Abstract
This study had as objectives to determine the best shading condition and the type of substrate to produce seedlings and the establishment of a spontaneous symbiosis by rhizobia in taxi-branco (Tachigali vulgaris). A randomized block design with four replications and treatments arranged in a split-plot scheme was installed. The plot consisted of two shading levels (50% and 75%) and subplots of four types of substrates: soil; soil + sand (1:1); soil + sand + sawdust (2:1:1); and soil + vermiculite (1:1). Each experimental plot consisted of five seedlings. The seedlings were evaluated for a set of shoot and root morphological and physiological traits. At the end of the experiment, the isolation and the phenotypic characterization of bacteria from nodules collected in the seedlings were also carried out. The characterization was performed by the morphological evaluation of the colonies. The shading of 50% is indicated for seedling productions, while in relation to the substrate, that one composed by soil + sand is what produces seedlings of better quality. Although the number of isolates was not influenced by the substrate type and the shading level, taxi-branco seedlings establish symbiosis with a great diversity of rhizobia. Most of the isolates have very fast growth rate and acid pH of the culture medium.
Keywords:
Taxi-branco; Nursery; Biological fixation of nitrogen
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