Limonium latifolium Kuntze is a cut flower commercialy propagated in vitro. To develop and improve the micropropagation protocol, a sequence of assays was developed to evaluate performance of node explants; effect of cytokinins concentration (kinetin-KlN and 6-benzyl-aminopurin-BA) on the regeneration rate; the presence of BA during the multiplication phase; concentration of naphthaleneacetic acid-NAA and indole3-butyric acid-IBA on the rooting phase and procederes for the acciimatiwtion in vitro. The micropropagation was done at commercial levei using inflorescence nades explants. By the regeneration phase the best results were obtained with BA at 0.7mg/l for 35 days in MS médium. The multiplication phase takes 35 days and shows satisfactory results with BA at 0.2mg/l , with the rate of 4 plantiets/explant. The rooting phase is takes 30 days in MS medium with IBA at 1mg/l , with good survival scores after transfer to in vivo. The acciimatiwtion hás made under plastic covering, inside greenhouse with room temperature and intermitent mist, using flats with 242 cells of 10cm³ each, filled with sterilized carbonized rice hulis. Every two weeks the plants were fertigated with commercial fertilizer (15:5:15 + micronutrients) at 0.5g/l . The in vitro process takes 100 - 120 days and one explant originates 15 - 30 plants.
Limonium latifolium; micropropagation; tissue culture
