM251 (xa7/8)	3	R: ATGCGGTTCAAGATTCGATC
9	RM317 (xa2)	4	F:CATACTTACCAGTTCACCGCC	He et al. (2006)HE, Q., LI, D.B., ZHU, Y.S., TAN, M.P., ZHANG, D.P. and LIN, X.H., 2006. Fine mapping of Xa2, a bacterial blight resistance gene in rice. Molecular Breeding, vol. 17, no. 1, pp. 1-6. http://dx.doi.org/10.1007/s11032-005-8698-2. http://dx.doi.org/10.1007/s11032-005-869...
9	RM317 (xa2)	4	R:CTGGAGAGTGTCAGCTAGTTGA
10	RM13 (xa5)	5	F:TCCAACATGGCAAGAGAGAG	McCouch et al. (1988)MCCOUCH, S.R., KOCHERT, G., YU, Z.H., WANG, Z.Y., KHUSH, G.S., COFFMAN, W.R. and TANKSLEY, S.D., 1988. Molecular mapping of rice chromosomes. Theoretical and Applied Genetics, vol. 76, no. 6, pp. 815-829. http://dx.doi.org/10.1007/BF00273666. PMid:24232389. http://dx.doi.org/10.1007/BF00273666...
10	RM13 (xa5)	5	R:GGTGGCATTCGATTCCAG
11	RM167 (xa3/4)	11	F:GATCCAGCGTGAGGAACACGT	Wu and Tanksley (1993)WU, K.S. and TANKSLEY, S.D., 1993. Abundance, polymorphism and genetic mapping of microsatellites in rice. Molecular & General Genetics : MGG, vol. 241, no. 1-2, pp. 225-235. http://dx.doi.org/10.1007/BF00280220. PMid:7901751. http://dx.doi.org/10.1007/BF00280220...
11	RM167 (xa3/4)	11	R:AGTCCGACCACAAGGTGCGTTGTC
12	RM230 (xa13)	8	F:GCCAGACCGTGGATGTTC	Chen et al. (1997)CHEN, S., TEMNYKH, S., XU, Y., CHO, Y.G. and MCCOUCH, S.R., 1997. Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.). Theoretical and Applied Genetics, vol. 95, no. 4, pp. 553-567. http://dx.doi.org/10.1007/s001220050596. http://dx.doi.org/10.1007/s001220050596...
12	RM230 (xa13)	8	R:CACCGCAGTCACTTTTCAAG
13	RM347 (xa11)	3	F: CACCTCAAACTTTTAACCGCAC	Goto et al. (2009)GOTO, T., MATSUMOTO, T., FURUYA, N., TSUCHIYA, K. and YOSHIMURA, A., 2009. Mapping of bacterial blight resistance gene xa11 on rice chromosome 3. Japan Agricultural Research Quarterly, vol. 43, no. 3, pp. 221-225. http://dx.doi.org/10.6090/jarq.43.221. http://dx.doi.org/10.6090/jarq.43.221...
13	RM347 (xa11)	3	R: TCCGGCAAGGGATACGGCGG
14	RM263 (xa8)	2	F: CCCAGGCTAGCTCATGAACC	Chen et al. (1997)CHEN, S., TEMNYKH, S., XU, Y., CHO, Y.G. and MCCOUCH, S.R., 1997. Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.). Theoretical and Applied Genetics, vol. 95, no. 4, pp. 553-567. http://dx.doi.org/10.1007/s001220050596. http://dx.doi.org/10.1007/s001220050596...
14	RM263 (xa8)	2	R:GCTACGTTTGAGCTACCACG

Rice cultivars		Scoring of bands in respective molecular markers (-: Absent +: Present)
		(1-14 markers as given in Table 2 )
		1	2	3	4	5	6	7	8	9	10	11	12	13	14	^# genes
1	Seberang	-	-	-	-	-	+	+	-	+	-	-	-	+	-	4
2	Ria	-	-	-	-	-	-	-	-	+	-	-	-	+	-	2
3	MR220	-	-	+	-	-	+	+	-	+	-	-	-	-	-	4
4	MR81	-	-	-	-	-	+	-	-	-	-	-	-	-	-	1
5	Manik	+	-	+	+	-	-	+	-	-	+	-	-	-	+	6
6	MR232	-	+	-	-	-	-	+	+	-	-	+	-	-	+	5
7	Murni	+	-	-	+	-	+	-	-	+	+	-	-	-	+	6
8	MR123	-	-	+	+	-	+	-	-	+	-	+	-	+	+	7
9	MR159	-	-	-	+	-	+	+	-	+	-	-	-	+	-	5
10	MR106	+	+	+	-	-	-	+	+	+	+	+	-	-	-	8
11	MR167	-	-	-	+	-	+	+	+	+	-	-	-	-	-	5
12	Sri Malaysia I	+	+	+	-	-	-	+	+	+	+	+	-	-	-	8
13	MRM16 (Padi Merah)	-	-	-	-	-	+	-	+	+	-	-	-	+	+	5
14	Muda	-	-	-	-	-	+	+	-	+	-	-	-	+	+	5
15	Sekencang	-	-	+	-	-	+	-	-	+	-	-	-	-	-	3
16	Setanjung	-	+	+	+	-	+	+	-	+	-	-	-	+	-	7
17	MR185	+	-	+	-	-	-	+	-	+	+	-	-	-	+	7
18	Makmur	-	-	-	+	-	+	+	+	+	-	-	-	+	-	5
19	Pulut Malaysia I	-	-	+	+	-	-	-	+	+	-	-	-	-	+	5
20	Pulut Hitam 9	+	-	+	-	-	+	+	+	+	+	-	-	-	+	8
21	Mahsuri	+	+	-	+	-	-	-	-	-	+	-	-	+	+	6
22	Bahagia	-	+	-	-	-	+	-	+	-	-	+	-	+	+	6
23	MR84	-	-	-	-	-	-	-	-	-	-	-	-	-	-	0
24	Sri Malaysia II	-	-	+	-	-	-	+	-	-	-	-	-	-	-	2
25	MR263	-	-	-	-	-	-	-	-	-	-	-	-	-	-	0
26	Sekembang	-	+	-	-	-	+	-	+	+	-	+	-	-	-	5
27	Maswangi (MRQ74)	+	+	+	+	-	+	+	-	+	+	-	-	+	+	10
28	MR219	+	+	-	+	-	+	+	-	+	+	-	-	-	+	8
29	Jaya (Malaysia)	+	-	+	+	-	+	-	+	+	+	-	-	+	+	9
30	Pulut Siding	-	-	-	-	-	+	-	+	+	-	+	-	-	+	5
31	MR127	-	-	-	-	-	-	+	-	-	-	-	-	+	-	2
32	Kadaria	-	-	-	-	-	-	-	-	-	-	+	-	+	-	2
33	Malinja	-	-	-	-	-	-	+	-	-	-	+	-	-	-	2
34	MR253	-	-	+	-	-	-	+	-	-	-	+	-	-	+	4
35	Masria	-	-	+	-	-	+	-	-	+	-	-	-	-	-	3
36	Puteri (MRQ50)	-	-	-	-	-	+	-	-	+	-	-	-	-	-	2
37	MR103	-	-	+	-	-	-	-	-	+	-	-	-	-	+	3
38	MR211	-	-	+	-	-	-	+	-	-	-	-	-	-	+	3
Numbers of cultivars		10	9	17	12	0	21	20	12	25	10	10	0	14	18

[1] *Corresponding author: majaved.iags@pu.edu.pk

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Perfil molecular da resistência à ferrugem bacteriana em cultivares de arroz da Malásia

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