Multi-enzyme complex of white rot fungi in saccharification of lignocellulosic material

Cardoso, Wilton Soares; Queiroz, Paula Viana; Tavares, Gabriella Peterlini; Santos, Fernando Almeida; Soares, Filippe Elias de Freitas; Kasuya, Maria Catarina Megumi; Queiroz, José Humberto de

doi:10.1016/j.bjm.2018.05.006

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Short communication • Braz. J. Microbiol. 49 (4) • Oct-Dec 2018 • https://doi.org/10.1016/j.bjm.2018.05.006 linkcopy

Multi-enzyme complex of white rot fungi in saccharification of lignocellulosic material

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ABSTRACT

The multi-enzyme complex (crude extract) of white rot fungi Pleurotus ostreatus, Pleurotus eryngii, Trametes versicolor, Pycnosporus sanguineus and Phanerochaete chrysosporium were characterized, evaluated in the hydrolysis of pretreated pulps of sorghum straw and compared efficiency with commercial enzyme. Most fungi complexes had better hydrolysis rates compared with purified commercial enzyme.

Keywords: Enzymes; Cellulases; Fungus; Hydrolysis

Fungi	Celullase total (FPase) (U g^-1)	AVICELase (U g^-1)	CMCase (U g^-1)	β-Glucosidase (U g^-1)	Xylanase (U g^-1)	Laccase (U g^-1)	Protein mg g^-1 colonized substrate
P. sanguineus PYC02	0.80 ± 0.05	0.88 ± 0.08	2.03 ± 0.18	0.38 ± 0.03	8.43 ± 0.00	14.33 ± 0.57	1.33 ± 0.13
P. ostreatus PLO06	1.32 ± 0.03	4.12 ± 0.02	2.25 ± 0.07	0.18 ± 0.01	1.33 ± 0.00	5.65 ± 0.42	1.67 ± 0.14
P. eryngii PLE04	0.65 ± 0.01	3.12 ± 0.02	1.43 ± 0.05	0.23 ± 0.03	1.80 ± 0.10	6.58 ± 0.68	2.30 ± 0.20
P.chrysosporium PC	1.13 ± 0.04	0.65 ± 0.04	4.03 ± 0.10	1.08 ± 0.04	2.37 ± 0.15	0.70 ± 0.08	1.96 ± 0.10
T. versicolor TRAM01	1.02 ± 0.05	1.43 ± 0.07	2.25 ± 0.10	1.88 ± 0.05	8.03 ± 0.01	9.87 ± 0.53	1.65 ± 0.12

Activities of crude extracts	Apparent optimal parameter	Fungi
		PC	PYC	TRAM01	PLE04	PLO06
Cellulase total (FPAse)	pH	5.0	5.0	4.0	7.0	7.0
	Temperature	60 ºC	70 ºC	50 ºC	50 ºC	60 ºC
	Thermostability^a	40%	35%	35%	20%	10%
CMCase	pH	4.0	4.0	4.0	7.0	5.0
	Temperature	50 ºC	50 ºC	50 ºC	50 ºC	60 ºC
	Thermostability^a	30%	50%	60%	0%	0%
Avicelase	pH	6.0	6.0	6.0	6.0	6.0
	Temperature	50 ºC	50 ºC	50 ºC	50 ºC	50 ºC
	Thermostability^a	20%	0%	25%	0%	0%
β-Glucosidase	pH	7.0	7.0	5.0	5.0	5.0
	Temperature	50 ºC	70 ºC	60 ºC	40 ºC	40 ºC
	Thermostability^a	40%	40%	50%	80%	85%

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Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
E-mail: bjm@sbmicrobiologia.org.br

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