EFFECTS OF CULTIVATION MEDIA COMPONENTS ON BIOSURFACTANT AND PIGMENT PRODUCTION FROM <i>Pseudomonas aeruginosa</i> PAO1

Das, Subhasish; Das, Palashpriya

doi:10.1590/0104-6632.20150322s00003262

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Brazilian Journal of Chemical Engineering

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Sumário

BIOPROCESS ENGINEERING • Braz. J. Chem. Eng. 32 (2) • Apr-Jun 2015 • https://doi.org/10.1590/0104-6632.20150322s00003262 copy

EFFECTS OF CULTIVATION MEDIA COMPONENTS ON BIOSURFACTANT AND PIGMENT PRODUCTION FROM Pseudomonas aeruginosa PAO1

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Biosurfactant and pigment production by P. aeruginosa have been known to be associated with its selfish growth and colonization. However, according to recent studies these products can be exploited for human benefits. In the present work the effects of culture media components on yield of these two products from P. aeruginosa PAO1 have been studied with statistical screening design experiments. Biosurfactant yield was found to be increased by two times in a modified medium. This study will help in further modifying the media composition for cheaper media development, kinetic modelling and fermentation strategy development.

Keywords
Pseudomonas aeruginosa PAO1; Biosurfactant; Pigment; Culture medium; Screening design

Expt. No.	NH₄Cl (M)	KCl (M)	Tris-HCl (M)	MgSO₄ (M)	Glycerol (%)	Peptone (%)
1	0.12	0	0	0	0	0
2	0	0.12	0	0	0	0
3	0	0	0.72	0	0	0
4	0	0	0	0.0096	0	0
5	0	0	0	0	6	0
6	0	0	0	0	0	6
7	0.07	0.01	0.06	0.0008	0.50	0.50
8	0.01	0.07	0.06	0.0008	0.50	0.50
9	0.01	0.01	0.42	0.0008	0.50	0.50
10	0.01	0.01	0.06	0.0056	0.50	0.50
11	0.01	0.01	0.06	0.0008	3.50	0.50
12	0.01	0.01	0.06	0.0008	0.50	3.50
13	0	0.024	0.144	0.002	1.20	1.20
14	0.0024	0	0.144	0.002	1.20	1.20
15	0.0024	0.024	0	0.002	1.20	1.20
16	0.0024	0.024	0.144	0	1.20	1.20
17	0.0024	0.024	0.144	0.002	0.00	1.20
18	0.0024	0.024	0.144	0.002	1.20	0.00
19	0.02	0.02	0.12	0.0016	1	1
20	0.02	0.02	0.12	0.0016	1	1
21	0.02	0.02	0.12	0.0016	1	1
22	0.02	0.02	0.12	0.0016	1	1
23	0.02	0.02	0.12	0.0016	1	1

Expt. No.	Biomass	O.D.	Biosurfactant	Pigment	Petrol	Diesel	Hexadecane
	(g/L)	(600 nm)	concentration	concentration	emulsification	emulsification	emulsification
			(g/L)	(μg/ml)	(E24)	(E24)	(E24)
1	0	0	0	0	0	0	0
2	0	0	0	0	0	0	0
3	0	0	0	0	0	0	0
4	0	0	0	0	0	0	0
5	0.18	0.08	0.1	0	0	0	0
6	2.25	0.98	0.7	6.62	0.5	0.54	0.54
7	3.36	1.46	1.1	7.84	0.5	0.52	0.55
8	3.63	1.58	0.45	2.71	0.5	0.47	0.47
9	0.87	0.38	0.5	2.78	0.31	0.33	0.41
10	3.31	1.44	0.35	4.51	0.47	0.47	0.47
11	2.25	0.98	0.5	0.72	0	0	0
12	1.33	0.58	2.4	7.49	0.6	0.5	0.52
13	3.73	1.62	0.55	11.35	0.6	0.52	0.47
14	3.08	1.34	0.7	17.25	0.5	0.52	0.47
15	2.12	0.92	0.7	9.49	0.47	0.5	0.52
16	2.67	1.16	0.25	1.88	0.25	0.4	0.35
17	1.52	0.66	0.65	0.92	0	0	0
18	1.24	0.54	0.8	0	0	0	0
19	2.53	1.1	0.85	25.69	0.6	0.3	0.42
20	2.94	1.28	1.35	15.36	0.5	0.52	0.47
21	3.27	1.42	0.88	21.34	0.5	0.48	0.45
22	2.85	1.24	1.2	18.25	0.54	0.5	0.45
23	2.53	1.1	0.98	22.53	0.6	0.5	0.5

	Source	Sequential p-value	Lack of Fit p-value	Adjusted R-Squared	Predicted R-Squared
Biomass (OD600)	Mean	< 0.0001				Suggested
	Linear	0.91	0.003	-0.19	-4.21
	Quadratic	0.05	0.007	0.33	-8.14	Aliased
Biosurfactant	Linear	0.40	0.03	0.02	-2.62	Suggested
Biosurfactant	Quadratic	0.17	0.04	0.25	-6.91	Aliased
Pigment	Mean	0.0002				Suggested
	Linear	0.97	0.04	-0.23	-1.68
	Quadratic	0.31	0.04	-0.09	-6.56	Aliased

	Source	Sum of Squares	df	Mean Square	F-Value	p-value Prob > F
Biomass	Model	0	0
(OD600)	Residual	6.91	22	0.314
(Mean model)	Lack of Fit	6.84	18	0.38	20.97	0.005	Significant
	Pure Error	0.07	4	0.018
	Cor Total	6.91	22
	Model	1.61	5	0.32	1.09	0.403	Not significant
	Linear Mixture	1.61	5	0.32	1.09	0.403
Biosurfactant	Residual	5.02	17	0.30
(Linear model)	Lack of Fit	4.83	13	0.37	7.99	0.03	Significant
	Pure Error	0.19	4	0.05
	Cor Total	6.63	22
	Model	0	0
Pigment(Mean model)	Residual	1552.13	22	70.55
	Lack of Fit	1488.98	18	82.72	5.24	0.06	Not significant
	Pure Error	63.15	4	15.79
	Cor Total	1552.13	22

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