Micropropagação de manjericão ‘Grecco a palla’ mediado por 6-benzylaminopurine e 3-indole butyric acid

Gorski, Felipe; Gerotti, Geysiane Moreira; Magalhães, Hélida Mara

doi:10.1590/0103-8478cr20220375

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CROP PRODUCTION • Cienc. Rural 54 (3) • 2024 • https://doi.org/10.1590/0103-8478cr20220375 linkcopiar

Micropropagação de manjericão ‘Grecco a palla’ mediado por 6-benzylaminopurine e 3-indole butyric acid

Autoria SCIMAGO INSTITUTIONS RANKINGS

RESUMO:

O objetivo deste trabalho foi avaliar a eficiência de um protocolo de micropropagação de ápices caulinares e segmentos nodais da cultivar de manjericão (Ocimum basilicum L.) ‘Grecco a palla’ em diferentes concentrações de BAP (6-benzilaminopurina) e de AIB (ácido 3-indol butírico). Foi utilizado delineamento experimental inteiramente casualizado, com seis tratamentos distribuídos em cinco repetições. Para ápices caulinares, meio sem a adição de reguladores de crescimento favoreceu a sobrevivência de ápices caulinares de O. basilicum inoculados in vitro, promovendo a brotação dos explantes. Enquanto que para segmentos nodais houve necessidade do uso de reguladores, sendo que a concentração de 0,5 mg.L^-1 de BAP e 0,0 mg.L^-1 de AIB foi mais benéfica para a espécie.

Palavras-chave: auxinas; citocininas; cultivo in vitro; Lamiaceae; Ocimum basilicum L

Autoria

Felipe Gorski

Laboratório de Cultura de Tecidos Vegetais, Universidade Paranaense (UNIPAR), 87502-210, Umuarama, PR, Brasil. Universidade Paranaense (UNIPAR)BrazilUmuarama, PR, BrazilLaboratório de Cultura de Tecidos Vegetais, Universidade Paranaense (UNIPAR), 87502-210, Umuarama, PR, Brasil.

Geysiane Moreira Gerotti

Hélida Mara Magalhães ^*E-mail: hmaramagal@gmail.com. *Corresponding author.

https://orcid.org/0000-0002-0402-7716

E-mail: hmaramagal@gmail.com. ^*Corresponding author.

Editors: Leandro Souza da Silva (0000-0002-1636-6643) Maria do Céu Monteiro Cruz (0000-0003-1293-1569)

DECLARATION OF CONFLICT OF INTEREST

The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.

AUTHORS’ CONTRIBUTIONS

All authors contributed equally to the design and writing of the manuscript. All authors critically reviewed the manuscript and approved the final version.

SCIMAGO INSTITUTIONS RANKINGS

Tabelas

Tabelas (2)

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Table 1
Average number of shoots (NS), average number of leaves (NL), root length (RL), shoot length (SL), root fresh matter (RFM), shoot fresh matter (SFM), relative chlorophyll index (RCI), relative phenol index (RPI), and relative anthocyanin index (RAI), obtained from stem apexes of Ocimumbasilicum ‘Grecco a Palla’ grown under different concentrations of 6-benzylaminopurine (BAP) and 3-indole butyric acid (IBA).

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Table 2
Average number of shoots (NS), average number of leaves (NL), root length (RL), shoot length (SL), root fresh matter (RFM), shoot fresh matter (SFM), relative chlorophyll index (RCI), relative phenol index (RPI) and relative anthocyanin index (RAI), obtained from nodal segments of Ocimumbasilicum ‘Grecco a Palla’ grown under different concentrations of 6-benzylaminopurine (BAP) and 3-indole butyric acid (IBA).

table_chartTable 1 Average number of shoots (NS), average number of leaves (NL), root length (RL), shoot length (SL), root fresh matter (RFM), shoot fresh matter (SFM), relative chlorophyll index (RCI), relative phenol index (RPI), and relative anthocyanin index (RAI), obtained from stem apexes of Ocimumbasilicum ‘Grecco a Palla’ grown under different concentrations of 6-benzylaminopurine (BAP) and 3-indole butyric acid (IBA).

Treatment	NS	NL	RL (mm)	SL (mm)	RFM (g)
A1	8.40±2.07^b*	135.00±10.95^a	88.38±12.33^ab	74.59±11.60^a	12.0173±1.60^a
A2	5.00±1.00^c	92.00±18.54^b	75.24±13.76^bc	63.70±7.92^a	10.1141±2.78^ab
A3	0.00^d	0.00^d	0.00^d	0.00^d	0.00^d
A4	7.00±0.00^b	78.00±11.31^b	97.02±6.76^a	53.15±19.73^ab	6.1624±3.91^bc
A5	3.66±0.57^c	151.67±11.93^a	47.05±3.95^d	51.33±8.56^b	4.6637±0.30^c
A6	18.33±1.52^a	119.67±3.78^a	62.86±12.67^cd	49.65±4.57^b	2.0605±0.03^d
Treatment	SFM (g)	RCI	RPI	RAI
A1	11.3971±0.42^a	31.46±1.90^a	0.45±0.04^a	0.27±0.03^a	- - -
A2	8.8207±1.14^b	0.94±2.96^d	0.30±0.07^b	0.26±0.01^a	- - -
A3	0.00^d	0.00^d	0.00^d	0.00^d	- - -
A4	5.5346±4.37^cd	9.78±2.57^c	0.20±0.02^c	0.23±0.02^ab	- - -
A5	6.5975±1.00^c	13.55±2.47^c	0.27±0.03^b	0.21±0.02^b	- - -
A6	4.1073±0.10^d	20.95±2.66^b	0.24±0.02^b	0.20±0.03^b	- - -

^*
Means ±standard deviation followed by the same letters in the column do not differ statistically by Tukey's test (P ≤ 0.05). A1: 0.0, 0.0; A2: 0.1, 0.0; A3: 0.5, 0.0; A4: 0.1, 0.05; A5: 0.05, 0.05; A6: 0.5, 0.1 mg.L^-1 of 6-benzylaminopurine (BAP) and 3-indole butyric acid (IBA),respectively.A3oxidation of all explants.

table_chartTable 2 Average number of shoots (NS), average number of leaves (NL), root length (RL), shoot length (SL), root fresh matter (RFM), shoot fresh matter (SFM), relative chlorophyll index (RCI), relative phenol index (RPI) and relative anthocyanin index (RAI), obtained from nodal segments of Ocimumbasilicum ‘Grecco a Palla’ grown under different concentrations of 6-benzylaminopurine (BAP) and 3-indole butyric acid (IBA).

Treatment	NS	NL	RL (mm)	SL (mm)	RFM (g)
NS1	6.50±0.70^c*	167.5±98.00^ab	84.41±44.44^b	85.73±8.70^b	7.9200±2.13^b
NS2	13.33±4.50^b	92.00±33.06^b	108.74±9.46^a	72.09±8.38^c	7.9054±2.49^b
NS3	17.00±1.41^a	127.5±68.68^ab	103.60±2.60^a	115.04±18.78^a	11.7913±0.34^a
NS4	10.50±2.12^b	94.00±7.07^b	90.04±35.41^ab	77.06±8.86^b	4.6167±0.37^c
NS5	12.66±1.15^b	132.67±22.23^a	71.05±7.92^b	62.82±6.28^c	6.9792±2.72^bc
NS6	18.66±5.50^ab	59.00±14.52^c	107.26±15.10ª	83.10±3.70^b	2.1651±0.16^d
Treatment	SFM (g)	RCI	RPI	RAI
NS1	10.9957±2.87^b	11.53±2.23^b	0.48±0.04^a	0.28±0.03^a	- - -
NS2	7.3360±2.49^d	13.02±2.49^ab	0.46±0.11^ab	0.26±0.03^a	- - -
NS3	11.0190±2.22^b	11.33±1.26^b	0.25±0.02^c	0.24±0.01^ab	- - -
NS4	14.5329±0.60^a	11.46±1.34^b	0.20±0.02^c	0.22±0.01^b	- - -
NS5	9.8110±1.40^bc	16.22±2.91^a	0.41±0.07^b	0.26±0.02^a	- - -
NS6	13.4195±5.85^ab	11.35±1.90^b	0.28±0.02^c	0.18±0.03^c	- - -

^*
Means ±standard deviation followed by the same letters in the column do not differ statistically by Tukey's test (P ≤ 0.05). NS1: 0.0, 0.0; NS2: 0.1, 0.0; NS3: 0.5, 0.0; NS4: 0.1, 0.05; NS5: 0.05, 0.05; NS6: 0.5, 0.1 mg.L-¹ of 6-benzylaminopurine (BAP) and 3-indole butyric acid (IBA),respectively.

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