The epsilon toxin, produced by Clostridium perfringens, is responsible for enterotoxemia in ruminants and is a potential bioterrorism agent. In the present study, 15 regions of the toxin were recognized by antibodies present in the serum, with different immunodominance scales, and may be antigen determinants that can be used to formulate subunit vaccines.
Keywords:
Epsilon; Toxin; Enterotoxemia; Epitope; Mapping
| Epitope | Spots | Sequence | Number of aa | Mass (Da) | pI | GRAVYa a GRAVY indicates the solubility of the peptide and it is calculated by adding the hydropathy value for each residue and dividing by the length of the sequence. |
|---|---|---|---|---|---|---|
| 1 | 7 | YSIVNIVSPTNVIAK | 15 | 1617.9 | 8.59 | 0.787 |
| 2 | 11 | IAKEISNTVSNEMSK | 15 | 1650.8 | 6.14 | -0.533 |
| 3 | 16-26 | KASYDNVDTLIEKGR YNTKYNYLKRMEKYY PNAMAYFDKVTINPQ |
45 | 5459.1 | 9.26 | -1.100 |
| 4 | 37-38 | MNYLEDVYVGKALLT NDT |
18 | 2059.3 | 4.03 | -0.128 |
| 5 | 40 | GKALLTNDTQQEQKL | 15 | 1686.8 | 6.07 | -1.160 |
| 6 | 43 | QQEQKLKSQSFTSKN | 15 | 1780.9 | 9.70 | -1.947 |
| 7 | 45 | KSQSFTSKNTDTVTA | 15 | 1614.7 | 8.59 | -0.980 |
| 8 | 47 | SKNTDTVTATTTHTV | 15 | 1576.6 | 6.46 | -0.640 |
| 9 | 50-52 | TTTHTVGTSIQATAK FTVPFN |
21 | 2222.4 | 8.44 | 0.014 |
| 10 | 68-73 | LVPANTTVEVIAYLK KVNVKGNVKLVGQVS |
30 | 3181.8 | 9.83 | 0.470 |
| 11 | 78-79 | GSEWGEIPSYLAFPR DGY |
18 | 2044.2 | 4.14 | -0.644 |
| 12 | 86-88 | TVNKSDLNEDGTINI NGKGNY |
21 | 2266.4 | 4.56 | -1.119 |
| 13 | 93-98 | SAVMGDELIVKVRNL NTNNVQEYVIPVDKK |
30 | 3386.9 | 5.94 | -0.233 |
| 14 | 102 | DKKEKSNDSNIVKYR | 15 | 1824.0 | 9.40 | -2.120 |
| 15 | 104-106 | NDSNIVKYRSLSIKA PGIK |
19 | 2103.4 | 10.00 | -0.479 |
