Identification of a novel germplasm (Jor Lab L-9) of lemon grass(<i>Cymbopogon khasianus</i>)rich in methyl eugenol

Lal, Mohan; Munda, Sunita; Dutta, Sukriti; Pandey, Sudin Kumar

doi:10.1590/1984-70332020v20n3c49

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Crop Breeding and Applied Biotechnology

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CULTIVAR RELEASE • Crop Breed. Appl. Biotechnol. 20 (3) • Jul-Sep 2020 • https://doi.org/10.1590/1984-70332020v20n3c49 copy

Identification of a novel germplasm (Jor Lab L-9) of lemon grass(Cymbopogon khasianus)rich in methyl eugenol

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

A new strain of Cymbopogon khasianus rich in methyl eugenol was identified and named Jor Lab L-9. It is stable for production of essential oil yield (average 0.81%; $σ_{d i}^{2} = 0.00$ ) and methyl eugenol (average 74.56%; $σ_{d i}^{2} = 0.06$ ). This variety was registered with ICAR-NBPGR, New Delhi, India, under registration number INGR-18037.

Keywords:
Cymbopogon khasianus; essential oil quality; methyleugenol

Sl.No.	Trait	Range
1	Vegetative plant height (cm)	69-143
2	Flowering plant height (cm)	91-250
3	Number of tillers bush^-1	87-92
4	Number of leaves	485-526
5	Length of lamina (cm)	56-73
6	Breadth of leaf (cm)	0.4-0.8
7	Length of basal sheath (cm)	12-34
8	Length of ligule (cm)	0.1-0.5
9	Length of auricle (cm)	0.09-0.1
10	Colour of leaf sheath	Brownish green
11	Number of nodes	4-12
12	Number of internodes	3-11
13	Diameter of nodes (cm)	1-2.20
14	Diameter of internodes (cm)	0.50-1.20
15	Length of internodes (cm)	16-27.70
16	Length of spike (cm)	78.20-110.50
17	Number of spikelets	7-12
18	Length of awn (cm)	0.70-1.10
19	Flowering time	last week of October to December
20	Herbage Yield (tones^-1 ha^-1 year^-1)	18.3 to 36.4
21	Essential oil (%)	0.5-0.85
22	Methyl eugenol (%)	2-77

Sl.No.	Trait	Quantitative data
1	Vegetative plant height (cm)	109.83±1.81
2	Flowering plant height (cm)	230.28±4.40
3	Number of tillers bush^-1	95.53±3.38
4	Number of nodes	13.20±0.66
5	No. of internodes	12.17±0.82
6	Herbage Yield (tons ha^-1 year^-1 )	29.50±0.90
7	Essential oil (%)	0.81±0.01
8	Methyl eugenol (%)	74.65±0.19
9	Flowering time	October to December

S. No.	Compound	Retention time	Area (%)	RI^a	RI^b	Identification method
1	α-pinene	6.802	0.29	912	917	1,2,3
2	Camphene	7.179	0.70	923	933	1,2,3
3	β-pinene	7.677	1.11	957	964	1,2,3
4	β-myrcene	9.222	14.28	976	981	1,2,3
5	α-terpinene	10.676	0.61	1011	1016	1,2
6	Limonene	11.065	0.88	1022	1031	1,2,3
7	1, 8 cineole	11.494	0.54	1032	1036	1,2,3
8	Trans-β-ocimene	13.21	0.05	1043	1050	1,2
9	α-terpinolene	13.714	0.36	1083	1097	1,2
10	Linalool	16.621	0.06	1099	1100	1,2,3
11	Borneol	17.765	0.06	1161	1165	1,2,3
12	α-terpineol	18.114	0.09	1182	1190	1,2
13	Nerol	20.643	0.11	1223	1229	1,2,3
14	Bornyl acetate	22.04	0.42	1282	1286	1,2
15	α-cubenene	25.45	0.05	1340	1345	1,2
16	α-copaene	26.302	0.05	1371	1378	1,2
17	Methyl eugenol	27.367	74.23	1392	1402	1,2,3
18	Cis- α-bergamotene	27.762	1.19	1428	1436	1,2
19	β-caryophyllene	28.454	0.19	1446	1451	1,2,3
20	α-humulene	29.169	0.15	1452	1456	1,2
21	γ-Muurolene	30.949	0.18	1472	1478	1,2
22	Elemicin	31.67	1.65	1561	1566	1,2,3
23	Caryophyllene oxide	31.984	0.36	1583	1589	1,2,3
24	Epicubenol	33.306	0.10	1622	1627	1,2
25	Cadinol	37.146	0.89	1672	1679	1,2
Total identified (%)		98.60
Monoterpenes (%)		19.56
Sesquiterpenes (%)		3.16
Phenylpropanoid (%)		75.88

Sl. No.	Variety Location		Vegetative plant height (cm)	Flowering plant height (cm)	Number of tillers bush^-1	Number of nodes	Number of internodes	Herbage Yield (tonnes ha^-1 year^-1)	Oil % (v/w)	Methyl eugenol (%)	Major oil constituent
1	Jor Lab L-9	Jorhat	106.81	236.27	92.33	12.07	11.13	29.86	0.81	74.98	Methyl eugenol
		Imphal	108.66	219.55	95.60	12.47	11.33	28.78	0.81	72.77
		Pasighat	111.56	238.17	95.13	14.67	13.73	29.57	0.82	74.60
		Lakhmijan	112.29	227.12	99.07	13.60	12.47	29.81	0.81	75.90
		Avg	109.83	230.28	95.53	13.20	12.16	29.51	0.81	74.56
2	RRLJM-637 (IC0626782)	Jorhat	104.17	177.65	55.13	11.73	11.07	22.16	0.38	25.75	Methyl eugenol
		Imphal	103.05	189.09	59.87	12.00	10.93	21.59	0.38	25.99
		Pasighat	105.94	192.92	58.40	14.20	13.20	21.55	0.39	25.87
		Lakhmijan	109.87	202.55	61.00	12.73	11.73	21.99	0.39	25.40
		Avg	105.76	190.55	58.60	12.66	11.73	21.83	0.39	25.76
3	Jor Lab L 2	Jorhat	106.04	182.55	52.40	12.40	11.33	21.40	0.37	4.19	Citral
		Imphal	110.72	192.03	55.20	13.60	13.13	21.81	0.43	3.48
		Pasighat	114.32	190.43	61.00	12.87	12.40	20.70	0.40	3.42
		Lakhmijan	113.81	192.89	55.20	12.40	11.87	20.87	0.40	3.83
		Avg	111.22	189.48	55.95	12.81	12.18	21.20	0.40	3.73
		SE (m)	1.64	13.43	12.77	0.16	0.15	2.67	0.14	20.93
		CV	2.60	11.43	31.60	2.16	2.11	19.13	44.93	104.51

	Vegetative plant height			Herbage yield			Essential oil yield			Methyl eugenol (%)			Flowering plant height
Genotypes	μ	βi	$σ_{d i}^{2}$	μ	βi	$σ_{d i}^{2}$	μ	βi	$σ_{d i}^{2}$	μ	βi	$σ_{d i}^{2}$	μ	βi	$σ_{d i}^{2}$
Jor Lab L-9	109.8	0.57	5.82**	29.51	1.43	0.41	0.81	0.57	0.00	74.56	1.13	0.06	230.3	-0.12**	107.15***
RRLJM-637	105.8	1.19	16.57***	21.83	0.93	0.42	0.39	0.33	0.00	25.76	1.17	1.43***	190.6	0.89	279.89***
Jor Lab L 2	111.2	1.23	29.97***	21.20	0.64	0.23	0.40	2.10	0.00	3.73	0.22*	-0.01	189.5	2.23*	267.14***
Population mean	108.9			24.18			0.53			34.68			203.4

	Number of tillers bush^-1			Number of nodes			Number of internodes
Genotypes	μ	βi	$σ_{d i}^{2}$	μ	βi	$σ_{d i}^{2}$	μ	βi	$σ_{d i}^{2}$
Jor Lab L-9	95.53	0.44	21.36 ***	13.20	1.54	0.19	12.16	1.50	0.46
RRLJM637	58.60	0.70	26.09 ***	12.66	1.07	0.05	11.73	1.08	0.02
Jor Lab L 2	55.95	1.85	26.50***	12.81	0.39	0.29	12.18	0.41	0.64*
Population mean	70.03			12.89			12.02

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