Ototoxicity of cypermethrin in Wistar rats

Cunha, Eduarda Oliveira; Reis, Aléxia dos; Macedo, Mateus Belmonte; Machado, Márcia Salgado; Dallegrave, Eliane

doi:10.1016/j.bjorl.2019.02.007

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Original articles • Braz. j. otorhinolaryngol. 86 (5) • Sep-Oct 2020 • https://doi.org/10.1016/j.bjorl.2019.02.007 copy

Ototoxicity of cypermethrin in Wistar rats^☆ ☆ Please cite this article as: Cunha EO, Reis A, Macedo MB, Machado MS, Dallegrave E. Ototoxicity of cypermethrin in Wistar rats. Braz J Otorhinolaryngol. 2020;86:587-92.

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Introduction:

This study presents the effect of cypermethrin on the cochlear function in Wistar rats post-subchronic inhalation exposure. Worldwide several pesticides are described as causing health disorders. Cypermethrin is currently one of the most commonly used, however, little is known about its harmful effects, especially related to hearing. Human studies have associated pesticides with hearing disorders, but they present limited conclusions due to the multiple factors to which the population is exposed, such as noise.

Objective:

Mimic human exposure to cypermethrin and to verify the effects on cochlear function.

Methods:

It is a subchronic inhalation animal study (6 weeks, 4 hours/day), using 36 male Wistar aged 60 day. Rats were randomly assigned into three groups: Control (12 rats exposed to inhalation of water); Positive Control for auditory lesion (12 rats administrated with 24 mg/kg intraperitoneal cisplatin); Experimental (12 rats exposed to inhalation of cypermethrin - 0.25 mg/L). Animals were evaluated by distortion product otoacoustic emissions pre- and post-exposure.

Results:

The frequencies of 8, 10 and 12 kHz in both ears (right p = 0.003; 0.004; 0.008 and left 0.003; 0.016; 0.005 respectively) and at frequencies 4 and 6 in the right ear (p = 0.007 and 0.015, respectively) in the animals exposed to cypermethrin resulted in reduction.

Conclusion:

Subchronic inhalation exposure to cypermethrin provided ototoxicity in rats.

KEYWORDS
Ototoxicity; Cypermethrin; Rats

Ear	Frequency (kHz)	Pre-exposure median (quartile25/quartile75)	Post-exposure median (quartile25/quartile75)	p-Value (Mann-Whitney)
Right	4	9.50 (3.50/17.25)	-2.50 (-9.25/1.00)	0.007^a a p < 0.05 (Mann-Whitney).
Right	6	27.50 (6.50/38.50)	0.50 (-10.75/10.25)	0.015^a a p < 0.05 (Mann-Whitney).
Right	8	31.50 (16.75/46.25)	-2.00 (-25.00/14.75)	0.003^a a p < 0.05 (Mann-Whitney).
Right	10	27.50 (2.00/42.25)	0.00 (-6.50/6.75)	0.004^a a p < 0.05 (Mann-Whitney).
Right	12	10.00 (7.00/44.00)	0.50 (-11.50/5.25)	0.008^a a p < 0.05 (Mann-Whitney).
Left	4	6.50 (4.50/14.00)	4.00 (-2.75/9.00)	0.146
Left	6	8.00 (00.00/23.00)	1.00 (-3.75/3.75)	0.077
Left	8	9.50 (7.00/33.25)	17.00 (-25.00/-1.75)	0.003^a a p < 0.05 (Mann-Whitney).
Left	10	10.50 (5.00/29.50)	-0.50 (-14.50/7.25)	0.016^a a p < 0.05 (Mann-Whitney).
Left	12	29.00 (10.75/34.00)	6.00 (2.50/9.00)	0.005^a a p < 0.05 (Mann-Whitney).

Ear	Frequency (KHz)	Pre-exposure median (quartile25/quartile75)	Post-exposure median (quartile25/quartile75)	p-Value (Mann Whitney)
Right	4	7.00 (4.00/12.25)	4.00 (-2.50/8.00)	0.116
Right	6	11.50 (3.50/23.50)	3.00 (0.00/7.25)	0.131
Right	8	28.00 (11.50/35.00)	18.00 (7.00/33.50)	0.195
Right	10	24.00 (12.50/34.25)	7.50 (3.25/28.00)	0.050
Right	12	24.00 (17.25/36.00)	7.00 (1.25/22.25)	0.006^a a p < 0.05 (Mann-Whitney).
Left	4	6.50 (2.25/9.25)	-0.50 (-2.25/3.25)	0.032^a a p < 0.05 (Mann-Whitney).
Left	6	14.00 (10.75/20.50)	7.00 (1.50/14.00)	0.136
Left	8	23.50 (18.00/28.25)	16.00 (2.00/29.25)	0.195
Left	10	25.50 (16.75/30.25)	12.00 (5.50/29.50)	0.147
Left	12	20.50 (8.00/38.50)	8.00 (4.00/26.00)	0.239

Ear	Frequency (kHz)	Pre-exposure median (quartile25/quartile75)	Post-exposure median (quartile25/quartile75)	p-Value (Mann-Whitney)
Right	4	6.50 (2.25/13.00)	10.50 (7.50/14.50)	0.255
Right	6	27.50 (15.25/33.25)	33.50 (15.00/36.25)	0.646
Right	8	28.00 (10.50/35.25)	36.50 (21.50/39.00)	0.505
Right	10	32.00 (14.00/38.00)	38.00 (34.50/43.25)	0.308
Right	12	29.50 (16.25/39.25)	42.00 (21.75/48.00)	0.195
Left	4	2.00 (0.00/12.50)	11.00 (3.75/19.50)	0.266
Left	6	16.50 (09.75/26.75)	26.00 (24.00/32.75)	0.209
Left	8	19.00 (4.25/37.50)	38.00 (29.25/39.75)	0.153
Left	10	29.00 (19.00/35.25)	30.50 (24.50/39.50)	0.724
Left	12	31.50 (25.50/39.75)	42.50 (26.50/45.50)	0.213

Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial. Sede da Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico Facial, Av. Indianópolia, 1287, 04063-002 São Paulo/SP Brasil, Tel.: (0xx11) 5053-7500, Fax: (0xx11) 5053-7512 - São Paulo - SP - Brazil
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[1] * Corresponding author. E-mail: elianedal@ufcspa.edu.br (E. Dallegrave).

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Ototoxicity of cypermethrin in Wistar rats☆ ☆ Please cite this article as: Cunha EO, Reis A, Macedo MB, Machado MS, Dallegrave E. Ototoxicity of cypermethrin in Wistar rats. Braz J Otorhinolaryngol. 2020;86:587-92.

Abstract

Introduction:

Objective:

Methods:

Results:

Conclusion:

Ototoxicity of cypermethrin in Wistar rats^☆ ☆ Please cite this article as: Cunha EO, Reis A, Macedo MB, Machado MS, Dallegrave E. Ototoxicity of cypermethrin in Wistar rats. Braz J Otorhinolaryngol. 2020;86:587-92.