Stone et al., 20231818 Stone M., Bakkour S., Grebe E., Emperador D.M., Escadafal C., Deng X., et al. Standardized evaluation of Zika nucleic acid tests used in clinical settings and blood screening. PLoS Negl Trop Dis. 2023;17:e0011157.
|
Performance Evaluation |
Nucleic Acid Tests |
Nucleic acid amplification tests for DENV, CHIKV, and ZIKV |
1017 plasma samples from individuals with DENV and suspected ZIKV infection |
Sensitivity: 95.39%, Specificity: 100%. |
Grifols assays (single plex and multiplex) showed high sensitivity. Grifols transcription-mediated amplification (TMA) assays recommended for screening in arbovirus outbreak regions. |
Pereira et al., 20232323 Pereira G.M., Manuli E.R., Coulon L., Cortes M.F., Ramundo M.S., Dromenq L., et al. Performance Evaluation of VIDAS((R)) diagnostic assays detecting anti-chikungunya virus IgM and IgG antibodies: an international study. Diagnostics. 2023;13:2306.
|
Performance Evaluation |
ELISA |
ELISA for CHIKV IgM and IgG |
660 samples from individuals suspected of CHIKV infection |
IgM Sensitivity: >88%; IgG Sensitivity: 100%; Specificity: 100%. |
CHIKV IgG/IgM ELISA exhibited high sensitivity and specificity. Outperformed RDT in accuracy. |
Tsai et al., 20232424 Tsai J.J., Tsai C.Y., Lin P.C., Chen C.H., Tsai W.Y., Dai Y.C., et al. Correction to: comparing the performance of dengue virus IgG and IgG-capture enzyme-linked immunosorbent assays in seroprevalence study. BMC Infect Dis. 2023;23:401.
|
Performance Evaluation |
ELISA, IgG-capture ELISA |
DENV IgG ELISA, and IgG-capture ELISA |
232 serum or plasma samples, including late-convalesce ZIKV samples from Brazil. |
IgG ELISA Sensitivity: 94.9%; IgG ELISA Specificity: 100%; IgG-capture ELISA Sensitivity: 18.6%; IgG-capture ELISA Specificity: 100%. |
Compared DENV IgG and IgG-capture ELISAs using serum/plasma panels with flavivirus infections. IgG ELISA showed higher sensitivity overall, suggesting its suitability in seroprevalence studies and pre-vaccination screening for dengue vaccines. |
DiazGranados et al., 2021 2525 DiazGranados C.A., Bonaparte M., Wang H., Zhu M., Lustig Y., Schwartz E., et al. Accuracy and efficacy of pre-dengue vaccination screening for previous dengue infection with five commercially available immunoassays: a retrospective analysis of phase 3 efficacy trials. Lancet Infect Dis. 2021;21:529-36.
|
Performance Evaluation |
ELISA, Rapid Diagnostic Test (RDT) |
DENV IgG ELISA, and RDT |
3833 samples, 2486 IgG-RDT positive |
Sensitivity: 91.1%, Specificity: 92.8%, |
IgG RDT demonstrated exceptional sensitivity and specificity. Suitable for screening DENV history and pre-vaccination seropositivity. |
Ribeiro et al., 20211515 Ribeiro M.O., Godoy D.T., Fontana-Maurell M., Costa E.M., Andrade E.F., Rocha D.R., et al. Analytical and clinical performance of molecular assay used by the Brazilian public laboratory network to detect and discriminate Zika, Dengue and Chikungunya viruses in blood. Braz J Infect Dis. 2021;25:101542.
|
Performance Evaluation |
RT-PCR |
ZDC Molecular Assay for DENV, CHIKV, ZIKV |
269 plasma samples |
Sensitivity: 100%, Specificity: 100%. |
ZDC molecular RT-PCR assay exhibited high sensitivity and specificity for detecting DENV, CHIKV, and ZIKV. |
Morales et al., 20212626 Morales I., Rosenberger K.D., Magalhaes T., Morais C.N.L., Braga C., Marques E.T.A., et al. Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela. PLoS Negl Trop Dis. 2021;15:e0009336.
|
Performance Evaluation |
ELISA |
IgM, IgAM, and IgG ELISAs for ZIKV antibodies |
543 samples from patients in Brazil |
IgAM Sensitivity: 93.5%, IgAM Specificity: 85%, IgM Sensitivity: 30.3%, IgM Specificity: 93%, IgG Sensitivity: 72%, IgG Specificity: 100%. |
IgAM ELISA showed strong performance during co-circulation of ZIKV, DENV, and CHIKV; where IgG displayed strong performance compared to IgM in a population faced with co-infection. |
Pereira et al., 20212727 Pereira S.S., Andreata-Santos R., Pereira L.R., Soares C.P., Felix A.C., de Andrade P., et al. NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus. Int J Infect Dis. 2021;112:202-4.
|
Performance Evaluation |
ELISA |
NS1-based DENV IgG ELISA |
76 serum samples |
Sensitivity: 82%, Specificity: 93%. |
Dengue IgG ELISA test showed promising accuracy with a good sensitivity (82%) and specificity (93%), making it effective for spotting DENV-specific antibodies; no cross-reactivity with ZIKV NS1 was found. |
Kikuti et al., 20192828 Kikuti M., Cruz J.S., Rodrigues M.S., Tavares A.S., Paploski I.A.D., Silva M.M.O., et al. Accuracy of the SD BIOLINE Dengue Duo for rapid point-of-care diagnosis of dengue. PLoS One. 2019;14:e0213301.
|
Performance Evaluation |
RDT |
DENV NS1, IgM, IgG ELISA vs. RDT |
500 serum samples |
Sensitivity: 46.8%, Specificity: 96%. |
Dengue Duo RDT showed high specificity but lower sensitivity for NS1 or IgM. Cross-reactions were not assessed. |
Colombo et al., 20191616 Colombo T.E., Versiani A.F., Dutra K.R., Rubiato J.G.D., Galvao T.M., Negri Reis A.F., et al. Performance of CDC Trioplex qPCR during a dengue outbreak in Brazil. J Clin Virol. 2019;121:104208.
|
Performance Evaluation |
RT-PCR |
Trioplex real-time RT-PCR |
1656 serum samples |
Sensitivity: 95.39%, Specificity: 100%. |
Trioplex RT-PCR detects DENV, CHIKV, and ZIKV with high sensitivity and specificity. Recommended for molecular screening in Brazil. |