In vitro germination of 'Murmuru' zygotic embryos (Astrocaryum ulei)The work aimed to evaluate the influence of sucrose concentrations and seed physiologic age on the in vitro germination of murmuru zygotic embryos. Fruits collected from field plants in two development stages had the embryos excised, desinfected and inoculated onto MS medium with 75% salts, 2.5 g.L-1 of giberelic acid and different sucrose concentrations: 15, 30 and 45 g.L-1. All cultures were kept for 30 days in the dark at 25±2ºC, being transferred afterwards to a growth chamber with 30 mmol.m-2s-1 of radiation and 16 hours photoperiod for complete development. The statistical design was a 2x3 factorial, with six repetitions and five embryos by experimental unit. After 30 days it was evaluated the germination percentage and plantlets height. It was verified that embryos from immature fruits presented higher germination percentage and the sucrose concentration of 30 g.L-1 was the one that provided the best results among the others tested. Embryos from mature fruits gave rise to plantlets with average height significantly greater than that of plantlets from immature fruits and 30 g.L-1 of sucrose was the concentration that provided the best results.
Astrocaryum spp.; palm; murumuru; embriogenesis; carbohydrate; tissue culture
