In this work evaluated the effect of size and position of explant in plant and volume different culture medium on the multiplication of Psychotria ipecacuanha (Brot.) Stokes "in vitro" conditions. Experiment with different size and positions was conducted in test tube of 25x150 mm, content 20 mL of culture medium with 0,5; 1,0 and 1,5 cm of size and three different positions (basal, median and apical). In the second experiment were utilized differents volumes (10, 20, 30 and 40 mL) of culture medium. Culture medium utilized on two experiments was MS (MURASHIGE and SKOOG, 1962) supplemented with 1,5 mgL-1 of BAP (6-benzilaminopurine). The differents position not observed influenced, where size of 1,0 and 1,5 showed higher numbers of shoots segments. The different volumes of culture medium were observed that 30 and 40 mL showed higher shoot number.
Psychotria ipecacuanha; medicinal plant; internodal segment
