Detection of both vanA &amp; vanBgenes in vanA phenotypes of Enterococci by Taq Man RT-PCR

Mirzaei, Bahman; Babaei, Ryhane; Asiabar, Akbar Pour Dadash; Bameri, Zakaria

doi:10.1590/S1517-838246120131234

Acessibilidade / Reportar erro

Brasil

Brazilian Journal of Microbiology

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Medical Microbiology • Braz. J. Microbiol. 46 (1) • Jan-Mar 2015 • https://doi.org/10.1590/S1517-838246120131234 copy

Detection of both vanA & vanBgenes in vanA phenotypes of Enterococci by Taq Man RT-PCR

Authorship SCIMAGO INSTITUTIONS RANKINGS

Twenty seven isolates of vancomycin resistant Enterococci based on the disk diffusion and E- test have been screened; being found eight (0.3%) clinical isolates of vanA & vanB through Taq Man Real Time PCR assay. This study shows the presence of both vanA & vanB genotypes in vanA phenotypes clinical isolates in the three hospitals in Iran.

vanA & vanB genotypes; vancomycin; Taq Man Real Time PCR; Enterococci

Enterococci	Phenotypes		Genotypes

VREs	Disc diffusion zone diameter	(MIC) μg/mL	vanA gene	vanB gene	vanA & vanB genes
E. faecium	0(R)	> 256 μg/mL	P	N	N
E. faecium	0(R)	256 μg/mL	P	N	N
E. faecium	1(R)	256 μg/mL	P	N	N
E. faecium	0(R)	256 μg/mL	P	N	N
E. faecium	0(R)	256 μg/mL	P	N	N
E. faecium	8(R)	196 μg/mL	P	N	N
E. faecium	8(R)	196 μg/mL	P	N	N
E. faecium	12(R)	96 μg/mL	N	P	N
E. faecium	0(R)	> 256 μg/mL	P	P	P
E. faecium	0(R)	> 256 μg/mL	P	P	P
E. faecium	1(R)	256 μg/mL	P	P	P
E. faecalis	0(R)	> 256 μg/mL	P	P	N
E. faecalis	0(R)	256 μg/mL	P	P	N
E. faecalis	12(R)	128 μg/mL	P	P	N
E. faecalis	11(R)	128 μg/mL	P	P	N
E. faecalis	11(R)	128 μg/mL	P	P	N
E. faecalis	14 (R)	64 μg/mL	N	P	N
E. faecalis	14 (R)	64 μg/mL	N	P	N
E. faecalis	14(R)	32 μg/mL	N	P	N
E. faecalis	14(R)	32 μg/mL	N	P	N
E. faecalis	14(R)	32 μg/mL	N	P	N
E. faecalis	12 (R)	96 μg/mL	N	P	N
E. faecalis	8(R)	196 μg/mL	P	P	P
E. faecalis	0(R)	256 μg/mL	P	P	P
E. faecalis	0(R)	> 256 μg/mL	P	P	P
E. faecalis	1(R)	256 μg/mL	P	P	P
E. faecalis	6(R)	196 μg/mL	P	P	P

van primers	Forward (5′-3′)	Reverse (5′-3′)
vanA primer:	TGGAGCGACAGACATAACAGAT	ACACCTACGGGCGAGTTTC
vanA probe	JOE-TATTATTGCTCGTTTACCGTA -BHQ1
vanB primer:	TGATTGTCGGCGAAGTGGAT	GCGTGGATAGCGGCTGTA
vanB probe:	HEX- TCAGAGAATGCGATGATTATC-Tamra

Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
E-mail: bjm@sbmicrobiologia.org.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] Send correspondence to Z. Bameri. Research Zahedan University of Medical Science Booali Hospital, Zahedan, Iran. E-mail: zakariabameri@gmail.com.