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ABSTRACT Background: Today, the biggest threat to mammalian predators with wide distribution areas is habitats fragmentation or changing climate conditions. We aimed to reveal the habitat suitability modeling and mapping of the Brown bear, which is an important large mammal in Turkey’s borders, under change climate. The habitat suitability modelling was determined using the present (2010) and future (2040-2070-2100) chelsa climate scenarios (IPSL-CM6A-LR SSP126-SSP370-SSP585) Maxent method with the present data obtained by examining all studies on Brown bear. Then, the mapping result values for the different years and scenarios were classified as 0.5 unsuitable habitats, 0.51-0.8 suitable habitats and 0.81-1.0 most suitable habitats. Results: We determined that the variables contributing to the habitat suitability model of Brown bear are annual precipitation amount, the average annual air temperature, the precipitation amount of the wettest month, the ruggedness and elevation. According to the mapping results for different years and scenarios; Brown bear have suitable habitat a minimum of 14.87% of the study area in today, 12.56% in 2040 year, 10.93% in 2070 year and 8.24% in 2100 year. According to the SSP585 climate scenario of 2100 year, the habitat suitability of the Brown bear decreases by approximately 45%. Also, the climate envelope model created with MaxEnt revealed, the change climate in the 2100 year endangered the Brown bear. Conclusion: Therefore, these results will be a source of information for the sustainability of the extinction of the Brown bear, for the pre-protection of existing and potential habitats and for reducing the impact of change climate conditions.

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ABSTRACT Background: Climate change, which has mainly manifested in Iran in the form of intensifying periods of drought, can have profound effects on the valuable forest ecosystems of Zagros in the west of Iran. In this study, the drought trend in the period from 2000 to 2020 was investigated on the spatio-temporal dynamics of greenness of Zagros oak forests in Kohgiluyeh and Boyer Ahmad province in the west of Iran. SPI, PDSI, NDVI and EVI with modeling their relationship based on GWR was used. Also, based on two climate change scenarios RCP2.6 and RCP8.5, the simulation of drought conditions and changes in forest greenness until 2050 were studied. Results: The results showed that the peak greenness of the oak forests has a significant sensitivity to changes in rainfall and drought at the end of the cold period of the year and in the spring season. The negative phases of the drought have been completely consistent with the periods of greenness loss. Also, both the greenness and the area of ​​the oak forests have decreased. The EVI index showed the highest sensitivity to the PDSI, and the developed model based on these two indices had a spatial explanation coefficient between 40 and 70%. Conclusion: The implementation of the developed model under two scenarios showed that the forest greenness will face a decrease of about 25% in the RCP8.5 scenario and about 15% in the RCP2.6 scenario until 2050. The relationship between drought and forest decline was proven in this study.

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ABSTRACT Background: Bamboo species have a high potential to produce biomass and stock carbon. However, biometric tools are not available to estimate biomass production for most giant bamboo taxa. The aim was to develop an additive equation system to estimate the aboveground biomass by structural component and the total of the three bamboo species. Destructive sampling was applied, and a sample of 101 mature bamboo specimens was collected. The nonlinear power allometric model was used to integrate two additive equations systems, which were formed by the structural components of biomass: culm, branches and leaves as well as aboveground biomass total. The predictor variables were: diameter at breast height (D) for the S1 system, and D in combination with the total height (D2H) for the S2 system. Results: It was determined that the SUR method in combination with the dummy variables technique and the correction of heteroscedasticity is an adequate fit strategy. Given that the additivity property is fulfilled, specific values of the parameters of each system and by taxon are identified. In addition, the variance of the error stabilizes. The aboveground biomass of the culm constitutes 86.40%, 90.48%, and 93.94% for Bambusa oldhamii Munro, Guadua aculeata Rupr., and Guadua angustifolia Kunth, respectively. The S1 system was selected, and its statistics regarding the total aboveground biomass were 0.92, 4.9 kg, -0.35 kg, and 0.05 for the fit statistics R 2 adj , RMSE, S, and E, respectively. Conclusion: This biometric tool will easier to carry out aboveground biomass inventories, as well as to infer the carbon content and CO2 equivalent at the specimen level.

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ABSTRACT Background: Vegetation indices have recently been proposed for remote sensing SAR (synthetic aperture radar) sensor measurements to monitor vegetation. However, they still lack validation studies on different vegetation types for their correct application. Thus, the objective of this study was to test the applicability of the Dual-polarization SAR Vegetation Index (DPSVI) and the modified DPSVI (DPSVIm) as indicators of aboveground biomass (AGB) from dense forest fragments. Results: Three forest fragments, comprising 54 forest plots with AGB ranging from 12 up to 220 Mg ha-1, were studied. These forest fragments belong to the Brazilian Atlantic Forest biome, and were located within the Doce river hydrographic basin in the state of Minas Gerais, Brazil. AGB was compared with the DPSVI and DPSVIm indices, computed from dual-polarization Sentinel-1 images, using Spearman’s rank correlation test through two approaches. In the first approach (A1), correlation tests were performed using all forest plots; in the second approach (A2), samples were taken from plots on flat to undulating terrain slopes. The correlation of AGB with DPSVI presented no significant correlation (p-value >> 0.05). In contrast, for DPSVIm, the correlation with AGB was significant and positive, with coefficients ranging from 0.4 in approach A1 to 0.7 in approach A2. Conclusion: While the DPSVI index did not show a correlation with the AGB of the studied forests, even though it is a C-band index, the DPSVIm was found to be a good indicator of the amount of AGB in forests and therefore has potential for applications in future studies, particularly in areas with reasonable slope or flat terrain.

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ABSTRACT Background: The objective of this study was to detect native trees from different flight configurations and smoothing techniques in Canopy Height Models (CHMs) in a native remnant in the municipality of Curitiba, State of Paraná, Brazil. To do so, eight flights were carried out with a Phantom 4, with two flight planning applications (Litchi and Pix4Dcapture) and two flight arrangements (single and double), totaling four flights for each application. All flights were processed using the Pix4Dmapper program. The LiDAR database was obtained with a DJI Matrice 300 system and from this data, the Digital Terrain Model (DTM) of the area was extracted. From the UAV data, the Digital Surface Model (DSM) of each flight was obtained. Subtracting each DSM from the DTM resulted in the CHMs for each UAV flight flown. The CHMs were smoothed with the CHMsmoothing function and three search window sizes were tested (6.5 x 6.5, 8 x 8, and 10 x 10). Results: The results of the ITD approach revealed that in unsmoothed and smoothed CHMs, the search window of size 8 resulted in the best precision metrics, with the highest recall, precision, and F-score values. In the smallest window size, there was the highest number of false positives while in the largest window size, the omitted trees were more representative. Conclusion: The best combination between flight parameters and smoothing techniques was with the Litchi application, with a single flight and 80% lateral and longitudinal overlap, resulting in individuals detected with an F-score of 0.94.

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ABSTRACT Background: Hancornia speciosa is a species known for its medicinal and nutritional properties. Despite being a plant native to several regions of Brazil, some aspects of its production system are not yet well defined. Large-scale asexual propagation stands out, making it difficult to establish commercial orchards with materials with superior characteristics. In this context, this article aimed to investigate the scientific production related to the propagation and conservation of the mangabeira, using a mixed methodology of bibliometric analysis and literature review. The Scopus and Web of Science databases were consulted and the VOSviewer software was used to map the cooperation network between authors, institutions, and keywords. Results: The study included 72 articles and found a significant increase in scientific production on mangabeira propagation and conservation since 2009. Most studies focused on seed technology, seedling production, in vitro propagation and conservation, and cryopreservation. Additionally, it was observed that mangabeira seeds do not tolerate a reduction in moisture content, and the combination of materials for the composition of the substrate, such as coconut fiber, manure, and sand, can provide an adequate basis for developing seedlings. It was also observed that MS culture medium, complete or with half saline concentration, or WPM medium can be used for in vitro germination and initial seedling growth. Conclusion: Through this review, we concluded that the mangabeira presents substantial potential for the development of innovative propagation techniques, with a special focus on methods that facilitate the clonal propagation of the species, such as grafting, rooting cuttings, and micropropagation. It is expected that future research will expand knowledge about the in vitro and ex vitro propagation of this species, contributing to the expansion of commercial plantations with a focus on adding to the processing of its fruits.

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ABSTRACT Background: The objective of this study is to characterize the diversity and genetic structure of A. colubrina in three Atlantic Forest fragments in the south of Espírito Santo state, using Inter Simple Sequence Repeats (ISSR) molecular markers. Genomic DNA from 85 trees was analyzed using 12 ISSR primers, generating 147 fragments, of which 109 were polymorphic (74%). Markers were characterized as moderately informative, with a mean polymorphic informational content of 0.34. Results: Low genetic diversity was found for the three fragments and in the total sample, based on Nei’s diversity parameters (H* = 0.26) and Shannon’s index (I* = 0.38). In the dissimilarity analysis, four groups were observed in the dendrogram with an exclusive clustering trend by location. The analysis of molecular variance confirmed that most of the genetic variation is found within populations (73.50%), however, with high genetic differentiation between them (Φ ST = 0.26). This result was supported by the Bayesian approach that indicated genetically structured populations. Conclusion: The data obtained reveal that forest fragmentation affected the diversity and genetic structure of A. colubrina and allow expanding knowledge for the development of effective strategies for the conservation and management of the species.

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ABSTRACT Background: The objective of this study was to evaluate the quality indicators for wood and charcoal from the Eucalyptus urophylla x Eucalyptus grandis hybrid (Clone GG 100) in different planting spacings. The study was conducted with the hybrid using the spacings of 2×1, 2×2, 3×2, and 3×3 m. The chemical properties of wood and charcoal were analyzed, and dry wood mass and energy per hectare were estimated. The data were subjected to the t test for comparison of means. Moreover, principal component analysis was conducted to assess the relationships between wood and charcoal indicators as a function of spacing. Results: The 2×2 m spacing showed the highest values for dry wood mass, lignin mass, mass of charcoal, and energy. The wider spacings of 3×2 and 3×3 m resulted in wood and charcoal of increased quality by showing wood of greater resistance to thermal degradation and charcoal of higher yield. Conclusion: Spacing between trees influenced wood quality indicators. The 2×2 m spacing showed higher estimates of mass and energy per hectare. However, wide spacings with an area greater than 6 m2 are more suitable for charcoal production due to their superior wood properties, especially basic density and total lignin, which influence gravimetric yields and bulk density.

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ABSTRACT Backgrounds: Urban and peri-urban fragments are vital for biodiversity conservation, requiring genetic assessment of tree species in fragmented forests. The aim of this study was to analyze the genetic variability and diversity for adult individuals of J. micrantha along an urban-rural gradient in the Araucaria Forest. Fifteen individuals were sampled, with five from each remaining forest type. Initially, 10 ISRR primers were tested. Five mother trees were chosen from each site (urban, peri-urban, and rural) with a minimum distance of 100 m. The experimental design was a RCBD with 15 progenies, three provenances, three blocks, and 20 plants per plot, totaling 900 seedlings. Results: The average percentage of polymorphic loci was 93.33%. The urban population showed a greater loss of genetic diversity (H=0.1806). 79% of the genetic diversity was found within populations. The observed gene flow value (Nm) was 1.8790, indicating that there were no random losses of alleles within populations. The fragments did not exhibit significant differences, but there were significant differences among the progenies. The stem diameter (SD) and the height-diameter relationship (H/SD) emerged as the key traits for selecting new individuals due to their higher heritability (< 0.50), accuracy (< 0.70), and relative coefficient of variation (< 7%). Conclusion: The urban fragment is the most affected, but gene flow between fragments prevents the random loss of alleles. The analysis suggests that these fragments form a unique population, despite geographic barriers. Thus, the three fragments can be considered when choosing superior individuals for future progeny tests in genetic improvement programs for the species.

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ABSTRACT Background: The restricted dispersibility of cellulose in water has grown to be a significant problem which is a key step in making cellulose soluble in water and common solvent. To overcome the obstacle, cellulose structure is being modified to improve the surface properties and the utilization of the cellulose itself. In this study, cellulose I and cellulose II were examined after treated with water-soluble 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO). The hydrogen bond between chains and the crystallinity of the cotton linter (cellulose I) was first broken by NaOH/urea. Then, cellulose I and NaOH/urea-treated cellulose (cellulose II) were oxidized with sodium chlorite, sodium bromide, and TEMPO in a catalytic amount. Results: The success of oxidation is achieved when both cellulose I and II treated with TEMPO had preferentially converted the hydroxyl groups to carboxylate groups through Fourier Transform Infrared Spectrometer (FTIR). Besides, through X-ray diffraction analysis, cellulose I and II exhibited a decreased in the crystallinity. The scattered structure revealed through morphology analysis proved that cellulose treated with TEMPO had resulted in a more dispersed and separated cellulose fibre structure. Conclusion: The result showed, cellulose has been successfully modified using TEMPO-mediated oxidation with improved dispersion properties. The scattered structure revealed through morphology analysis proved that cellulose treated with TEMPO had resulted in a more dispersed and separated cellulose fibre structure. High cellulose dispersion ability will allow the manufacturing process of hydrogel, film and fibre to be much easier and faster. This is necessary for creating novel, environmentally friendly materials for various applications across numerous industries and future research anticipated to increase.

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ABSTRACT Background: The macauba (Acrocomia aculeata (Jacq.) Lodd. ex Mart.) is a palm tree native to tropical regions and occurs in much of the Brazilian territory. Current studies are focused on the extraction and exploitation of vegetable oil from this fruit. However, there are few studies on the residue, the endocarp, which represents approximately 25% of the total mass of the fruit. Studies that search for solutions for using residue, thus adding value, and generating environmentally friendly products, are of fundamental importance. Thus, this research aimed to evaluate the physical and mechanical properties of reconstituted wood panels produced with different proportions of macauba endocarp particles to replace pine wood particles, in addition to characterize the physical and chemical properties of macauba endocarp. The chemical characterization tests were performed according to applicable standards. The basic density was determined according to the NBR 11941 standard. Thermogravimetric analysis (TGA), X-ray diffractometry (XRD) and Fourier transform infrared spectroscopy (FTIR) were also performed. The particleboards panels were produced with a nominal density of 0.70g/cm³ and dimensions of 25x25x1.5cm. Five different treatments were evaluated with variations in the proportion of macauba endocarp particles in the core of the panel. For particle agglutination, phenol-formaldehyde adhesive was used at a proportion of 9% for the core and 12% for the faces, based on the dry mass of each panel. Scanning electron microscopy (SEM) was used to analyze the panel surfaces. Results: The endocarp of Acrocomia aculeata has low extractive content, high lignin content and high densification, 1.23g/cm³, when compared to Pinus oocarpa wood, 0.43g/cm³. All panels were classified as low density according to CS 236-66 and had an average apparent density between treatments of 0.586g/cm³. A greater dimensional stability and a decrease in the static bending properties of the panels was observed as the proportion of macauba endocarp particles increased. The internal bonding of the panels showed a positive result with the increase in the use of particles of the residue. Conclusion: The panels produced have potential for use for non-structural purposes.

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ABSTRACT Background: Paulownia tomentosa wood has chemical properties that satisfy the requirements for good raw material to obtain cellulose-based products, such as nanocellulose. Cellulose nanocrystals (CNC) are derived from naturally occurring cellulosic fibers, constituted of cellulose chains with an organizational setting that results in rigid rod-shaped crystals. This study assessed the wood wastes from Paulownia tomentosa Steud. as raw material for producing cellulose nanocrystals (CNC) using alkaline and delignification treatments, followed by a hydrolysis process with sulfuric acid at the 52% and 58% concentrations. The isolation of CNC from Paulownia tomentosa wood wastes was confirmed through different spectroscopic analyses. Results: The suspensions of nanocrystalline cellulose CNC-52 and CNC-58 showed yields of 8.34% and 7.62%, respectively. The particle size distribution of the suspensions, determined by the AFM technique, presented an average of L = 180.01 nm and W = 20.46 nm in CNC-52 and L = 128.06 nm and W = 10.18 nm in CNC-58. Moreover, the FTIR and XRD results demonstrated that there was no difference in the structure of the crystalline network and the chemical composition between cellulose (Kiri-CB) and the CNC. Conclusion: The results obtained from this study allowed to conclude that it is possible to employ the Paulownia tomentosa wood waste as a source of cellulose for extracting CNC by hydrolysis, adjusting the sulfuric acid concentration to 58% and maintaining it at 45 °C for 60 min.

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ABSTRACT Background: The use of X-rays non-destructive technique in color scale in 2D and 3D needs further studies to understand variations in the apparent density of wood stored under natural conditions. The objective of this study was to evaluate, by digital X-ray images and to test the rainbow scale in digital X-ray images in 2D and 3D, the apparent density of eucalyptus wood after storage in the field during 365 days. Trees of two diametric classes were randomly distributed at three heights in a pile in direct contact with the soil. The apparent density of the wood and its diametric profiles in the radial direction were determined using digital X-ray images in gray scale, and converted to a rainbow scale and the density variations plotted on a 3D surface. Results: The wood density decreased as the storage period increased. The density of the samples at 0 days of storage evaluated with the 3D images was homogeneous, while an intense attack by xylophagous organisms due to the exposure to the environmental conditions was observed in those collected at 365 days of storage. Conclusion: The rainbow scale color from X-ray images facilitated to visualize and to interpret the variation of apparent density distribution of the wood.

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ABSTRACT Background: Converting açaí waste fibers into kraft pulp and paper demands high amounts of preserved fibers. Manual removal preserves the fibers, but mechanical methods are faster. Therefore, this work aimed to compare three methods to extract açaí fibers from the seed’s surface concerning efficiency and fiber quality for cellulose pulping. Results: Açaí waste fibers have ≈ 34 % of cellulose and ≈ 61% of non-cellulose structural components (based on extractive free mass) and ≈ 6% of non-structural extractives and ashes (based on total mass). They occur united into bundles that dissociate into short (388 µm) fiber cells. Their pulp-paper quality indexes were aspect ratio (31.8-41.2), wall fraction (52.8 %), flexibility coefficient (47.2 %), boiler index (0.6), Runkel index (1.2), and Mulsteph index (0.8). The manual removal preserved the fibers but had the lowest efficiency (0.1 g/min). The food processor provided intermediate preservation of the fibers and efficiency (0.5 g/min). Despite the highest efficiency (3.9 g/min) of the hammer mill method, the friction with the hammers damaged the fibers and increased the levels of extractives from 4 to 8% and hemicelluloses from 34 to 40%. Conclusion: Açaí waste fiber bundles are dissociable into short fibers and have a favorable chemistry for kraft pulping and developing cellulose products. The fiber morphology is not ideal, but not limiting, demanding adjustments in the future kraft pulping parameters. The extraction of the fiber by the food processor is recommended, which is manageable to the local communities to support an integrated bioeconomy of the açaí waste.

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ABSTRACT Background: The production of cellulose microfibrils/nanofibrils (CMF/CNF) has attracted increasing attention in recent decades due to their excellent barrier, mechanical, and surface chemistry properties. However, large-scale industrial production of CMF/CNF has been a major challenge due to their high energy consumption, limiting their application. In this context, in recent years many studies have focused on developing pre-treatments designed to facilitate the fibrillation of CMF/CNF by reducing energy consumption during their production. This review highlights the latest advances in the use of silicates as chemical pre-treatments for CMF/CNF production, covering the main aspects related to the effects of chemical modification on the production and the properties of materials for application in biodegradable packaging. Results: Energy consumption reductions of up to 30% were achieved by pretreating cellulose pulps using silicates. In addition, the pre-treatments resulted in smaller CMF/CNF diameters and greater individualization of the nanofibrils. Studies evaluating the thermal stability, hydrophobicity, mechanical properties, and porosity of CMF/CNF pre-treated with silicates have reported promising results. The application of CMF/CNF pretreated with calcium and magnesium silicates in cardboard coating resulted in packaging with low water vapor permeability and high ductility. Conclusion: Silicates interact well with cellulose surfaces, making them a promising material for the chemical pre-treatment of CMF/CNF. Furthermore, the modification by silicates could be an interesting strategy for expanding the use of CMF/CNF in the development of new products.