Evaluation of inner ear damage by mastoid drilling with measurement of serum prestin (SLC26A5) levels

Abstract

Objective

The objective of this study is to demonstrate any inner ear injury caused by drilling in mastoid surgery with prestin, outer hair cell motor protein specific to the cochlea.

Methods

The patients with chronic otitis media requiring mastoidectomy (n= 21) and myringoplasty (n= 21) were included. Serum prestin level obtained from blood samples was measured before surgery and on postoperative days 0, 3, and 7 using Human Prestin (SLC26A5) ELISA Kit. All patients underwent the Pure Tone Audiometry (PTA) test before surgery and on the postoperative 7th day. The drilling time was also recorded for all patients who underwent mastoidectomy.

Results

In both mastoidectomy and myringoplasty groups, the postoperative serum prestin levels increased on days 0 and 7 (p_day-0 = 0.002, p_day-7 = 0.001 and p_day-0 = 0.005, p_day-7 = 0.001, respectively). There was no significant difference in the serum prestin levels between the two groups, postoperatively. The PTA thresholds at day 7 did not change in either group. A significant decline at 2000 Hz of bone conduction hearing threshold in both groups and a decline at 4000 Hz in the myringoplasty group were found. There was no correlation between the drilling time and the increase of prestin levels in the postoperative day 0, 3, and 7.

Conclusion

Our results showed that mastoid drilling is not related to a significant inner ear injury. Although the myringoplasty group was not exposed to drill trauma, there was a similar increase in serum prestin levels as the mastoidectomy group. Also, a significant decline at 2000 Hz of bone conduction hearing threshold in both groups and a decline at 4000 Hz in the myringoplasty group were found. These findings suggest that suction and ossicular manipulation trauma can lead to an increase in serum prestin levels and postoperative temporary or permanent SNHL at 2000 and 4000 Hz.

Level of evidence: Level-4.

Keywords
Prestin; SLC26A5; Mastoidectomy; Inner ear; Drill

Highlights

Mastoid drilling is not related to a significant inner ear injury.

Suction and ossicular manipulation trauma can increase serum prestin levels.

Suction trauma can cause sensorineural hearing loss at 2000 and 4000 Hz.

Ossicular manipulation trauma can cause sensorineural hearing loss at 2000 and 4000 Hz.