The objective of this study was to establish a micropropagation protocol for Acca sellowiana (Berg) Burret. Nodal segments of in vitro cultivated plantlets of the genotypes 53B-7, 101, 529 and 152-12 x 458 were inoculated in test tubes containing 15ml of Woody Plant Medium-WPM solid medium supplemented with the cytokinins BAP, Kin, and 2-iP. For rooting purpose the microcuttings were submitted to different inductive periods of time with indolilbutiric acid-IBA (20 muM), then transferred to a basal solid medium free of growth regulators, or alternatively to ex vitro substrate in a phytotron. The three cytokinins did not improve the multiple shoot regeneration rates when compared with the control. The highest shoot height was observed in the WPM medium supplemented with Kin (5 muM). Nodal segments of the genotype 101, cultivated in WPM medium free of growth regulators showed higher regeneration rates than the other treatments. Six days pulses of IBA (20 muM) induced 68.9%, of microcutting rooting as well the highest values for root (5.6mm) and length number (1.3 roots). ex vitro rooting treatment of micropropagated microcuttings with IBA (100 muM) for 60min resulted in the highest values for plantlet height (45.3mm), secondary root number (11.3 roots), fresh (1069mg) and dry weight (282mg).of roots.
tissue culture; organogenesis; regeneration rate; in vitro and ex vitro rooting
