Saladette-type dwarf tomato introgression lines with agronomic potential, improved fruit quality, and biotic stress tolerance

Oliveira, Camila Soares de; Maciel, Gabriel Mascarenhas; Siquieroli, Ana Carolina Silva; Pinto, Frederico Garcia; Ikehara, Brena Rodrigues Mota; Pereira, Lucas Medeiros

doi:10.1590/1413-7054202448002624

ABSTRACT

Obtaining introgression lines of saladete-type dwarf tomato plants can provide several advantages in breeding programs. In addition to increasing productivity, the dwarf plant can produce metabolites that are important to resistance to biotic stress. However, there are no saladette-type dwarf tomato introgression lines. The objective of this study was to evaluate the agronomic potential, fruit quality, and secondary metabolites associated with pest resistance for the development saladette-type dwarf tomato introgression lines. The experiment was conducted with 23 treatments, including the UFU MC TOM 1 donor parent, the UFU TOM 5 recurrent parent, the Pizzadoro commercial cultivar (control), 5 populations from the first backcross, and 15 populations from the second backcross. Agronomic and nutraceutical characteristics of fruits and the acylsugar content in the leaflets were evaluated. The genetic dissimilarity was calculated using the generalized Mahalanobis distance (D²). Genetic gain through selection was estimated using the rank sum index and the genotype-ideotype distance index. The selection indices showed the importance of obtaining the second backcross. The populations UFU_13_1, UFU_17_1, UFU_10_1, UFUi_11_3, UFUi_10_3, and UFU_11_2 have the potential to obtain introgression lines as they present good agronomic and fruit quality characteristics and acylsugar content similar to UFU MC TOM 1. The dwarf tomato germplasm obtained has significant genetic variability and a saladette-type genetic background with the potential to develop introgression lines. The cultivar UFU MC TOM 1 is promising and can overcome the wild access Solanum pennellii for breeding programs aimed at pest resistance, increasing productivity, and biofortification of fruits to enhance carotenoids.

Index terms:
Solanum lycopersicum L.; dwarfism in vegetables; hybrids; metabolomics; sustainable production.

[1] ^*Corresponding author: gabrielmaciel@ufu.br

Populations	Backcrosses	WM (g)	FL (cm)	FD (cm)	FS	PT (cm)	NL (locules per fruit)	PH (cm)	IL (cm)
UFU _17_1	BC₂	24.53 c ^*	5.01 b ^*	2.84 c ^*	1.77 a ^ns	0.53 b ^*	2.63 a ^*	43.56 b ^*	1.95 c ^*
UFU _10_1	BC₂	24.38 c ^*	4.66 b ^*	2.79 c ^*	1.70 b ^ns	0.51 b ^*	2.94 a ^*	34.38 c ^ns	1.68 d ^ns
UFU_ 4_1	BC₂	24.57 c ^*	4.57 b ^*	2.78 c ^*	1.64 b ^ns	0.45 b ^*	2.64 a ^*	35.90 c ^ns	1.93 c ^*
UFU _11_1	BC₂	20.40 c ^*	4.43 b ^*	2.62 c ^*	1.70 b ^ns	0.48 b ^*	2.73 a ^*	36.56 c ^ns	1.86 c ^ns
UFU _10_2	BC₂	26.58 c ^*	4.87 b ^*	2.59 c ^*	1.88 a ^ns	0.40 b ^*	2.24 b ^ns	37.63 c ^ns	1.78 d ^ns
UFU _4_2	BC₂	23.65 c ^*	5.04 b ^*	2.98 c ^*	1.72 b ^ns	0.49 b ^*	2.54 a ^*	46.50 b ^*	2.26 c ^*
UFU _10_3	BC₂	23.57 c ^*	4.66 b ^*	3.02 c ^*	1.55 b ^*	0.52 b ^*	2.80 a ^*	37.38 c ^ns	1.70 d ^ns
UFU _17_2	BC₂	23.90 c ^*	4.49 b ^*	2.74 c ^*	1.64 b ^ns	0.47 b ^*	2.46 a ^*	39.41 c ^ns	1.92 c ^*
UFU _17_3	BC₂	25.61 c ^*	4.86 b ^*	2.73 c ^*	1.79 a ^ns	0.51 b ^*	2.52 a ^*	46.00 b ^*	1.86 c ^ns
UFU _13_1	BC₂	23.78 c ^*	4.96 b ^*	2.78 c ^*	1.79 a ^ns	0.51 b ^*	2.62 a ^*	41.56 b ^*	1.88 c ^ns
UFU _13_2	BC₂	20.00 c ^*	4.72 b ^*	2.88 c ^*	1.64 b ^ns	0.54 b ^*	2.65 a ^*	35.94 c ^ns	1.61 d ^ns
UFU _11_2	BC₂	20.27 c ^*	4.36 b ^*	2.43 c ^*	1.80 a ^ns	0.48 b ^*	2.63 a ^*	37.31 c ^ns	1.75 d ^ns
UFU _4_3	BC₂	26.61 c ^*	4.86 b ^*	2.92 c ^*	1.69 b ^ns	0.51 b ^*	2.73 a ^*	39.88 c ^ns	1.96 c ^*
UFU _10_4	BC₂	22.12 c ^*	4.51 b ^*	2.54 c ^*	1.78 a ^ns	0.48 b ^*	2.55 a ^*	49.63 b ^*	2.17 c ^*
UFU _11_3	BC₂	24.14 c ^*	5.15 b ^*	2.56 c ^*	2.01 a ^ns	0.48 b ^*	2.86 a ^*	40.28 c ^*	1.97 c ^*
UFU _17	BC₁	22.42 c ^*	4.83 b ^*	2.71 c ^*	1.79 a ^ns	0.53 b ^*	2.55 a ^*	39.31 c ^ns	1.93 c ^*
UFU _4	BC₁	15.24 d ^*	3.95 c ^ns	2.52 c ^*	1.58 b ^*	0.42 b ^*	2.35 b ^ns	32.25 c ^ns	1.57 d ^ns
UFU _13	BC₁	22.67 c ^*	4.74 b ^*	3.20 b ^*	1.50 b ^*	0.50 b ^*	2.79 a ^*	38.63 c ^ns	1.58 d ^ns
UFU _10	BC₁	16.60 d ^*	4.58 b ^*	2.58 c ^*	1.78 a ^ns	0.42 b ^*	2.56 a ^*	43.88 b ^*	1.69 d ^ns
UFU _11	BC₁	16.18 d ^*	3.57c ^ns	2.18d ^ns	1.64 b ^ns	0.33 c ^*	2.25 b ^ns	42.75 b ^*	1.60 d ^ns
Pizzadoro	Check	56.94 b ^*	6.93 a ^*	3.87 a ^*	1.79 a ^ns	0.86 a ^*	2.75 a ^*	94.38 a ^*	7.19 b ^*
UFU TOM 5	RP	63.41 a ^*	6.59 a ^*	3.83 a ^*	1.72 b ^ns	0.85 a ^*	2.78 a ^*	95.38 a ^*	7.86 a ^*
UFU MC TOM1^a	DP	4.62 e	3.19 c	1.65 e	1.94 a	0.20 d	2.00 b	30.25 c	1.22 d
DMS Dunnett		6.91	0.91	0.65	0.32	0.13	0.54	10.11	0.66
Mean		24.88	4.76	2.77	1.73	0.50	2.58	44.31	2.29
CV%		13.12	9.01	11.11	8.63	12.40	9.76	10.77	13.64

Populations	Backcrosses	SSC (°Brix)	LC (mg/100 g)	BC (mg/100 g)	AA (nmol/cm² of leaf area)
UFU_SDI_17_1	BC₂	5.36 b^*	3.03 b ^ns	3.01 a ^ns	22.43 c ^ns
UFU_SDI_10_1	BC₂	5.54 b^*	2.99 b ^ns	2.50 a ^ns	27.69 b ^ns
UFU_SDI_4_1	BC₂	5.64 b^*	2.29 c ^ns	2.31 b ^ns	27.37 b ^ns
UFU_SDI_11_1	BC₂	5.25 b^*	2.93 b ^ns	2.72 a ^ns	30.02 b ^ns
UFU_SDI_10_2	BC₂	5.77 b^*	2.74 c ^ns	2.44 a ^ns	27.24 b ^ns
UFU_SDI_4_2	BC₂	5.69 b ^*	2.73 c ^ns	2.09 b ^ns	23.17 c ^ns
UFU_SDI_10_3	BC₂	5.54 b^*	3.02 b ^ns	2.56 a ^ns	27.39 b ^ns
UFU_SDI_17_2	BC₂	5.55 b^*	3.57 a^*	3.47 a ^ns	19.71d ^*
UFU_SDI_17_3	BC₂	5.43 b^*	2.77 c ^ns	1.64 b^*	20.74 c ^*
UFU_SDI_13_1	BC₂	5.75 b^*	2.95 b ^ns	2.92 a ^ns	27.59 b ^ns
UFU_SDI_13_2	BC₂	5.29 b^*	2.77 c ^ns	2.45 a ^ns	23.84 c ^ns
UFU_SDI_11_2	BC₂	5.90 b^*	3.03 b ^ns	2.83 a ^ns	27.99 b ^ns
UFU_SDI_4_3	BC₂	6.10 b^*	2.52 c ^ns	2.30 b ^ns	24.61 b ^ns
UFU_SDI_10_4	BC₂	5.38 b^*	2.81 c ^ns	2.46 a ^ns	29.42 b ^ns
UFU_SDI_11_3	BC₂	6.00 b^*	3.12 b ^ns	2.25 b ^ns	20.97 c ^*
UFU_SDI_17	BC₁	5.67 b^*	2.53 c ^ns	2.59 a ^ns	18.24 d ^*
UFU_SDI_4	BC₁	6.54 b^*	2.34 c ^ns	1.73 b^*	23.65 c ^ns
UFU_SDI_13	BC₁	5.53 b^*	2.42 c ^ns	1.94 b ^ns	27.29 b ^ns
UFU_SDI_10	BC₁	5.66 b^*	2.56 c ^ns	2.61 a ^ns	27.51 b ^ns
UFU_SDI_11	BC₁	4.91 b ^*	3.78 a^*	3.17 a ^ns	18.42 d ^*
Pizzadoro	Check	4.81 b^*	2.23 c ^ns	2.57 a ^ns	15.48 d ^*
UFU TOM 5	RP	5.07 b^*	2.64 c ^ns	1.66 b ^ns	16.57 d ^*
UFU MC TOM 1^a	DP	8.71a	2.50 c	2.78 a	29.18 b
Solanum pennellii	Wild access	-	-	-	37.16 a^*
DMS Dunnett		1.33	0.67	0.99	6.68
Mean		5.69	2.79	2.47	24.73
CV%		11.02	11.33	18.83	12.76

Variables	%SG
Variables	MM	GIDI
WM	9.95	8.33
FL	4.27	3.31
FD	2.78	2.05
FS	1.07	0.79
PT	7.21	6.21
NL	4.69	3.62
PH	-0.55	-0.67
IL	1.24	1.06
SS	-0.41	-1.53
LC	3.57	7.87
BC	3.19	7.98
AA	1.79	-0.49
Total	38.8	38.5
Selected populations	UFU_13_1	UFU _13_1
	UFU_17_1	UFU _17_1
	UFUi_11_3	UFU _11_2
	UFU_4_3	UFU _11_3
	UFU_10_1	UFU _10_3
	UFUi_10_3	UFU _10_1
	UFU_11_2	UFU _17_2

Brasil

Brasil

Saladette-type dwarf tomato introgression lines with agronomic potential, improved fruit quality, and biotic stress tolerance

Linhagens de introgressão de tomateiro anão do tipo saladete com potencial agronômico, qualidade de frutos e tolerante a estresse biótico

ABSTRACT