Occurrence of Leishmania DNA in urines of dogs naturally infected with leishmaniasis |
2007 |
Franceschi et al. |
AccuPrep®, Bioneer, Korea |
PCR |
kDNA |
25% |
|
Detection of Leishmania infantum DNA by fret-based real-time PCR in urine from dogs with natural clinical leishmaniosis |
2007 |
Solano-Gallego et al. |
High Pure PCR Template Preparation Kit (Roche Applied Science) |
qPCR |
kDNA |
47% |
|
Leishmania infantum DNA detection in urine from patients with visceral leishmaniasis and after treatment control |
2008 |
Fisa et al. |
High Pure PCR Template kit (Roche Molecular Biochemicals, Mannheim, Germany) |
Nested-PCR |
Genomic |
88% |
|
Urine sampling for real-time polymerase chain reaction-based diagnosis of canine leishmaniasis |
2008 |
Manna et al. |
QIAamp® Blood Kit (QIAGEN, Santa Clarita, CA.)
|
qPCR |
kDNA |
NA |
|
A urine-based polymerase chain reaction method for the diagnosis of visceral leishmaniasis in immunocompetent patients |
2008 |
Motazedian et al. |
Phenol-chloroform-isoamyl alcohol method |
PCR |
kDNA |
96.8% |
|
Polymerase Chain Reaction Detection of Leishmania kDNA from the Urine of Peruvian Patients with Cutaneous and Mucocutaneous Leishmaniasis |
2011 |
Veland et al. |
Phenol-chloroform-isoamyl alcohol method |
PCR |
kDNA |
20.9% |
|
A comparison of four DNA extraction protocols for the analysis of urine from patients with visceral leishmaniasis |
2014 |
Silva et al. |
Protocol 1 |
Proteinase K digestion in a lysis buffer followed by phenol-chloroform extraction |
|
|
|
Protocol 2
|
Absolute ethanol + Proteinase K digestion in a lysis buffer followed by phenol-chloroform extraction |
PCR |
kDNA |
72.7% |
Protocol 3 |
QIAamp® DNA Mini Kit (QIAGEN, Hilden, Germany) |
|
|
|
Protocol 4 |
Illustra™ Blood GenomicPrep Mini Spin Kit (GE, Amersham Place, Little Chalfont, Bucks, UK) |
|
|
|
Evaluation of urine for Leishmania infantum DNA detection by real-time quantitative PCR |
2016 |
Pessoa-e-Silva et al. |
Protocol 1 |
Wizard® Genomic DNA Purification Kit(Promega Corporation, Madison,WI, USA) |
|
|
|
Protocol 2
|
QIAamp® DNA Mini Kit, (QIAGEN Sample and Assay Technologies, Hilden, NRW, GER) |
qPCR |
kDNA |
NA |
Protocol 3 |
QIAamp® Viral RNA Mini Kit (QIAGEN Sample and Assay Technologies, Hilden, NRW, GER) |
|
|
|
Protocol 4 |
Modified Phenol-Chloroform |
|
|
|
Isolation and molecular characterization of Leishmania infantum in urine from patients with visceral leishmaniasis in Brazil |
2018 |
Lima Junior et al. |
10% SDS, proteinase K, choloroform, 100% ethanol and 70% ethanol |
RFLP-PCR |
ITS1 |
37% |
|
Immunodetection and molecular determination of visceral and cutaneous Leishmania infection using patients' urine |
2018 |
Mirzaei et al. |
QIAamp® DNA Mini Kit (QIAGEN, Valencia, CA, USA) |
PCR |
kDNA |
V92.8% C86.9% |
|