We report on an assay of direct transfer of DNA into calli and seeds of Racosperma (ex-Acacia) mangium, using a bioballistic method. We observed transient expression of the GUS gene in the treated tissues
We report on an assay of direct transfer of DNA into calli and seeds of Racosperma (ex-Acacia) mangium, using a bioballistic method. We observed transient expression of the GUS gene in the treated tissues
NAA concentration (mM) | BA concentration (mM) | Adult leaves | Hypocotyls | Roots | |||
1 | 2 | 1 | 2 | 1 | 2 | ||
0 | 0 | - | 0 | - | 0 | - | 0 |
0 | 0.44 | + | 68.75 | + | 68.42 | + | 59.37 |
0 | 1.11 | + | 56.25 | ++ | 83.78 | - | 0 |
0 | 4.44 | + | 78.12 | ++ | 80.56 | ++ | 93.75 |
0 | 22.19 | ++ | 87.50 | ++ | 91.67 | ++ | 90.32 |
1.07 | 0.44 | ++ | 84.37 | n.d. | - | n.d. | - |
1.07 | 2.21 | ++ | 100.00 | ++ | 92.11 | n.d. | - |
1.07 | 6.66 | n.d. | - | +++ | 100.00 | + | 84.37 |
2.68 | 4.44 | ++ | 96.88 | +++ | 44.00 | ++ | 96.88 |
10.74 | 4.44 | ++ | 100.00 | ++ | 97.43 | ++ | 65.62 |