Plant growth regulators and organic additives on the proliferation of protocorm-like bodies and plantlet regeneration of <i>Cattleya gaskelliana</i> (N.E.Br.) B.S.Williams

Hussien, Muthab; Molkanova, Olga Ivanovna; Raeva-Bogoslovskaya, Ekaterina Nikolaevna; Sergeevich, Makarov Sergey

doi:10.1590/1413-7054202448018223

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Agricultural Sciences • Ciênc. agrotec. 48 • 2024 • https://doi.org/10.1590/1413-7054202448018223 copy

Plant growth regulators and organic additives on the proliferation of protocorm-like bodies and plantlet regeneration of Cattleya gaskelliana (N.E.Br.) B.S.Williams

Reguladores de crescimento vegetal e aditivos orgânicos na proliferação de corpos semelhantes a protocormos e na regeneração de plântulas de Cattleya gaskelliana (N.E.Br.) B.S.Williams

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

In this study, a tissue culture method was developed for the propagation and conservation of Cattleya gaskelliana (N.E.Br.) B.S. Williams. Initially, protocorms with leaf primordium, obtained via asymbiotic seed germination, were used as explants. Half-strength Murashige and Skoog (MS) medium supplemented with 1.5 mg L⁻¹ 6-benzyl aminopurine (BA) and 2.0 mg L⁻¹, indole butyric acid (IBA) was found to be optimal for the proliferation of protocorm-like bodies (PLB). Most explants (81%) proliferated, each producing 11.1 ± 1.87 units per explant. The optimum number of PLB differentiated to plantlets on a cultured medium with 0.5 mg L⁻¹ Thidiazuron (TDZ) + 100 mL L⁻¹ coconut water. Firmly rooted plantlets with vigorous growth were regenerated on ½ MS medium + 1.0 mg L⁻¹ Indole- 3-acetic acid (IAA) and 50 g L⁻¹ banana puree. Plants regenerated via in vitro processes were wrapped with sphagnum moss and acclimatized in a substrate containing equal proportions of bark, perlite, and peat with a 100% ex-vitro survival rate after acclimation for 70 days. The protocol developed in this study can be used to obtain several thousand plants within one year, and thus, is an effective method.

Index terms:
Orchids; PLB; auxin; cytokinin; organic extract.

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[1] ^*Corresponding author: muthab.hussien95@gmail.com

Cytokinin 0.5 mg L^-1	Organic additives	Treatment
BA	100 mL L⁻¹ Coconut water	A
BA	50 g L⁻¹ Banana puree	B
TZD	100 mL L⁻¹ Coconut water	C
TZD	50 g L−1 Banana puree	D
Kin	100 mL L⁻¹ Coconut water	E
Kin	50 g L−1 Banana puree	F

Treatment	Plantlet height (cm)	Number of leaves (unit)	Number of adventitious shoots (unit)
A	1.24 ± 0.28 b	3.30 ± 0.87 ab	4.70 ± 0.44 ab
B	0.99 ± 0.15 bc	2.60 ± 0.38 bc	5.20 ± 1.01 b
C	1.65 ± 0.13 a	3.60 ± 0.37 a	6.50 ± 0.47 a
D	0.60 ± 0.11 de	2.10 ± 0.22 c	4.00 ± 0.87 bc
E	0.82 ± 0.29 cd	2.30 ± 0.25 c	2.40 ± 0.80
F	0.50 ± 0.17 e	2.10 ± 0.22 c	2.30 ± 0.90 c

Treatment	Plantlet height (cm)	Number of roots (unit)	Number of leaves (unit)	Root length(cm)	Rooting (%)	Number of adventitious shoots (unit)
A	2.78 ± 0.30 a	4.78 ± 1.28 ab	6.14 ± 0.89 a	4.10 ± 0.97 a	95.0 ± 8,36 a	1.64 ± 0.28 cdef
B	1.90 ± 0.26 c	3.57 ± 1.51 bc	5.42 ± 1.27 a	3.12 ± 0.75 bc	91.7 ± 6.83 ab	1.48 ± 0.32 ef
C	2.00 ± 0.31 c	4.71 ± 1.70 ab	5.28 ± 0.75 ab	3.22 ± 0.37 ab	90.0 ± 7.07 ab	1.83 ± 0.46 bcdef
D	2.22 ± 0.34 bc	4.85 ± 1.86 a	4.85 ± 0.69 abc	2.92 ± 0.26 bcd	88.33 ± 6.83 ab	4.10 ± 0.50 a
E	1.84 ± 0.57 c	2.28 ± 0.95 cde	3.42 ± 0.53 cd	2.28 ± 0.26 cdef	70.8 ± 8.61 c	2.40 ± 0.56 bc
F	2.08 ± 0.31 c	2.28 ± 0.48 cde	3.57 ± 0.78 cd	2.04 ± 0.39 def	68.3 ± 6.05 cd	2.45 ± 0.73 b
G	2.05 ± 0.39 c	1.28 ± 0.47 e	3.85 ± 0.69 bcd	1.57 ± 0.21 f	51.7 ± 7.5 e	1.31 ± 0.24 f
H	2.18 ± 0.24 bc	1.85 ± 0.81 de	3.42 ± 1.24 cd	1.96 ± 0.36 ef	60.0 ± 7.07 de	1.50 ± 0.32 ef
I	2.64 ± 0.65 ab	3.14 ± 1.06 cd	3.85 ± 0.89 bcd	3.24 ± 0.64 ab	84.0 ± 4.7 b	2.27 ± 0.55 bcde
J	1.88 ± 0.27 c	2.17 ± 0.37 de	3.57 ± 0.53 cd	2.79 ± 0.66 bcde	83.3 ± 6.6 b	1.62 ± 0.36 def
K	2.21 ± 0.51 bc	2.42 ± 0.53 de	3.28 ± 0.48 d	2.22 ± 0.31 cdef	85.0 ± 8.9 b	1.57 ± 0.32 ef
L	2.37 ± 0.54 abc	2.85 ± 0.69 cd	3.42 ± 0.53 cd	3.08 ± 0.32 bc	83.3 ±8.7 b	2.36 ± 0.28 bcd

BA (mg L^-1)	IBA (mg L^-1)
BA (mg L^-1)	1.0	1.5	2.0
1.0	+	+
1.5	+	+	+
2.0		+	+

Organic additives	Auxin type	Auxin (mg L⁻¹)	Treatment
Banana puree 50 g L⁻¹	IAA	1.0	A
	IAA	2.0	B
	IBA	1.0	C
	IBA	2.0	D
Potato puree 20 g L⁻¹	IAA	1.0	E
	IAA	2.0	F
	IBA	1.0	G
	IBA	2.0	H
Coconut water 100 mL L⁻¹	IAA	1.0	I
	IAA	2.0	J
	IBA	1.0	K
	IBA	2.0	L