Effect of cold chain interruptions on the shelf-life of fluid pasteurised skim milk at the consumer stage

Efeito de interrupções na cadeia de frio na vida útil de leite desnatado fluido e pasteurizado na etapa de consumidor

Abstract

This study aimed to verify the effect of time and temperature abuse on bacterial numbers in fluid pasteurized skim milk by simulating the real-life scenario, which usually occurs when cold chain is interrupted by consumers prior to consumption that affect the shelf-life of milk. Total three trials were carried out in this study. Thermal abuse was simulated with temperature fluctuations from 5 °C. In the first trial, the information about holding the milk samples for 8 hours at three different temperatures of 15 °C, 20 °C and 25 °C was obtained using a data logger to predict the effect of temperature abuse on the milk microbial quality. Further, in the second and third trial, the effect of temperature abuse on bacterial numbers was examined by holding milk at 5 °C and then shifts temperature to 25 °C for 8 h and 6 h. The pH was monitored during storage. The total bacterial count was examined by the Standard Plate Count (SPC). The mean air temperature had the greatest impact on milk temperature. It took 3.0 h, 3.9 h and 4.2 h to warm up when exposed to the temperatures of 15 °C, 20 °C and 25 °C, respectively. The holding time of 8 h at 25 °C showed that bacterial numbers (1 x 10⁵ CFU mL^-1) were higher after 14 days of storage, but control samples at 5 °C (< 1 x 10⁴ CFU mL^-1) were still within the acceptable level (5 x 10⁴ CFU mL^-1). A holding time of 6 h at 25 ^oC showed much higher bacterial numbers (1 x 10⁹ CFU mL^-1) compared to control samples (1 x 10⁷ CFU mL^-1) which were held at 5 °C for 11 days. The pH of the milk decreased with increasing bacterial growth during the extended storage time. The results show that temperature abuse has a significant effect on milk microbial stability and shelf life. It is important to maintain the milk temperature at 5 °C or less as the bacterial growth directly depend on increasing temperature and holding time, which pose the potential risk of microbial hazards leading to foodborne illness. Thus, consumers must understand the factors associated with the safe handling of milk to keep it safe to use before the expiry date.

Keywords:
Bacteria; Temperature abuse; Holding time; Spoilage; Shelf-life