Osteoinductivity of demineralized homologous bone graft in rabbits

In order to evaluate osteoinduction of the homologous demineralized bone matrix (DBM), 1 cm radial fragments were demineralized in HCL 0.6 N solution for 24 hours, preserved in 70% ethanol solution and implanted in the abdominal musculature of 12 adult rabbits. The animals were sacrificed at 2, 4 and 6 post-operative weeks. After euthanasia, macroscopic, miroscopic and tetracycline fluorescence exams were performed. At four weeks, the implants already presented calcification signs. Microscopically, there was mature bone and medulary canal with hematopoietic cells, at six weeks. There was no evidence of rejection, or fluorescence at two and four weeks; at six weeks, there was difuse and concentric bands fluorescence. DBM in HCL 0.6 N solution and preserved in ethanol 70% solution maintains its osteoinductive property and remains sterile.

bone reparation; demineralized bone; bone graft