Screening of <i>Trichoderma</i> isolates for their potential of biosorption of nickel and cadmium

Nongmaithem, Nabakishor; Roy, Ayon; Bhattacharya, Prateek Madhab

doi:10.1016/j.bjm.2016.01.008

home Sumário navigate_before Anterior Atual Seguinte navigate_next

home Sumário

Environmental Microbiology • Braz. J. Microbiol. 47 (2) • Apr-Jun 2016 • https://doi.org/10.1016/j.bjm.2016.01.008 linkcopy

Screening of Trichoderma isolates for their potential of biosorption of nickel and cadmium

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Fourteen Trichoderma isolates were evaluated for their tolerance to two heavy metals, nickel and cadmium. Three isolates, MT-4, UBT-18, and IBT-I, showed high levels of nickel tolerance, whereas MT-4, UBT-18, and IBT-II showed better tolerance of cadmium than the other isolates. Under nickel stress, biomass production increased up to a Ni concentration of 60 ppm in all strains but then decreased as the concentrations of nickel were further increased. Among the nickel-tolerant isolates, UBT-18 produced significantly higher biomass upon exposure to nickel (up to 150 ppm); however, the minimum concentration of nickel required to inhibit 50% of growth (MIC₅₀) was highest in IBT-I. Among the cadmium-tolerant isolates, IBT-II showed both maximum biomass production and a maximum MIC₅₀ value in cadmium stress. As the biomass of the Trichoderma isolates increased, a higher percentage of nickel removal was observed up to a concentration of 40 ppm, followed by an increase in residual nickel and a decrease in biomass production at higher nickel concentrations in the medium. The increase in cadmium concentrations resulted in a decrease in biomass production and positively correlated with an increase in residual cadmium in the culture broth. Nickel and cadmium stress also influenced the sensitivity of the Trichoderma isolates to soil fungistasis. Isolates IBT-I and UBT-18 were most tolerant to fungistasis under nickel and cadmium stress, respectively.

Keywords Heavy metals; Biosorption; Trichoderma; MIC₅₀; Fungistasis

Isolate	Growth rate at 48 h (mm)	Colony appearance on PDA
MT-1	53.2	Light green, granular sporulation in concentric ring; greenish white mycelia growth at the margin.
MT-4	71.7	Whitish sporulation with greenish tinge, submerged mycelia growth, olive green pigmentation of medium.
MT-7	66.3	Profuse mycelial growth white in color, scanty green sporulation intermingled within the mycelia.
MT-8	71.8	Greenish white sporulation with profuse mycelial growth at margin; light yellow pigmentation in the medium.
MT-11	38.0	Light green colony with profuse white fluffy mycelial growth at margin.
MT-13	72.8	Greenish white scanty sporulation; light yellow pigmentation in the medium.
MT-18	66.3	Dark green granular sporulation in concentric ring with scanty mycelial growth; light green sporulation at the edge of the colony
MT-21	64.8	Profuse, greenish white fluffy mycelial growth with little granular green sporulation at the center.
MT-23	68.7	Grayish white, fluffy mycelial growth profusely at margin; dark green sporulation in high amount.
MT-24	59.7	Colony character more or less similar to MT-23.
MT-25	38.0	Colony character more or less similar to MT-23 and MT-24.
IBT-I	58.3	Whitish submerged mycelial growth with greenish tinge at the edge of the colony, little yellow pigmentation of the medium.
IBT-II	54.4	Greenish white raised colony with fluffy mycelial growth, dark green sporulation intermingled within the mycelia at the center.
UBT-18	75.3	Dark green sporulation with whitish green fluffy mycelial growth in concentric rings.

Isolate	Growth rate of Trichoderma isolates (mm) at 48 h
	Concentration of nickel
	0 ppm	40 ppm	60 ppm	100 ppm	150 ppm	200 ppm
MT-1	33.3	38.3	36.6	35.6	34.3	7.0
MT-4	64.8	66.0	57.0	54.9	53.2	30.0
MT-7	36.3	35.3	33.6	31.6	27.0	0.0
MT-8	36.6	38.6	36.3	31.6	30.0	0.0
MT-11	38.0	37.3	35.0	27.3	26.3	0.0
MT-13	38.6	38.6	37.0	34.0	27.6	0.0
MT-18	41.2	42.1	40.0	37.7	33.0	28.2
MT-21	49.7	59.6	53.6	49.7	35.7	34.0
MT-23	29.3	36.3	34.3	31.3	23.0	20.1
MT-24	39.3	46.0	40.5	40.0	29.6	18.6
MT-25	38.0	38.0	37.3	36.6	29.3	0.0
IBT-I	44.4	55.2	53.1	50.1	48.0	41.4
IBT-II	49.8	55.6	55.0	51.2	26.3	17.3
UBT-18	68.7	70.8	60.4	57.9	44.5	20.3

Isolate	Growth rate of Trichoderma isolates (mm) at 72 h
	Concentration of cadmium
	0 ppm	40 ppm	60 ppm	100 ppm	150 ppm	200 ppm
MT-1	90.0	44.5	40.2	34.0	24.3	11.3
MT-4	90.0	62.6	58.3	56.2	48.0	44.7
MT-7	90.0	55.2	49.1	49.0	43.5	39.3
MT-8	90.0	43.6	39.7	36.4	28.9	25.5
MT-11	90.0	56.5	56.2	53.6	43.0	35.3
MT-13	90.0	40.2	34.4	33.9	25.9	24.3
MT-18	90.0	55.1	52.8	46.1	45.2	29.7
MT-21	90.0	51.9	42.1	42.4	38.8	32.9
MT-23	90.0	50.2	46.7	44.4	40.4	33.7
MT-24	90.0	62.2	58.1	50.6	42.8	32.4
MT-25	90.0	61.1	59.2	47.5	46.1	35.8
IBT-I	90.0	57.7	24.3	13.0	0.0	0.0
IBT-II	90.0	73.4	63.0	52.0	39.3	34.0
UBT-18	90.0	66.6	54.8	50.7	43.6	40.6

Isolates	Biomass production (mg)
	Concentration (ppm)
	0 ppm	40 ppm	60 ppm	100 ppm	150 ppm	200 ppm
MT-4	240.67^def	256.67^cde	225.67^efg	223.00^defg	200.33^fgh	165.33^h
UBT-18	321.67^ab	359.00^a	323.33^ab	303.33^bc	269.33^cd	166.67^h
IBT-I	215.33^efgh	225.00d^efg	210.33^efgh	196.33^fgh	181.67^gh	177.33^gh

Isolate	Biomass production (mg)
	Concentration (ppm)
	0 ppm	40 ppm	60 ppm	100 ppm	150 ppm	200 ppm
MT-4	240.34^d	147.01^e	120.34^efg	110.34^efg	100.36^efgh	60.34^h
UBT-18	269.70^d	147.03^e	131.70^ef	123.70^efg	86.70^fgh	79.70^gh
IBT-II	577.03^a	523.70^b	453.70^c	440.37^c	410.37^c	240.36^d

Nickel				Cadmium
Isolate	Linear equation	R ²	MIC₅₀ (ppm)	Isolate	Linear equation	R ²	MIC₅₀ (ppm)
MT-4	Y = 46.77 x − 81.21	0.896	638.90	MT-4	Y = 43.52 x − 30.61	0.886	71.16
UBT-18	Y = 73.07 x − 132.4	0.834	313.49	UBT-18	Y = 36.76 x − 14.66	0.934	57.41
IBT-I	Y = 32.22 x − 55.53	0.992	1884.93	IBT-II	Y = 56.18 x − 82.46	0.768	227.92

Germination of conidia (%)
Nickel					Cadmium
Isolate	0 ppm	50 ppm	100 ppm	150 ppm	Isolate	0 ppm	50 ppm	100 ppm	150 ppm
MT-4	91.73^ab	89.09^c	81.65^ef	79.99^f	MT-4	88.37^b	68.33^cde	63.22^ef	57.04^f
UBT-18	88.23^c	85.14^d	79.18^f	75.81^g	UBT-18	96.10^a	73.70^c	69.36^cde	66.83^de
IBT-I	93.73^a	89.67^bc	83.00^de	81.31^ef	IBT-II	97.30^a	73.53^cd	70.83^cd	50.59^g

vertical_align_top file_download show_chart

more_horizclose
- image
- translate
- link
- article
- vertical_align_top
- file_download
- show_chart
- image
- translate
- link
- article

location_on

Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
E-mail: bjm@sbmicrobiologia.org.br

rss_feed Acompanhe os números deste periódico no seu leitor de RSS

Acessibilidade / Reportar erro

[TFN1] SEM ± 1.23; LSD (p = 0.05) 3.50.

[TFN2] PDA, potato dextrose agar.