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Genetics and Molecular Microbiology • Braz. J. Microbiol. 45 (2) • June 2014 • https://doi.org/10.1590/S1517-83822014000200044   copy

An improved purification procedure for Leishmania RNA virus (LRV)

Authorship SCIMAGO INSTITUTIONS RANKINGS

Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations.

Leishmania guyanensis; Leishmania RNA virus; LRV; virus isolation; electron microscopy

Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
E-mail: bjm@sbmicrobiologia.org.br
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