The aim of this work was to analyse the genetic diversity among twenty seven access of cassava (Manihot esculenta) from Embrapa Eastern Amazon Germoplasm Collection. All twelve RAPD primers utilized to amplify the DNA showed polimorfism in Manihot esculenta. From a total of two hundred and eleven bands, it was selected seventy three polimorphic bands. Genetic divergence analysis was carried out by NTSYS-pc, 2.02 and Jaccard coefficient. The dendogram analysis showed two different groups. The first group, which was splited in two small groups (genetic similarity varied from 8 % to 78 %), included twenty access originated of different locals. The second group was split in two small groups (genetic similarity varied from 9 % to 38 %). The access CPATU 260 and CPATU 137 were shown to be the most divergent and to belong to distinct groups. In conclusion, the results showed genetic diversity that can be used in breeding programs and in strategies to manage germoplasm collection of cassava.
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