The influence of various growth regulators and medium concentrations, in different quantities, on the in vitro callus induction of carqueja [Baccharis trimera (Less.) DC.] was evaluated. It was found that the callus initiation was dependent on both, the growth regulator and medium concentration. The highest callus induction and development were obtained by using 15.0 mM 1-napthaleneacetic acid (NAA) as growth regulators and half strength of salts, vitamins, and myo-inositol of Murashige and Skoog medium. In vitro shoot proliferation was obtained by using thidiazuron.
Baccharis trimera; medicinal plant; auxin; cytokinin
