Micropropagation of basil ‘Grecco a Palla’ mediated by 6-benzylaminopurine and 3-indole butyric acid

Gorski, Felipe; Gerotti, Geysiane Moreira; Magalhães, Hélida Mara

doi:10.1590/0103-8478cr20220375

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CROP PRODUCTION • Cienc. Rural 54 (3) • 2024 • https://doi.org/10.1590/0103-8478cr20220375 copy

Micropropagation of basil ‘Grecco a Palla’ mediated by 6-benzylaminopurine and 3-indole butyric acid

Micropropagação de manjericão ‘Grecco a palla’ mediado por 6-benzylaminopurine e 3-indole butyric acid

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT:

The present study evaluated the efficiency of a protocol for micropropagation of stem apexes and nodal segments of basil ‘Grecco a Palla’ in various concentrations of 6-benzylaminopurine (BAP) and 3-indole butyric acid (IBA). A completely randomized design was used with six treatments distributed in five replications. A medium without growth regulators favored the survival of Ocimumbasilicum stem apexes inoculated in vitro, and thereby promoted the sprouting of explants, whereas, for nodal segments, it was necessary to use regulators, and the concentration of 0.5 mg.L⁻¹ BAP 0.0 mg.L⁻¹ of IBA was more beneficial for the species.

Key words:
auxin; cytokinin; in vitro culture; Lamiaceae; Ocimum basilicum L

Authorship

Felipe Gorski

Laboratório de Cultura de Tecidos Vegetais, Universidade Paranaense (UNIPAR), 87502-210, Umuarama, PR, Brasil. Universidade Paranaense (UNIPAR)BrazilUmuarama, PR, BrazilLaboratório de Cultura de Tecidos Vegetais, Universidade Paranaense (UNIPAR), 87502-210, Umuarama, PR, Brasil.

Geysiane Moreira Gerotti

Hélida Mara Magalhães ^*E-mail: hmaramagal@gmail.com. *Corresponding author.

https://orcid.org/0000-0002-0402-7716

E-mail: hmaramagal@gmail.com. ^*Corresponding author.

Editors: Leandro Souza da Silva (0000-0002-1636-6643) Maria do Céu Monteiro Cruz (0000-0003-1293-1569)

DECLARATION OF CONFLICT OF INTEREST

The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.

AUTHORS’ CONTRIBUTIONS

All authors contributed equally to the design and writing of the manuscript. All authors critically reviewed the manuscript and approved the final version.

SCIMAGO INSTITUTIONS RANKINGS

Tables

Tables (2)

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Table 1
Average number of shoots (NS), average number of leaves (NL), root length (RL), shoot length (SL), root fresh matter (RFM), shoot fresh matter (SFM), relative chlorophyll index (RCI), relative phenol index (RPI), and relative anthocyanin index (RAI), obtained from stem apexes of Ocimumbasilicum ‘Grecco a Palla’ grown under different concentrations of 6-benzylaminopurine (BAP) and 3-indole butyric acid (IBA).

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Table 2
Average number of shoots (NS), average number of leaves (NL), root length (RL), shoot length (SL), root fresh matter (RFM), shoot fresh matter (SFM), relative chlorophyll index (RCI), relative phenol index (RPI) and relative anthocyanin index (RAI), obtained from nodal segments of Ocimumbasilicum ‘Grecco a Palla’ grown under different concentrations of 6-benzylaminopurine (BAP) and 3-indole butyric acid (IBA).

Table 1 Average number of shoots (NS), average number of leaves (NL), root length (RL), shoot length (SL), root fresh matter (RFM), shoot fresh matter (SFM), relative chlorophyll index (RCI), relative phenol index (RPI), and relative anthocyanin index (RAI), obtained from stem apexes of Ocimumbasilicum ‘Grecco a Palla’ grown under different concentrations of 6-benzylaminopurine (BAP) and 3-indole butyric acid (IBA).

Treatment	NS	NL	RL (mm)	SL (mm)	RFM (g)
A1	8.40±2.07^b*	135.00±10.95^a	88.38±12.33^ab	74.59±11.60^a	12.0173±1.60^a
A2	5.00±1.00^c	92.00±18.54^b	75.24±13.76^bc	63.70±7.92^a	10.1141±2.78^ab
A3	0.00^d	0.00^d	0.00^d	0.00^d	0.00^d
A4	7.00±0.00^b	78.00±11.31^b	97.02±6.76^a	53.15±19.73^ab	6.1624±3.91^bc
A5	3.66±0.57^c	151.67±11.93^a	47.05±3.95^d	51.33±8.56^b	4.6637±0.30^c
A6	18.33±1.52^a	119.67±3.78^a	62.86±12.67^cd	49.65±4.57^b	2.0605±0.03^d
Treatment	SFM (g)	RCI	RPI	RAI
A1	11.3971±0.42^a	31.46±1.90^a	0.45±0.04^a	0.27±0.03^a	- - -
A2	8.8207±1.14^b	0.94±2.96^d	0.30±0.07^b	0.26±0.01^a	- - -
A3	0.00^d	0.00^d	0.00^d	0.00^d	- - -
A4	5.5346±4.37^cd	9.78±2.57^c	0.20±0.02^c	0.23±0.02^ab	- - -
A5	6.5975±1.00^c	13.55±2.47^c	0.27±0.03^b	0.21±0.02^b	- - -
A6	4.1073±0.10^d	20.95±2.66^b	0.24±0.02^b	0.20±0.03^b	- - -

^*
Means ±standard deviation followed by the same letters in the column do not differ statistically by Tukey's test (P ≤ 0.05). A1: 0.0, 0.0; A2: 0.1, 0.0; A3: 0.5, 0.0; A4: 0.1, 0.05; A5: 0.05, 0.05; A6: 0.5, 0.1 mg.L^-1 of 6-benzylaminopurine (BAP) and 3-indole butyric acid (IBA),respectively.A3oxidation of all explants.

Table 2 Average number of shoots (NS), average number of leaves (NL), root length (RL), shoot length (SL), root fresh matter (RFM), shoot fresh matter (SFM), relative chlorophyll index (RCI), relative phenol index (RPI) and relative anthocyanin index (RAI), obtained from nodal segments of Ocimumbasilicum ‘Grecco a Palla’ grown under different concentrations of 6-benzylaminopurine (BAP) and 3-indole butyric acid (IBA).

Treatment	NS	NL	RL (mm)	SL (mm)	RFM (g)
NS1	6.50±0.70^c*	167.5±98.00^ab	84.41±44.44^b	85.73±8.70^b	7.9200±2.13^b
NS2	13.33±4.50^b	92.00±33.06^b	108.74±9.46^a	72.09±8.38^c	7.9054±2.49^b
NS3	17.00±1.41^a	127.5±68.68^ab	103.60±2.60^a	115.04±18.78^a	11.7913±0.34^a
NS4	10.50±2.12^b	94.00±7.07^b	90.04±35.41^ab	77.06±8.86^b	4.6167±0.37^c
NS5	12.66±1.15^b	132.67±22.23^a	71.05±7.92^b	62.82±6.28^c	6.9792±2.72^bc
NS6	18.66±5.50^ab	59.00±14.52^c	107.26±15.10ª	83.10±3.70^b	2.1651±0.16^d
Treatment	SFM (g)	RCI	RPI	RAI
NS1	10.9957±2.87^b	11.53±2.23^b	0.48±0.04^a	0.28±0.03^a	- - -
NS2	7.3360±2.49^d	13.02±2.49^ab	0.46±0.11^ab	0.26±0.03^a	- - -
NS3	11.0190±2.22^b	11.33±1.26^b	0.25±0.02^c	0.24±0.01^ab	- - -
NS4	14.5329±0.60^a	11.46±1.34^b	0.20±0.02^c	0.22±0.01^b	- - -
NS5	9.8110±1.40^bc	16.22±2.91^a	0.41±0.07^b	0.26±0.02^a	- - -
NS6	13.4195±5.85^ab	11.35±1.90^b	0.28±0.02^c	0.18±0.03^c	- - -

^*
Means ±standard deviation followed by the same letters in the column do not differ statistically by Tukey's test (P ≤ 0.05). NS1: 0.0, 0.0; NS2: 0.1, 0.0; NS3: 0.5, 0.0; NS4: 0.1, 0.05; NS5: 0.05, 0.05; NS6: 0.5, 0.1 mg.L-¹ of 6-benzylaminopurine (BAP) and 3-indole butyric acid (IBA),respectively.

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Micropropagation of basil ‘Grecco a Palla’ mediated by 6-benzylaminopurine and 3-indole butyric acid

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[1] E-mail: hmaramagal@gmail.com. ^*Corresponding author.