Spontaneous healing of the tympanic membrane after
               traumatic perforation in rats

Araújo, Marcos Miranda de; Murashima, Adriana Andrade Batista; Alves, Vani Maria; Jamur, Maria Celia; Hyppolito, Miguel Angelo

doi:10.1016/j.bjorl.2014.05.014

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Brazilian Journal of Otorhinolaryngology

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Sumário

Original Articles • Braz. j. otorhinolaryngol. 80 (4) • Jul-Aug 2014 • https://doi.org/10.1016/j.bjorl.2014.05.014 copy

Spontaneous healing of the tympanic membrane after traumatic perforation in rats ^☆ ☆ Please cite this article as: Araújo MM. Murashima AA, Alves VM, Jamur MC, Hyppolito MA. Spontaneous healing of the tympanic membrane after traumatic perforation in rats. Braz J Otorhinolaryngol 2014;80:330-8. ^☆☆ ☆☆ Institution: Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, São Paulo, SP, Brazil.

Authorship SCIMAGO INSTITUTIONS RANKINGS

OBJECTIVE:

The objective of the present study was to assess the healing of traumatic tympanic membrane perforation in rats.

METHODS:

The tympanic membrane from male Wistar rats was perforated in the anterior and posterior portions to the handle of the malleus. Five tympanic membranes were evaluated 3 days after tympanic perforation; 5 after 5 days; 5 after 7 days; 3 after 10 days; and 4 after 14 days. The tympanic membranes were submitted to histopathological evaluation after hematoxylin-eosin staining.

RESULTS:

Tympanic membrane closure occurred at about 7-10 days after injury and the healing process was complete by day 14. The proliferative activity of the outer epithelial layer was present close to the handle of the malleus and to the tympanic annulus.

CONCLUSION:

The spontaneous healing process of the tympanic membrane starts from the outer epithelial layer, with later healing of the lamina propria and the mucosal layer.

Tympanic membrane perforation; Wound healing; Histology

Time of euthanization (days)	Epithelial layer, µm (%)	Lamina propria layer, µm (%)	Mucosal layer, µm (%)	Total, µm (100%)
Intact TM^a	1.8 (18.7%)	6.3 (65.6%)	1.5 (15.6%)	9.6
Intact TM^b	2.6 (16.4%)	11.9 (75.3%)	1.3 (8.2%)	15.8
Intact TM^c	2.3 (8.6%)	22.3 (83.8%)	2 (7.5%)	26.6
3 days^a	18.9 (48.4%)	14.3 (36.7%)	5.8 (14.9%)	39.0
5 days^a	27.1 (37.9%)	36.2 (50.7%)	8.1 (11.3%)	71.4
7 days^a	37.5 (42.1%)	46.1 (51.7%)	5.5 (6.1%)	89.1
10 days^a	11.2 (29.9%)	23.3 (62.1%)	3 (8.0%)	37.5
14 days^a	5.7 (27.9%)	12.5 (61.3%)	2.2 (10.8%)	20.4

Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial. Sede da Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico Facial, Av. Indianópolia, 1287, 04063-002 São Paulo/SP Brasil, Tel.: (0xx11) 5053-7500, Fax: (0xx11) 5053-7512 - São Paulo - SP - Brazil
E-mail: revista@aborlccf.org.br

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[1] * Corresponding author.E-mails: drmarcosorl@usp.br, drmarcosorl@hotmail.com (M.M. Araujo)