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Genetic dissimilarity (Avena Sativa L.) tolerant and sensitive mutants to organic acids

In order to cultivate oats (Avena sativa L.) as an economically alternative in low lands, it is necessary to use cultivars tolerant to the main organic acids produced by organic matter decomposition resulting from no tillage systems. The objectives of this work were estimate genetic dissimilarity among m3 mutant families and the oat cultivar UFRGS 14 using ISSR markers; and to detect genomic regions associated with organic acids toxicity. Thirty M3 mutant families from the Plant Genomic Center of the College of Agronomy "Eliseu Maciel", derived from the cultivar UFRGS 14 irradiated with gamma ray (Co60) at 400 Gy dosage were used. The genetic characterization was carried out using the ISSR marker technique. The results indicated that UBC 854, 855 and 811 primers allowed the identification of genomic regions related to organic acid toxicity tolerance, while UBC 850 and 826 primers of genomic regions associated to organic acid sensitivity.

molecular markers; ISSR marker; gamma ray


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