Expression and clinical significance of miR-141-5p as a biomarker in the serum of patients with early spontaneous abortion

Highlights

• Correlation analysis of miR-141-5p and serum β-HCG, P4 and E2 levels in patients with ESA.

• miR-141-5p as a diagnostic biomarker for ESA.

• β-HCG, P4 and E2 levels.

Abstract

Aim

miR-141-5p expression in patients with Early Spontaneous Abortion (ESA) and its correlation with hormone levels during pregnancy were investigated.

Methods

A total of 70 pregnant women with ESA were selected as the research group, and 70 normal pregnant women who chose abortion for non-medical reasons were selected as the Con group. Serum β-HCG, Progesterone (P), and Estrogen (E2) were detected by enzyme-linked immunosorbent assay. Differentially expressed miRNAs were screened by miRNA microarray analysis. miR-141-5p expression was detected by RT-qPCR, and its correlation with serum β-HCG, P, and E2 levels was analyzed. The diagnostic value of miR-141-5p for ESA was evaluated by the ROC curve.

Results

Serum β-HCG, P, and E2 were decreased and serum miR-141-5p was increased in patients with ESA. Pearson correlation analysis showed that serum β-HCG, P, and E2 levels were negatively correlated with miR-141-5p expression levels. ROC curve showed that miR-141-5p had a diagnostic value for ESA.

Conclusions

miR-141-5p is related to hormone levels during pregnancy and is expected to become a new candidate diagnostic marker for ESA.

Keywords
Early spontaneous abortion; Progesterone; miR-141-5p; β-HCG; Estrogen