Serum samples from 304 dogs living in urban or rural areas of Monte Negro County, Rondônia State, Brazil, were evaluated for Brucella abortus reactivity using the Rose-Bengal Test (RBT), Standard Tube agglutination Test (SAT), and Mercaptoethanol Test (MET), and for Brucella canis reactivity using the Immunodiffusion test (AGID) and Immunodiffusion test with 2-Mercaptoethanol (ME-AGID). Serum was considered positive if reacted in the MET or ME-AGID. Fifty-six (18.4%) dogs reacted by the RBT, and 12 (4.0%) by the SAT. One (0.3%) dog was positive by the MET. Eleven (3.6%) dogs reacted by the AGID, but with no confirmation by the ME-AGID. It the small occurrence of dogs reacting by the MET and the absence of reactivity by ME-AGID is emphasized.
Brucella abortus; Brucella canis; dogs; Amazon; serology