Endo-and exo-polygalacturonase (PG) production by Aspergillus oryzae IPT 301 was evaluated in a batch bioreactor in media containing 0, 10, and 20 g.L-1 pectin and 0 to 30 g.L-1 glucose. For cultivations in which the pH was not controlled, the use of 20 g.L-1 pectin and 10 g.L-1 glucose resulted in superior enzyme activities compared to control media with 0 and 10 g.L-1 of the inducer and the same glucose concentration. Maximum endo-PG and exo-PG activities were 125.0 and 76.3 U.mL-1, respectively. For cultivations in which the pH was controlled to a minimum value of 2.7, media containing 20 g.L-1 pectin and 20 to 50 g.L-1 glucose were tested and significant improvement of both activities was attained. Maximum enzyme activities (175.0 U.mL-1 for endo-PG and 103.0 U.mL-1 for exo-PG) were obtained in pH-controlled batch experiments with 30 g.L-1 glucose.
Aspergillus oryzae; Polygalacturonases; Glucose; Pectin; pH control
