Leão et al., 2008 |
Embryo |
Bovine |
Vitrification -100 µM 9c, 11t and 10t, 12c CLA |
Supplementation with CLA in in vitro production increased the survival of the vitrified and thawed embryos after 3 hours (80.0%) compared with the control (63.8%). |
Supplementation of the media with cis- and trans-9,11- and - 10,12- CLA during IVM and IVC increased the rate of embryonic survival after warming. |
Pereira et al., 2008 |
Embryo |
Bovine |
Vitrification-100 µM 10t, 12c CLA |
The addition of a conjugated isomer of linoleic acid, the trans-10, cis-12 to embryo culture, improved embryo post thawing viability after (68,6% / 27,4%). |
CLA containing media decreased embryo cytoplasmic lipid deposition and improved embryo resistance to micromanipulation and cryopreservation. |
Soares et al., 2013 |
Sperm |
Bovine |
Conventional 50µM, 100µM, 150µM 9c, 11t and 10t, 12c CLA |
There was no difference between treatments. |
The use of CLA in the dilution medium of bovine sperm showed the highest values of average speed in 50 µM and a higher percentage of membrane intake at 100 µM. |
Batista et al., 2014 |
Embryo |
Bovine |
Conventional -100 µM 10t, 12c CLA |
A significant increase was observed in the re-expansion rate of embryos cultured with trans-10, cis-12 CLA when compared to control (56.3 vs. 34.4%). However, this difference was not observed in the hatching rate (16.5 vs. 14.0%). |
The mechanism by which trans-10, cis-12 CLA reduces the neutral lipid content of in vitro produced embryos involves a down-regulation in the expression of the 1- acyiglycerol-3-phosphate 0- acyltransferase enzyme. |
Absalón- Medína et al. 2014 |
Embryo |
Bovine |
Vitrification - 9c,11t and 10t,12c were tested at high (50,100, and 200 µM) and low (15 and 25 µM) |
Higher survival rate after rewarming and culture, and a higher embryo blastomere count was noted. |
Embryos cultured in CLA containing media showed higher survival rates after cryopreservation. |
Matos et al., 2015 |
Oocyte |
Bovine |
Maturation-100 µM 10t, 12c CLA- Vitrification EG/DMSO 10%. |
CLA supplementation improved the developmental competence of vitrifed/warmed. Cryoprotectants exposition reduced oocytes membrane permeability (37% - 42%). |
CLA supplementation improves post-freezing oocyte viability. |
Teixeira et al., 2017 |
Sperm |
Porcine |
Conventional - 50µM 10t, 12c |
Viability decreased significantly after freezing and thawing. |
There was no observed advantages on the post-thaw boar sperm viability and integrity. |
Karimi et al., 2017 |
Sperm |
Bovine |
Conventional - 5g by gavage using 9c, 11t and 10t, 12c CLA |
Lower values in both treatments at weeks 6 and 8, with a recovery by week 10. |
CLA supplementation does not change sperm progressive motility in fresh and frozen/thawed samples. |
Carvalho et al., 2019 |
Embryo |
Bovine |
Vitrification-100 µM 10t, 12c CLA |
Improves hatching rates when added throughout the growth. |
CLA supplementation promotes the reduction of lipid levels and improved cryoresistance. |
Dias et al., 2020 |
Embryo |
Bovine |
Slow Freezing -100 µM 10t, 12c CLA |
Reduction in the rate of blastocysts with an reexpansion rate of 43% |
CLA during in vitro culture was not able to improve embryo production and response to cryopreservation. |