Comprehensive analysis of the immune implication of EPHX4 gene in laryngeal squamous cell carcinoma

Abstract

Objectives:

The role of Epoxide Hydrolase-4 (EPHX4), a member of epoxide hydrolase family, has not been investigated in cancer. The purpose of this article is to explore the application value of EPHX4 in laryngeal cancer and its relationship with immune infiltration.

Methods:

We observed that EPHX4 expression and its survival assays in laryngeal cancer specimens based on The Cancer Genome Atlas (TCGA) cohorts. We also analyzed the correlation between immune cell infiltration levels and EPHX4 gene copy number in laryngeal cancer. Finally, we conducted in vitro assay to evaluate the functions of EPHX4 in laryngeal cancer cell line.

Results:

EPHX4 is highly expressed in laryngeal cancer specimens and has a poor prognosis. EPHX4 related immune cell analysis showed that it participated in NK Natural killer cell mediated cytotoxicity. Finally, Cell experiments indicate that EPHX4 could promote laryngeal cancer cell line proliferation, colony formation and invasion.

Conclusions:

Our research results suggest that EPHX4 may be a potential immunotherapy target for laryngeal cancer. The nominated immune signature is a helpful and promising prognostic indicator in laryngeal cancer.

Levels of Evidence: Level 3.

Keywords
Laryngeal cancer; EPHX4; Immunotherapeutic

HIGHLIGHTS

The expression level of EPHX4 was higher in HNSC patients’ tissues.

EPHX4 expression is closely associated with the immunity of HNSC.

EPHX4 promotes HNSC cells proliferation, colony formation and invasion.