Concentration and gene expression analyses of dragon′s blood flavonoids in different tissues of Dracaena cochinchinensi

ABSTRACT

Background:

Dragon′s blood is a well-known traditional Chinese medicine, isolated mainly from the trunks of Dracaena trees. Overexploitation of the Dracaena resource has resulted in this plant becoming endangered. This work aimed to assess the potential to use various tissues of the Dracaena cochinchinensis tree, such as leaves and roots, to produce dragon′s blood.

Results:

We found that many dragon′s blood components accumulated in the bark of trunk and roots of D. cochinchinensis under natural conditions. In addition, small amounts of loureirin A were detected in leaves, suggesting that the leaves could be a potential source of dragon′s blood. Real-time quantitative PCR analysis showed that most of the genes tested in this study, which encoded enzymes involved in the biosynthesis of dragon′s blood flavonoids, were highly expressed in the bark of roots and trunk.

Conclusion:

We confirmed that the bark of roots and trunk of D. cochinchinensis tree were the main tissues for the synthesis and storage of dragon′s blood under natural conditions. This study demonstrated the potential to extract dragon′s blood from the roots that have been abandoned due to mining difficulties without destroying the tree, a process which would be beneficial to the protection of the endangered wild D. cochinchinensis tree populations.

Keywords:
Dracaena tree ; Dragon′s blood accumulation site; Gene expression; Loureirin A; Loureirin B

HIGHLIGHTS

The bark of trunk and roots are the main tissues for the synthesis and storage of dragon’s blood in Dracaena tree.

The roots of Dracaena tree can be used to extract the dragon’s blood.

The leaves of the Dracaena tree have the potential to produce dragon’s blood.

The production of the red substance can be used as a marker of dragon’s blood synthesis in Dracaena tree.