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Medical Microbiology • Braz. J. Microbiol. 42 (2) • June 2011 • https://doi.org/10.1590/S1517-83822011000200003 linkcopy

Open-access Detection of Bartonella henselae in defibrinated sheep blood used for culture media supplementation

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    Bartonella henselae was detected in defibrinated sheep blood employed in supplementing a selective bacteria culture medium by nested PCR. We recommended that highly sensitive technical tests be run to ensure a sterile culture medium for Bartonella spp. isolation, since infected blood samples used in preparation could lead to false-positive results.

    Bartonella; Culture Media; False Positive Reactions; Polymerase Chain Reaction; Sheep-Blood Contamination

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    Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
    E-mail: bjm@sbmicrobiologia.org.br
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