IN VITRO REGENERATION OF LOURO-PARDO (Cordia trichotoma (Vellozo) Arrabida ex Steudel)

ABSTRACT

This study was carried out aiming at evaluating the in vitro regeneration of louro-pardo (Cordia trichotoma (Vellozo) Arrabida ex Steudel). Nodal segments were desinfested and innoculated in two basal media: WPM (Wood Plant Médium, 1981) and MS (Murashige & Skoog, 1962) supplemented with BAP (6-Benzylaminopurine), TDZ (Thidiazuron), NAA (Naphthaleneacetic Acid), GA₃ (Gibberellic Acid), IBA (Indole-3-Butyric Acid) and A.C. (Activated Charcoal) in different combinations and concentrations for each developmental step. The regeneration rate and the shoot elongation were higher in the WPM medium containing 0,1 mg.l^-1 of BAP and GA₃. The rooting of regenerated shoots was observed in IBA (0,5 mg.l^-1) and Ativated Charcoal (1,5 g.l^-1).

Key words:
Cordia trichotoma; in vitro regeneration; basal media MS; basal media WPM, regulators of growth